摘要
目的探讨钌(Ⅱ)配合物[Ru(dmp)_2(NMIP)](ClO_4)_2对人骨肉瘤HOS细胞的凋亡作用初步作用机制。方法采用荧光定量PCR检测钌(II)配合物作用于人骨肉瘤HOS细胞后RNA变化,蛋白免疫电泳法检测钌(II)配合物作用于人骨肉瘤HOS细胞后细胞凋亡蛋白变化。结果钌配合物作用于HOS细胞24 h后,提取总基因逆转录后做荧光定量PCR。相比对照组,25、50、100μmol/L钌配合物组Bcl_2的mRNA表达明显降低(分别为0.86±0.02、0.52±0.01、0.4±0.01)(P<0.05),而Bax的mRNA表达明显升高(分别为1.18±0.18、1.7±0.10、1.69±0.10),Caspase3的mRNA表达明显升高(分别为1.22±0.11、1.61±0.12、1.62±0.06)。0、25、50、100μmol/L钌配合物能降低细胞体内Bcl2蛋白表达(分别为0.52±0.03、0.55±0.08、0.39±0.02、0.14±0.01),增加Bax蛋白表达(分别为0.52±0.01、0.60±0.02、0.64±0.03、0.62±0.02),增加Caspase3蛋白表达(分别为0.57±0.01、0.61±0.01、0.68±0.01、0.64±0.01)(P<0.05)。结论钌配合物通过线粒体引起的内源性凋亡通路引起骨肉瘤细胞凋亡。
Objective To investigate the preliminary apoptosis mechanism of Ruthenium(II) complex[Ru(dmp)_2(NMIP)](ClO_4)_2 on human osteosarcoma Hos cells. Methods RNA changes in human osteosarcoma HOS cells,treated with ruthenium(II)complex,were detected by QPCR,and apoptotic protein changes in human osteosarcoma Hos cells,treated with Ruthenium(II) complex,were detected by protein immunoelectrophoresis. Results After 24 hours of ruthenium complexes actingi in HOS cells,the total gene was retrieved and quantified by fluorescence quantitative PCR. Compared with the control group,the expression of Bcl2 in ruthenium complex group decreased significantly(P<0.05),while the expression of Bax and Caspase 3 increased significantly(P<0.05). Ruthenium complexes can decrease the expression of Bcl2 protein and increase the expression of Bax and Caspase 3 protein in cells. Conclusion Ruthenium complex could induce osteosarcoma cell apoptosis through endogenous apoptotic pathway induced by mitochondria.
引文
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