膈下逐瘀汤加减方对气滞血瘀型输卵管炎大鼠模型TNF-α蛋白表达的影响
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摘要
目的研究应用多种刺激联合输卵管注菌的方法、制造出符合中医证候特点的气滞血瘀型输卵管炎大鼠模型,观察通过膈下逐瘀加减方治疗对该模型大鼠TNF-α蛋白表达的影响。方法将48只Wistar雌性大鼠随机分为4组,每组12只,分别为正常组、模型组(气滞血瘀型输卵管炎组)、成药组(康妇炎胶囊组)、中药组(膈下逐瘀汤加减方组)。正常对照组予以普通喂养,模型组给予输卵管注菌造模,并采用多种刺激干预处理以造成气滞血瘀型输卵管炎不孕大鼠模型,并观察大鼠输卵管及外部体征变化情况。成药组与中药组:即经过康妇炎胶囊以及膈下逐瘀汤加减方治疗后,观察气滞血瘀型输卵管炎大鼠的TNF-α蛋白表达情况的不同。结果模型组出现气滞血瘀型输卵管炎的症状,输卵管与周围组织黏连,显微镜下观察输卵管形态改变、增粗、积水,炎症指标TNF-α差异比较,有统计学意义(P<0.05)。结论多种刺激联合输卵管注菌建立的气滞血瘀型输卵管炎模型,符合输卵管炎病理特点和中医气滞血瘀证候的主要辨证要点,是较为理想的气滞血瘀型输卵管炎模型。膈下逐瘀汤加减方能抑制输卵管上皮TNF-α蛋白的表达,强度优于成药组。
Objective To explore the method of combined tubal injection stimulation on the application of a variety of bacteria, producing model of arthritis in rats with qi stagnation and blood stasis syndrome characteristics of fallopian tube, and through the observation of Gexia Zhuyu decoction on the treatment effect of the expression of TNF-α protein in model rats. Methods 48 Wistar female rats were randomly divided into 4 groups, 12 in each group. They were normal group, model group(qi stagnation and blood stasis type salpingitis group), medicine group(Kangfuyan capsule group), Chinese medicine group(Gexia Zhuyu decoction). The normal control group was given general feeding, the model group was given the salpingo injection, and the multiple stimulation intervention was applied to induce the tubal inflammation infertility model of qi stagnation and blood stasis type, and the changes of the oviduct and external signs were observed. After the treatment of Kangfuyan capsule and Gexia Zhuyu decoction, the expression of TNF-α protein in rats with salpingitis induced by qi stagnation and blood stasis was observed. Results In the model group, there were symptoms of salpingitis with qi stagnation and blood stasis, and the adhesion between the oviduct and the surrounding tissues. The morphological changes, thickening and hydropsis of the fallopian tubes and inflammatory index TNF-α were observed by microscope, and the difference was statistically significant(P < 0.05). Conclusion The model of tubal inflammation with qi stagnation and blood stasis established by multiple stimulation combined with oviduct injection is consistent with the pathological characteristics of salpingitis and the main dialectical points of TCM qi stagnation and blood stasis syndrome. It is an ideal tubal inflammation model with qi stagnation and blood stasis. The expression intensity of TNF-α protein in tubal epithelium is better than that of the group of Kangfuyan capsule.
Objective To explore the method of combined tubal injection stimulation on the application of a variety of bacteria, producing model of arthritis in rats with qi stagnation and blood stasis syndrome characteristics of fallopian tube, and through the observation of Gexia Zhuyu decoction on the treatment effect of the expression of TNF-α protein in model rats. Methods 48 Wistar female rats were randomly divided into 4 groups, 12 in each group. They were normal group, model group(qi stagnation and blood stasis type salpingitis group), medicine group(Kangfuyan capsule group), Chinese medicine group(Gexia Zhuyu decoction). The normal control group was given general feeding, the model group was given the salpingo injection, and the multiple stimulation intervention was applied to induce the tubal inflammation infertility model of qi stagnation and blood stasis type, and the changes of the oviduct and external signs were observed. After the treatment of Kangfuyan capsule and Gexia Zhuyu decoction, the expression of TNF-α protein in rats with salpingitis induced by qi stagnation and blood stasis was observed. Results In the model group, there were symptoms of salpingitis with qi stagnation and blood stasis, and the adhesion between the oviduct and the surrounding tissues. The morphological changes, thickening and hydropsis of the fallopian tubes and inflammatory index TNF-α were observed by microscope, and the difference was statistically significant(P < 0.05). Conclusion The model of tubal inflammation with qi stagnation and blood stasis established by multiple stimulation combined with oviduct injection is consistent with the pathological characteristics of salpingitis and the main dialectical points of TCM qi stagnation and blood stasis syndrome. It is an ideal tubal inflammation model with qi stagnation and blood stasis. The expression intensity of TNF-α protein in tubal epithelium is better than that of the group of Kangfuyan capsule.
引文
[1]谢幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2013:369.
[2]李炜,段金廒,刘培,等.采用雌性SD大鼠探索建立适宜于气滞血瘀证妇科疾病特点的模型评价体系[J].中国中药杂志,2013,(8):1211-1218.
[3]毕京峰,王鈜,陈大为,等.药物临床试验中“医”“患”权益保障所面临的问题与挑战[J].中国临床药理学与治疗学,2014,19(3):331-333.
[4]崔阳阳,孙伟伟,赵瑞华.子宫内膜异位症动物模型研究进展[J].中国实验动物学报,2013,21(5):86-89,94.
[5]徐桂中,张欣,侯一平,等.金黄色葡萄球菌致小鼠慢性输卵管炎性不孕模型制作[J].中国比较医学杂志,2011,21(3):6-9.
[6]任建勋,林成仁,王敏,等.多因素整合建立气滞血瘀证动物模型研究[J].中药药理与临床,2007,23(5):210-211.
[7]陈天娥,王秀琴,孙海梅,等.大鼠脾气虚胃溃疡证病结合模型肠系膜淋巴结表达IL-2的初步研究[J].首都医科大学学报,2004,25(3):302-306.
[8]时敬文,陆启滨.中医内治法治疗输卵管性不孕症研究[J].长春中医药大学学报,2012,28(1):101-102.
[9]蔡云菲,夏亲华.输卵管性不孕症的临床治疗[J].长春中医药大学学报,2015,31(1):93-95,104.
[10]陈国珍.活血调经助孕汤治疗输卵管阻塞性不孕60例[J].吉林中医药,2006,26(1):31.
[11]叶红.膈下逐瘀汤加减治疗气滞血瘀型原发性痛经的临床观察[D].长春:长春中医药大学,2009.
[12]王忠.膈下逐瘀汤加减治愈输卵管阻塞不通16例[J].基层医学论坛,2008,12(13):449-450.
[13]赵丽燕.膈下逐瘀汤冲剂治疗不孕症之输卵管炎的临床疗效观察[D].哈尔滨:黑龙江中医药大学,2014.
[14]王慧玲.肿瘤坏死因子-α与基质金属蛋白酶-9在输卵黏膜上皮的表达及与输卵管妊娠的关系[D].苏州:苏州大学,2010.
[15]武孟香,郭艳萍,许燕丽.TLR4和TNF-α在输卵管炎中的表达和意义[J].齐齐哈尔医学院学报,2011(32):3616-3617.
[2]李炜,段金廒,刘培,等.采用雌性SD大鼠探索建立适宜于气滞血瘀证妇科疾病特点的模型评价体系[J].中国中药杂志,2013,(8):1211-1218.
[3]毕京峰,王鈜,陈大为,等.药物临床试验中“医”“患”权益保障所面临的问题与挑战[J].中国临床药理学与治疗学,2014,19(3):331-333.
[4]崔阳阳,孙伟伟,赵瑞华.子宫内膜异位症动物模型研究进展[J].中国实验动物学报,2013,21(5):86-89,94.
[5]徐桂中,张欣,侯一平,等.金黄色葡萄球菌致小鼠慢性输卵管炎性不孕模型制作[J].中国比较医学杂志,2011,21(3):6-9.
[6]任建勋,林成仁,王敏,等.多因素整合建立气滞血瘀证动物模型研究[J].中药药理与临床,2007,23(5):210-211.
[7]陈天娥,王秀琴,孙海梅,等.大鼠脾气虚胃溃疡证病结合模型肠系膜淋巴结表达IL-2的初步研究[J].首都医科大学学报,2004,25(3):302-306.
[8]时敬文,陆启滨.中医内治法治疗输卵管性不孕症研究[J].长春中医药大学学报,2012,28(1):101-102.
[9]蔡云菲,夏亲华.输卵管性不孕症的临床治疗[J].长春中医药大学学报,2015,31(1):93-95,104.
[10]陈国珍.活血调经助孕汤治疗输卵管阻塞性不孕60例[J].吉林中医药,2006,26(1):31.
[11]叶红.膈下逐瘀汤加减治疗气滞血瘀型原发性痛经的临床观察[D].长春:长春中医药大学,2009.
[12]王忠.膈下逐瘀汤加减治愈输卵管阻塞不通16例[J].基层医学论坛,2008,12(13):449-450.
[13]赵丽燕.膈下逐瘀汤冲剂治疗不孕症之输卵管炎的临床疗效观察[D].哈尔滨:黑龙江中医药大学,2014.
[14]王慧玲.肿瘤坏死因子-α与基质金属蛋白酶-9在输卵黏膜上皮的表达及与输卵管妊娠的关系[D].苏州:苏州大学,2010.
[15]武孟香,郭艳萍,许燕丽.TLR4和TNF-α在输卵管炎中的表达和意义[J].齐齐哈尔医学院学报,2011(32):3616-3617.