邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物对S.Pombe活性的影响研究
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摘要
本文采用液相合成法,以邻香草醛、甘氨酸、二水氯化铜为原料合成邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物,并利用红外光谱、元素分析等分析方法对合成的目标产物进行表征.再应用TAM Air微量热仪分别测得在305.15 K条件下不同浓度邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物作用的S.pombe生长产热曲线,并依据产热曲线计算出S.pombe生长的传代时间t_G、生长代谢速率常数k、最大发热功率所需时间t_(max)和抑制率I等热动力学参数.实验结果表明,随着邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物浓度的增加,S.pombe的生长代谢速率常数k依次减少,而最大功率所需时间t_(max)、传代时间t_G和抑制率I则依次增加,且邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物的半抑制浓度C_(I,50)分别为2.01×10~(-3) mol·L~(-1)、7.34×10~(-3) mol·L~(-1),即邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)配合物对S.pombe的生长均具有较好的抑制作用.
In this paper,the synthesis of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex were synthesized by liquid phase synthesis with the raw materials of o-vanillin, glycine and copper chloride dihydrate. Elemental analysis and IR spectroscopy were employed to characterize the composition and structure of the synthesized target products.The growth thermogenic curve of S.Pombe with different concentrations of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex was measured by TAM Air microcalorimeter at 305.15 K.According to the heat production curve,the thermodynamic parameters such as the generation time(t_G),growth rate constant(k),maximum heating power time(t_(max)) and inhibition ratio(I) of S.Pombe growth were calculated.The experimental results indicate that the value of k of S.Pombe decrease,while the values of tmax, t_G and I of S.Pombe increased with the increase of the concentrations of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex,and the half inhibitory concentrations(C_(I,50)) value of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex were found to be 2.01×10~(-3) mol·L~(-1) and 7.34×10~(-3) mol·L~(-1),respectively and o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex had good inhibition action on the growth of S.Pombe.
In this paper,the synthesis of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex were synthesized by liquid phase synthesis with the raw materials of o-vanillin, glycine and copper chloride dihydrate. Elemental analysis and IR spectroscopy were employed to characterize the composition and structure of the synthesized target products.The growth thermogenic curve of S.Pombe with different concentrations of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex was measured by TAM Air microcalorimeter at 305.15 K.According to the heat production curve,the thermodynamic parameters such as the generation time(t_G),growth rate constant(k),maximum heating power time(t_(max)) and inhibition ratio(I) of S.Pombe growth were calculated.The experimental results indicate that the value of k of S.Pombe decrease,while the values of tmax, t_G and I of S.Pombe increased with the increase of the concentrations of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex,and the half inhibitory concentrations(C_(I,50)) value of o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex were found to be 2.01×10~(-3) mol·L~(-1) and 7.34×10~(-3) mol·L~(-1),respectively and o-vanillin-glycine Schiff base and its Cu(Ⅱ) complex had good inhibition action on the growth of S.Pombe.
引文
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[10] 杨德俊,李旭,胡吉林,等.烟酸锰配合物[Mn(C6H4NO2)2]·2H2O(s)的合成、生成焓及其对粟酒裂殖酵母细胞生长代谢的微量热法研究[J].化学学报,2010,68(23):2373-2380.
[11] 蒋建宏,李旭,肖圣雄,等.2-{[4-氨基-5-(3,4,5-三甲氧基-苄基)-嘧啶-2-亚胺基]-甲基}-6-甲氧基-苯酚与酵母细胞和牛血清白蛋白的相互作用[J].高等学校化学学报,2014,35(4):831-838.
[2] 刘玉婷,张洁心,尹大伟.氨基酸Schiff碱及其金属配合物的性能研究进展[J].氨基酸和生物资源,2008,30(3):51-54.
[3] 冯小强,李小芳,朱元成.邻香草醛缩壳寡糖席夫碱及其Cu(Ⅱ)配合物与DNA的相互作用[J].吉林大学学报,2018,56(3):732-737.
[4] 李旭,蒋建宏,韩布兴,等.邻香草醛缩组氨酸Schiff碱及其镧配合物的合成与生物活性[J].高等学校化学学报,2015,36(5):856-863.
[5] 廖丰华,伍徐孟,张丽,等.稀土钐邻香草醛缩甘氨酸席夫碱配合物的合成及表征[J].邵阳学院学报,2015,12(2):39-44.
[6] 蒋建宏,李旭,谷惠文,等.邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究[J].井冈山大学学报,2015,36(6):29-35.
[7]PRABHAKARAN R,GEETHA A,THILAGAVATHI M,et al.Synthesis,characterization,EXAFS investigation and antibacterial activities of new ruthenium(III) complexes containing tetradentate Schiff base[J].Inorg Biochem,2004,98:2131-2140.
[8] ASSEGID G,LAMPRECHT I.Microcalorimetric investigations on the influence of propolis on the bacterium micrococcus luteus[J].Thermochim Acta,1997,290(2):155-166.
[9] 李幸群.邻香草醛甘氨酸席夫碱及其Cu(Ⅱ)络合物的合成与表征[J].华中师范大学学报,1990(S2):141-144.
[10] 杨德俊,李旭,胡吉林,等.烟酸锰配合物[Mn(C6H4NO2)2]·2H2O(s)的合成、生成焓及其对粟酒裂殖酵母细胞生长代谢的微量热法研究[J].化学学报,2010,68(23):2373-2380.
[11] 蒋建宏,李旭,肖圣雄,等.2-{[4-氨基-5-(3,4,5-三甲氧基-苄基)-嘧啶-2-亚胺基]-甲基}-6-甲氧基-苯酚与酵母细胞和牛血清白蛋白的相互作用[J].高等学校化学学报,2014,35(4):831-838.