金黄色葡萄球菌致急性与慢性输卵管炎性不孕症大鼠模型的建立
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摘要
目的利用金黄色葡萄球菌建立大鼠急性与慢性输卵管炎性不孕症模型。方法制备4×10~9/m L金黄色葡萄球菌悬液。将108只大鼠随机分为正常组、模型组、假手术组,每组36只。模型组经输卵管注入金葡菌悬液,假手术组经输卵管注入生理盐水;于术后第7、14、21、28天肉眼观察输卵管形态改变,行金黄色葡萄球菌培养及病理学检查。结果经输卵管注射金黄色葡萄球菌,可在术后第7天形成急性输卵管炎性模型,第21天形成慢性输卵管炎性模型,第28天形成输卵管炎性不孕症模型。结论采用浓度为4×10~9/m L金黄色葡萄球菌,注入大鼠输卵管,可成功建立急性输卵管炎与慢性输卵管炎性不孕症大鼠模型。
Objective To establish a model of acute and chronic oviduct inflammatory infertility in rats infected with Staphylococcus aureus. Methods A 4 × 10~9/m L S. aureus suspension was prepared. A total of 108 rats were randomly divided into a normal group,a model group,and a control group,with 36 rats in each. The model group was injected in the fallopian tube with the S. aureus suspension,and the control group was injected with saline in the fallopian tube. The morphology of the fallopian tube was observed on the 7 th,14 th,21 st,and 28 th days after surgery,and S.aureus culture and pathological examination were also performed. Results After the injection of S. aureus into the fallopian tube,acute oviduct inflammation emerged on postoperative days 7 and 21 to form chronic tubal inflammatory models,and on day 28 to form an oviduct inflammatory model of infertility. Conclusions The injection of 4 × 10~9/m L S.aureus into rat oviduct successfully established an acute salpingitis and chronic tubal phlogistic sex infertility rat model.
Objective To establish a model of acute and chronic oviduct inflammatory infertility in rats infected with Staphylococcus aureus. Methods A 4 × 10~9/m L S. aureus suspension was prepared. A total of 108 rats were randomly divided into a normal group,a model group,and a control group,with 36 rats in each. The model group was injected in the fallopian tube with the S. aureus suspension,and the control group was injected with saline in the fallopian tube. The morphology of the fallopian tube was observed on the 7 th,14 th,21 st,and 28 th days after surgery,and S.aureus culture and pathological examination were also performed. Results After the injection of S. aureus into the fallopian tube,acute oviduct inflammation emerged on postoperative days 7 and 21 to form chronic tubal inflammatory models,and on day 28 to form an oviduct inflammatory model of infertility. Conclusions The injection of 4 × 10~9/m L S.aureus into rat oviduct successfully established an acute salpingitis and chronic tubal phlogistic sex infertility rat model.
引文
[1]黄荷凤,王波,朱依敏.不孕症发生现状及趋势分析[J].中国实用妇科与产科杂志,2013,29(9):688-690.
[2]梁原.54例宫腔镜联合腹腔镜手术治疗不孕症的临床分析[J].中国现代医生,2010,48(11):117-118.
[3]欧阳林楠,卢苏.输卵管性不孕中医治疗进展[J].辽宁中医药大学学报,2015,17(3):207-209.
[4]陈晓.小鼠慢性输卵管炎症不孕模型制作[J].生殖与避孕,2015,35(10):670-673.
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[6]张翠莲.高龄女性二胎生育力评估及助孕策略[J].中华实用诊断与治疗杂志,2016,30(12):1145-1148.
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[9]彭端亮,李永文,赵鹃,等.淋病奈瑟菌、沙眼衣原体、解脲脲支原体感染与女性不孕症的关系[J].中华医院感染学杂志,2015,25(23):5494-5496.
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[13]Corr TE,Sullivan J,Frazer LC,et al.Steroids alone or as adjunctive therapy with doxycycline fail to improve oviduct damage in mice infected with Chlamydia muridarum[J].Clin Vaccine Immunol,2014,21(6):824-830.
[14]谢志波,梁志锋,朱小凤,等.甲醛复制小鼠输卵管炎性不孕症动物模型的研究[J].医学信息,2014,7(17):42.
[15]王芳军,吕江,王晓东,等.多模式输卵管性不孕症模型制作与初步应用研究[J].中华临床医师杂志,2015,9(10):1975-1978.
[16]李莉,郝秋芳.巨噬细胞分泌的效应分子在小鼠急性输卵管炎中的变化[J].中国急救医学,2000,20(4):200.
[17]Cox SM,Faro S,Dodson MG,et al.Role of Neisseria gonorrhoeae and Chlamydia trachomatis in intraabdominal abscess formation in the rat[J].J Reprod Med,1991,36(3):202-205.
[18]Pearlman MD,Mcneeley SG,Frank TS,et al.Antiendotoxin antibody is protective against tubal damage in an Escherichia coli rabbit salpingitis model[J].Am J Obstet Gynecol,1994,171(6):1588-1593.
[19]赵广兴,王春田,马宝璋,等.大鼠输卵管炎性不孕症模型的建立[J].中国比较医学杂志,2004,14(1):23-26.
[20]刘丽,李世大,赵丽燕.膈下逐瘀冲剂对输卵管炎性不孕大鼠NF-κB、IFN-γ蛋白表达的影响[J].世界中西医结合杂志,2014,9(3):251-253.
[21]陈晓,王凤伟.TLR2和TNF-α与输卵管炎的相关性研究[J].生殖医学杂志,2016,25(1):56-60.
[2]梁原.54例宫腔镜联合腹腔镜手术治疗不孕症的临床分析[J].中国现代医生,2010,48(11):117-118.
[3]欧阳林楠,卢苏.输卵管性不孕中医治疗进展[J].辽宁中医药大学学报,2015,17(3):207-209.
[4]陈晓.小鼠慢性输卵管炎症不孕模型制作[J].生殖与避孕,2015,35(10):670-673.
[5]徐晓群,王欢,吕火烊.金黄色葡萄球菌溶血素的研究进展[J].中国微生态学杂志,2017,29(6):720-724.
[6]张翠莲.高龄女性二胎生育力评估及助孕策略[J].中华实用诊断与治疗杂志,2016,30(12):1145-1148.
[7]符丽,刘琴,潘小敏,等.输卵管炎性不孕中西医认识[J].亚太传统医药,2016,12(8):52-53.
[8]姚晔,万琼,李志红,等.345例支原体阳性患者病原体分布及药敏分析[J].实验与检验医学,2010,28(1):84-85.
[9]彭端亮,李永文,赵鹃,等.淋病奈瑟菌、沙眼衣原体、解脲脲支原体感染与女性不孕症的关系[J].中华医院感染学杂志,2015,25(23):5494-5496.
[10]沈秀珍,李兰芳.女性生殖道支原体、衣原体及淋球菌感染与不孕症的相关性分析[J].中国妇幼保健,2016,31(6):1236-1237.
[11]Chambers HF,Deleo FR.Waves of resistance:Staphylococcus aureus in the antibiotic era[J].Nat Rev Microbiol,2009,7(9):629-641.
[12]冯子聪,张昭,黎哲,等.大肠埃希菌致兔慢性输卵管炎症模型的建立[J].中国比较医学杂志,2016,26(3):19-23.
[13]Corr TE,Sullivan J,Frazer LC,et al.Steroids alone or as adjunctive therapy with doxycycline fail to improve oviduct damage in mice infected with Chlamydia muridarum[J].Clin Vaccine Immunol,2014,21(6):824-830.
[14]谢志波,梁志锋,朱小凤,等.甲醛复制小鼠输卵管炎性不孕症动物模型的研究[J].医学信息,2014,7(17):42.
[15]王芳军,吕江,王晓东,等.多模式输卵管性不孕症模型制作与初步应用研究[J].中华临床医师杂志,2015,9(10):1975-1978.
[16]李莉,郝秋芳.巨噬细胞分泌的效应分子在小鼠急性输卵管炎中的变化[J].中国急救医学,2000,20(4):200.
[17]Cox SM,Faro S,Dodson MG,et al.Role of Neisseria gonorrhoeae and Chlamydia trachomatis in intraabdominal abscess formation in the rat[J].J Reprod Med,1991,36(3):202-205.
[18]Pearlman MD,Mcneeley SG,Frank TS,et al.Antiendotoxin antibody is protective against tubal damage in an Escherichia coli rabbit salpingitis model[J].Am J Obstet Gynecol,1994,171(6):1588-1593.
[19]赵广兴,王春田,马宝璋,等.大鼠输卵管炎性不孕症模型的建立[J].中国比较医学杂志,2004,14(1):23-26.
[20]刘丽,李世大,赵丽燕.膈下逐瘀冲剂对输卵管炎性不孕大鼠NF-κB、IFN-γ蛋白表达的影响[J].世界中西医结合杂志,2014,9(3):251-253.
[21]陈晓,王凤伟.TLR2和TNF-α与输卵管炎的相关性研究[J].生殖医学杂志,2016,25(1):56-60.