外翻肠囊法研究氧化苦参碱磷脂复合物的肠吸收特性
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摘要
目的通过对比研究氧化苦参碱及其磷脂复合物大鼠离体肠吸收情况,考察磷脂复合物对氧化苦参碱肠吸收的影响。方法采用大鼠离体外翻肠囊模型研究氧化苦参碱磷脂复合物的肠吸收特性。HPLC测定不同剂量的氧化苦参碱及氧化苦参碱磷脂复合物在大鼠十二指肠、空肠、回肠和结肠内的累积吸收量。结果氧化苦参碱磷脂复合物在不同肠段的吸收较氧化苦参碱均有提高,低剂量组氧化苦参碱磷脂复合物在空肠、结肠处的吸收较氧化苦参碱有显著提高;中剂量组氧化苦参碱磷脂复合物在十二指肠、空肠、结肠处的累积吸收量较氧化苦参碱有显著提高;高剂量组氧化苦参碱磷脂复合物在空肠的吸收较氧化苦参碱有显著提高。结论与氧化苦参碱相比,磷脂复合物对氧化苦参碱在大鼠肠道的吸收有显著的促进作用。
OBJECTIVE To compare the intestinal absorption of oxymatrine and its phospholipid complex in rats, and to investigate the effect of phospholipid complex on intestinal absorption of oxymatrine. METHODS The isolated everted intestine model was used to study the intestinal absorption characteristics of oxymatrine phospholipid complex. HPLC was used to determine the cumulative absorption of oxymatrine and oxymatrine phospholipid complex in rat duodenum, jejunum, ileum and colon with different dose. RESULTS The absorption of oxymatrine phospholipid complex in different intestinal segments was higher than that of oxymatrine. In the low dose group, the absorption of oxymatrine phospholipid complex in jejunum and colon was significantly higher than that of oxymatrine. In the middle dose group, the cumulative absorption of oxymatrine phospholipid complex in duodenum, jejunum and colon was significantly higher than that of oxymatrine. In the high dose group,the absorption of oxymatrine phospholipid complex in jejunum was significantly higher than that of oxymatrine.CONCLUSION Compared with oxymatrine, phospholipid complex can promote the absorption of oxymatrine in rats.
OBJECTIVE To compare the intestinal absorption of oxymatrine and its phospholipid complex in rats, and to investigate the effect of phospholipid complex on intestinal absorption of oxymatrine. METHODS The isolated everted intestine model was used to study the intestinal absorption characteristics of oxymatrine phospholipid complex. HPLC was used to determine the cumulative absorption of oxymatrine and oxymatrine phospholipid complex in rat duodenum, jejunum, ileum and colon with different dose. RESULTS The absorption of oxymatrine phospholipid complex in different intestinal segments was higher than that of oxymatrine. In the low dose group, the absorption of oxymatrine phospholipid complex in jejunum and colon was significantly higher than that of oxymatrine. In the middle dose group, the cumulative absorption of oxymatrine phospholipid complex in duodenum, jejunum and colon was significantly higher than that of oxymatrine. In the high dose group,the absorption of oxymatrine phospholipid complex in jejunum was significantly higher than that of oxymatrine.CONCLUSION Compared with oxymatrine, phospholipid complex can promote the absorption of oxymatrine in rats.
引文
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[2]LIU S,QIN J,LU W G,et al.Pharmacokinetics of oxymatrine in rats[J].Chin Tradit Patent Med(中成药),2011,33(6):962-965.
[3]GUO H,CHEN X M.Research progress of active constituents in Chinese herbal medicine[J].J Liaoning Univ TCM(辽宁中医药大学学报),2016,18(8):161-163.
[4]GUO H,CHEN X M,DUAN Z H.Study on the pharmacokinetics and relative bioavailability of enteric sustained-release capsule of oxymatrine phospholipid complex in mice[J].J Shandong Univ TCM(山东中医药大学学报),2017,41(5):482-485.
[5]DUAN Z H,CHEN X M,GUO H.Study on quality standard of enteric sustained-release capsules of oxymatrine phospholipid complex[J].J Shandong Univ TCM(山东中医药大学学报),2018,42(2):179-181.
[6]GAO Y,CHEN X M.Research on the physical characteristics of the contents of oxymatrine phospholipid complex enteric-coated capsule[J].Chin Med J Res Prac(现代中药研究与实践),2018,32(1):45-47,48.
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[8]DING F F,ZHANG X J,SUN Y,et al.Study on intestinal absorption of Jinqi Jiangtang tablets by rat everted gut sac[J].Chin J Hosp Pharm(中国医院药学杂志),2015,35(3):185-190.
[9]ZHANG Y F,LI Y J,YANG Q,et al.Rat Intestine absorption characteristics study on andrographolide with isolated everted intestine model[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,16(9):107-110.
[10]LI N,FENG L L,JIANG X H,et al.The absorption of baicalin phospholipid complex in rat stomach and intestine[J].Chin Pharm J(中国药学杂志),2016,51(12):994-998.
[11]LIU Z S,HAO H J,MA J N.Pharmacokinetics of lappaconitine-phospholipid complex and its solid dispersion in sd rats in vivo[J].Her Med(医药导报),2018,37(4):449-452.