鹿茸-山药药对补肾壮阳作用研究
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摘要
目的:基于鹿茸-山药药对补肾壮阳活性,探讨鹿茸-山药药对对肾阳虚大鼠的治疗作用。方法:采用雄性Wistar大鼠90只,随机分为空白对照组、模型组[注射氢化可的松2.5 mg·(100 g)~(-1)]、中药阳性药组(男宝胶囊0.6 g·kg~(-1))、西药阳性药组(丙酸睾酮,10 mg·kg~(-1))、鹿山高剂量组(1.8 g生药·kg~(-1))、鹿山中剂量组(0.9 g生药·kg~(-1))、鹿山低剂量组(0.45 g生药·kg~(-1))、鹿茸组(0.4 g生药·kg~(-1))及山药组(1.4 g生药·kg~(-1)),每组10只,于末次给药后测定各组大鼠体质量、血清中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)、促性腺激素释放激素(GnRH)、肾上腺皮质酮(CORT)水平、前列腺系数、提肛肌+储精囊系数。结果:与空白对照组相比,模型组大鼠体质量与血清中cAMP、cAMP/cGMP、T、FSH、LH、GnRH、CORT水平均显著降低(P<0.05或P<0.01),前列腺系数、提肛肌+储精囊系数均显著下降(P<0.05);与模型组比较,中药阳性药组血清cAMP、cAMP/cGMP、T、FSH水平与前列腺系数均显著升高(P<0.05),西药阳性药组FSH水平、LH水平、提肛肌+储精囊系数显著增加(P<0.05),鹿茸组T、CORT、GnRH水平升高(P<0.05),山药组FSH水平显著提高(P<0.05),鹿山高剂量组cAMP、cAMP/cGMP、FSH、LH水平与前列腺系数、提肛肌+储精囊系数提高(P<0.05),鹿山低剂量组大鼠体质量、LH水平显著升高(P<0.05)。结论:鹿茸-山药药对具有补肾壮阳作用,其机制可能是通过二者的协同作用增加cAMP调节垂体-性腺轴功能提高FSH、LH水平与前列腺系数及提肛肌+储精囊系数实现的。
Objective:To study the effects of Antler-Chinese yam on kidney yang deficiency rat.Methods:Ninety male rats were randomly divided into blank control group,model control group[injection of hydrocortisone 2.5 mg·(100 g)~(-1)],traditional Chinese medicine control group(0.6 g·kg~(-1)),western medicine control group(testosterone propionate,10 mg·kg~(-1)),high dose group of Antler-Chinese yam(1.8 g raw drug·kg~(-1)),and middle dose group(0.9 g raw drug·kg~(-1)),low dose group(0.45 g raw drug·kg~(-1)),antler group(0.4 g raw drug·kg~(-1))and yam group(1.4 g raw drug·kg~(-1)).The influence of each samples on the body weight,serum hormone level and sex organs of hydrocortisone induced Yang deficiency rats were measured.Results:Compared with the blank group,the body weight,serum levels of cAMP and cAMP/cGMP,T,FSH,LH,GnRH and CORT in the model group decreased significantly(P<0.05 or P<0.01),and the prostate coefficient,levator ani muscle and seminal vesicle coefficient decreased significantly(P<0.05).Compared with the model group,the levels of serum cAMP,cAMP/cGMP,T,FSH and prostate coefficient were significantly increased in the Chinese medicine positive group(P<0.05),FSH,LH,coefficient of levator ani muscle and seminal vesicle were significantly increased in the western medicine positive group(P<0.05),T,CORT,GnRH levels in the pilose antler group were significantly increased(P<0.05),and FSH levels in the yam group were significantly increased(P<0.05).The levels of cAMP,cAMP/cGMP,FSH,LH,coefficient of prostate coefficient,levator ani muscle and seminal vesicle in Antler-Chinese yam high dose group were increased(P<0.05),while the body weight and LH levels in Lushan low dose group were significantly increased(P<0.05).Conclusion:The Antler-Chinese yam has the effect of invigorating kidney yang,which may be the synergistic effect of the two drugs to regulate the pituitary-gonadal axis function by increasing the content of cAMP,and improve the level of FSH,LH and the coefficient of gonadal gland.
Objective:To study the effects of Antler-Chinese yam on kidney yang deficiency rat.Methods:Ninety male rats were randomly divided into blank control group,model control group[injection of hydrocortisone 2.5 mg·(100 g)~(-1)],traditional Chinese medicine control group(0.6 g·kg~(-1)),western medicine control group(testosterone propionate,10 mg·kg~(-1)),high dose group of Antler-Chinese yam(1.8 g raw drug·kg~(-1)),and middle dose group(0.9 g raw drug·kg~(-1)),low dose group(0.45 g raw drug·kg~(-1)),antler group(0.4 g raw drug·kg~(-1))and yam group(1.4 g raw drug·kg~(-1)).The influence of each samples on the body weight,serum hormone level and sex organs of hydrocortisone induced Yang deficiency rats were measured.Results:Compared with the blank group,the body weight,serum levels of cAMP and cAMP/cGMP,T,FSH,LH,GnRH and CORT in the model group decreased significantly(P<0.05 or P<0.01),and the prostate coefficient,levator ani muscle and seminal vesicle coefficient decreased significantly(P<0.05).Compared with the model group,the levels of serum cAMP,cAMP/cGMP,T,FSH and prostate coefficient were significantly increased in the Chinese medicine positive group(P<0.05),FSH,LH,coefficient of levator ani muscle and seminal vesicle were significantly increased in the western medicine positive group(P<0.05),T,CORT,GnRH levels in the pilose antler group were significantly increased(P<0.05),and FSH levels in the yam group were significantly increased(P<0.05).The levels of cAMP,cAMP/cGMP,FSH,LH,coefficient of prostate coefficient,levator ani muscle and seminal vesicle in Antler-Chinese yam high dose group were increased(P<0.05),while the body weight and LH levels in Lushan low dose group were significantly increased(P<0.05).Conclusion:The Antler-Chinese yam has the effect of invigorating kidney yang,which may be the synergistic effect of the two drugs to regulate the pituitary-gonadal axis function by increasing the content of cAMP,and improve the level of FSH,LH and the coefficient of gonadal gland.
引文
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[23] 史辑,景海漪,黄玉秋,等.巴戟天及其不同炮制品对脾肾阳虚模型大鼠的改善作用比较[J].中国药房,2016,27(13):1756-1758.
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[28] 黎同明,高洁,贝毓.鹿茸及鹿鞭对肾阳虚大鼠不育症的实验研究[J].广州中医药大学学报,2011,28(4):406-408.
[29] 张红梅.人参与鹿茸配伍对肾阳虚大鼠生殖机能影响的实验研究[D].沈阳:辽宁中医学院,辽宁中医药大学,2002.
[30] 闵建新,王建红,伍庆华,等.肾阳虚大鼠垂体-肾上腺皮质轴与垂体-性腺轴相关性研究[J].时珍国医国药,2008,19(11):2576-2577.
[31] 王秀凤,张磊,伍庆华,等.基于支持向量回归机制的肾阳虚大鼠垂体-肾上腺轴与垂体-性腺轴功能动态分析[J].中国中医基础医学杂志,2014,20(11):1487-1489.
[2] 牛晓晖,宗颖,孙佳明,等.鹿茸温肾壮阳作用机理研究概述[J].吉林中医药,2012,32(12):1254-1256.
[3] 王楠,高晓霞,代子彦,等.鹿茸药效物质基础、药理作用、临床应用及质量控制的研究进展[J].中草药,2017,48(22):4784-4790.
[4] ZANG Z J,TANG H F,YING T,et al.Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice:[J].Asian J Androl,2016,18(4):613.
[5] HU W,QI L,TIAN Y H,et al.Studies on the purification of polypeptide from sika antler plate and activities of antitumor[J].Bmc Complem Altern M,2015,15(1):1-8.
[6] NA L,MIN Z,DRUMMEN G P C,et al.Sika Deer Antler Collagen Type I-Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway[J].Evid-Based Complement Alternat Med,2016(2):1-13.
[7] SHI H,TAO Y,LI Z,et al.Bone regeneration strategy inspired by the study of calcification behavior in deer antler[J].Mater Sci Eng C Mater Biol Appl,2015,57:67-76.
[8] PARK Y,CHOI H S,LEE H S,et al.Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells[J].Nutr Res Pract,2015,9(5):451-458.
[9] 张岩荣,董诚明,薛南南,等.不同等级山药种栽与其内源激素(IAA、GA_3)含量及蔗糖酶活性之间相关性研究[J].中国现代中药,2017,19(3):393-397.
[10] YANG W,YING W,LI X,et al.Purification and structural characterization of Chinese yam polysaccharide and its activities[J].Carbohyd Polym,2015,117(5):1021.
[11] JHENG Y J,TSAI W Y,CHEN K H,et al.Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities[J].Protein Expres Purif,2012,85(1):77-85.
[12] CHEN Y F,ZHU Q,WU S.Preparation of oligosaccharides from Chinese yam and their antioxidant activity[J].Food Chem,2015,173:1107-1110.
[13] LIU Y,LI H,FAN Y,et al.Antioxidant and Antitumor Activities of the Extracts from Chinese Yam(Dioscorea opposite Thunb.)Flesh and Peel and the Effective Compounds[J].J Food Sci,2016,81(6):H1553-H1564.
[14] ZENG M,ZHANG L,LI M,et al.Estrogenic Effects of the Extracts from the Chinese Yam(Dioscorea opposite Thunb.)and Its Effective Compounds in vitro and in vivo[J].Molecules,2018,23(2):11.
[15] 杨鸿珠,龚雨萍.中药药对的研究进展与思考[J].中国中西医结合杂志,2010,30(2):218-220.
[16] 唐于平,束晓云,李伟霞,等.药对研究(Ⅰ)-药对的形成与发展[J].中国中药杂志,2013,38(24):4185-4190.
[17] 牛晓晖,张海莲,孙佳明,等.鹿茸-山药药对的补虚作用实验研究[J].中国实验方剂学杂志,2013,19(4):232-235.
[18] 赵罗娜,张永萍,刘明,等.新生精胶囊对氢化可的松致小鼠肾阳虚的影响[J].微量元素与健康研究,2018,35(4):1-3.
[19] 陆翔恩,罗春丽,赵致,等.苗药双肾草提取物对肾阳虚小鼠的影响[J].中国现代中药,2014,16(5):380-383.
[20] 戴冰,张嘉妮,杨梦琳,等.氢化可的松致肾虚证小鼠模型的建立及相关指标的评价[J].中国实验动物学报,2017,25(1):70-73.
[21] 范润勇,王琳,黄勤挽,等.中药炮天雄对腺嘌呤致肾阳虚模型大鼠的治疗作用研究[J].亚太传统医药,2018,14(6):16-20.
[22] 欧莉.基于不同肾虚证候的何首乌致肝损伤机制研究[D].成都:成都中医药大学,2016.
[23] 史辑,景海漪,黄玉秋,等.巴戟天及其不同炮制品对脾肾阳虚模型大鼠的改善作用比较[J].中国药房,2016,27(13):1756-1758.
[24] 张帆,曾灵.肾阳虚型围绝经模型大鼠构建及其下丘脑-垂体-性腺轴激素水平研究[J].中医临床研究,2016,8(7):7-9.
[25] 苟小军,韩宝侠,王朝廷,等.肾阳虚证造模方法考察[J].吉林中医药,2009,29(9):814-815.
[26] 刘源才,杨跃军,冯声宝,等.中国劲酒对肾阳虚模型大鼠下丘脑-垂体-肾上腺轴及免疫功能的影响[J].中国免疫学杂志,2011,27(12):1080-1084.
[27] 黎慧娟,高常青,胡平安.运动与下丘脑-垂体-睾丸轴[J].北方药学,2012,9(4):28-30.
[28] 黎同明,高洁,贝毓.鹿茸及鹿鞭对肾阳虚大鼠不育症的实验研究[J].广州中医药大学学报,2011,28(4):406-408.
[29] 张红梅.人参与鹿茸配伍对肾阳虚大鼠生殖机能影响的实验研究[D].沈阳:辽宁中医学院,辽宁中医药大学,2002.
[30] 闵建新,王建红,伍庆华,等.肾阳虚大鼠垂体-肾上腺皮质轴与垂体-性腺轴相关性研究[J].时珍国医国药,2008,19(11):2576-2577.
[31] 王秀凤,张磊,伍庆华,等.基于支持向量回归机制的肾阳虚大鼠垂体-肾上腺轴与垂体-性腺轴功能动态分析[J].中国中医基础医学杂志,2014,20(11):1487-1489.