辛开苦降法对24例上消化道癌前病变患者舌苔菌群的影响
|
摘要
目的:基于舌苔菌群探讨辛开苦降法对有效治疗的上消化道癌前病变的微生态学机制。方法:辛开苦降法治疗上消化道癌前病变患者24例,胃镜及病理诊断进行疗效评价,基于16S r DNA高通量测序分析舌苔菌群的变化。结果:辛开苦降法治疗上消化道癌前病变的有效率为75.0%。与治疗前比较,颗粒链菌属、孪生球菌属和嗜血杆菌属丰度显著上升(P<0.05),依赖杆菌属丰度显著下降(P<0.05)。菌群共生关系显示颗粒链菌属、嗜血杆菌属和孪生球菌属间存在显著正相关(P<0.05),而颗粒链菌属、孪生球菌属、嗜血杆菌属与弯曲杆菌属存在显著负相关(P<0.05)。比较治疗前后舌苔菌群功能预测,辛开苦降法能够显著提高机体无机离子转运与代谢功能(P<0.05)。结论:舌苔菌群可为辛开苦降法有效治疗上消化道癌前病变提供潜在生物标志。
Objective: To explore the effects of pungent dispersion bitter purgation method(PDBPM) on the bacterial flora of tongue coating in patients with precancerous lesions of upper gastrointestinal(PLUG). Methods: Twenty-four cases of PLUG patients were treated by PDBPM for at least one month. Gastroscope and pathology were used to evaluate the therapeutic effect. The bacterial flora of tongue coating from the ameliorative PLUG patients was detected by 16 S r DNA next high through put sequencing. Results: Seventy-five percent of PLUG patients(18/24) treated with PDBPM were relieved by one or two grades. After PDBPM treatment, the abundance of three genera(i.e. Granulicatella, Gemella, Haemophilus) was significantly increased(P<0.05). However, the abundance of Fretibacterium was significantly decreased(P<0.05). The symbiotic relationship presented a significantly positive correlation among Granulicatella, Gemella and Haemophilus(P<0.05), while Campylobacter significantly correlated negatively to Granulicatella, Gemella, Haemophilus(P<0.05). Based on the predicted function of the bacterial flora of tongue coating, PDBPM treatment could significantly improve the function of inorganic ion transport and metabolism(P<0.05). Conclusion: The bacterial flora of tongue coating may provide potential diagnostic biomarkers of PLUG effectively treated by PDBPM.
Objective: To explore the effects of pungent dispersion bitter purgation method(PDBPM) on the bacterial flora of tongue coating in patients with precancerous lesions of upper gastrointestinal(PLUG). Methods: Twenty-four cases of PLUG patients were treated by PDBPM for at least one month. Gastroscope and pathology were used to evaluate the therapeutic effect. The bacterial flora of tongue coating from the ameliorative PLUG patients was detected by 16 S r DNA next high through put sequencing. Results: Seventy-five percent of PLUG patients(18/24) treated with PDBPM were relieved by one or two grades. After PDBPM treatment, the abundance of three genera(i.e. Granulicatella, Gemella, Haemophilus) was significantly increased(P<0.05). However, the abundance of Fretibacterium was significantly decreased(P<0.05). The symbiotic relationship presented a significantly positive correlation among Granulicatella, Gemella and Haemophilus(P<0.05), while Campylobacter significantly correlated negatively to Granulicatella, Gemella, Haemophilus(P<0.05). Based on the predicted function of the bacterial flora of tongue coating, PDBPM treatment could significantly improve the function of inorganic ion transport and metabolism(P<0.05). Conclusion: The bacterial flora of tongue coating may provide potential diagnostic biomarkers of PLUG effectively treated by PDBPM.
引文
[1]郏建臣,段秀芳.超声内镜辅助下内镜黏膜下剥离术治疗食管癌前病变的临床研究.中国内镜杂志,2015,21(4):348-351
[2]田同德,杨峰,唐静雯,等.慢性炎性反应与胃癌前病变关系及中医对策探讨.中华中医药杂志,2016,31(2):359-363
[3]房静远,刘文忠,李兆申,等.中国慢性胃炎共识意见.胃肠病学,2013,18(1):24-36
[4]杨洋,魏玮,史海霞.辛开苦降法治疗脾胃病.中医杂志,2016,57(5):446-447
[5]徐升,刘敏琪.加减半夏泻心汤治疗慢性萎缩性胃炎癌前病变临床观察.中医药临床杂志,2010,22(6):495-496
[6]王建斌.半夏泻心汤加味治疗胃癌前病变27例.西部中医药,2011,24(12):45-47
[7]理萍.半夏泻心汤治疗慢性萎缩性胃炎癌前病变临床研究.中医药临床杂志,2014,26(8):787-788
[8]冯丽丽,张爱平,董银平.半夏泻心汤在胃癌防治中的应用.中国实验方剂学杂志,2012,18(2):258-259
[9]陶丽.胃癌中医辨证分型的文献分析.中医药学刊,2006,24(4):684-686
[10]Nakano Y,Yoshimura M,Koga T.Correlation between oral malodor and periodontal bacteria.Microbes Infect,2002,4(6):679-683
[11]Zhang X,Zhang D,Jia H,et al.The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment.Nat Med,2015,21(8):895-905
[12]Zeller G,Tap J,Voigt A Y,et al.Potential of fecal microbiota for early-stage detection of colorectal cancer.Mol Syst Biol,2014,10(11):766-783
[13]Lu H,Ren Z,Li A,et al.Deep sequencing reveals microbiota dysbiosis of tongue coat in patients with liver carcinoma.Sci Rep,2016,6:33142-33152
[14]Qin J,Li Y,Cai Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes.Nature,2012,490(7418):55-60
[15]Karlsson F H,F?k F,Nookaew I,et al.Symptomatic atherosclerosis is associated with an altered gut metagenome.Nat Commun,2012,3(4):1245-1252
[16]徐欣,何金枝,周学东.口腔微生物群落在口腔与全身疾病预警中的作用.华西口腔医学杂志,2015,33(6):555-560
[17]Chen H,Jiang W.Application of high-throughput sequencing in understanding human oral microbiome related with health and disease.Front Microbiol,2014,5(508):508-513
[18]Ye J,Cai X,Yang J,et al.Bacillus as a potential diagnostic marker for yellow tongue coating.Sci Rep,2016,6:32496-32504
[19]B?rsch G,Wegener M.Campylobacter pylori-associated chronic gastritis:A new entity in gastroduodenal diseases?.Leber Magen Darm,1988,18(1):9-16
[20]林慧芝,张荫昌,张文范,等.胃幽门弯曲菌感染对胃癌发生影响的探讨.中国医科大学学报,1987,16(6):426-429
[21]Beswick E J,Suarez G,Reyes V E.Hpylori and host interactions that influence pathogenesis.World J Gastroentero,2006,12(35):5599-5605
[22]Levy M,Thaiss C A,Elinav E.Metabolites:Messengers between the microbiota and the immune system.Genes Dev,2016,30(14):1589-1597
[23]Garrett W S.Cancer and the microbiota.Sci,2015,348(6230):80-86
[24]Wroblewski L E,Peek R J,Coburn L A.The role of the microbiome in gastrointestinal cancer.Gastroenterol Clin North Am,2016,45(3):543-556
[2]田同德,杨峰,唐静雯,等.慢性炎性反应与胃癌前病变关系及中医对策探讨.中华中医药杂志,2016,31(2):359-363
[3]房静远,刘文忠,李兆申,等.中国慢性胃炎共识意见.胃肠病学,2013,18(1):24-36
[4]杨洋,魏玮,史海霞.辛开苦降法治疗脾胃病.中医杂志,2016,57(5):446-447
[5]徐升,刘敏琪.加减半夏泻心汤治疗慢性萎缩性胃炎癌前病变临床观察.中医药临床杂志,2010,22(6):495-496
[6]王建斌.半夏泻心汤加味治疗胃癌前病变27例.西部中医药,2011,24(12):45-47
[7]理萍.半夏泻心汤治疗慢性萎缩性胃炎癌前病变临床研究.中医药临床杂志,2014,26(8):787-788
[8]冯丽丽,张爱平,董银平.半夏泻心汤在胃癌防治中的应用.中国实验方剂学杂志,2012,18(2):258-259
[9]陶丽.胃癌中医辨证分型的文献分析.中医药学刊,2006,24(4):684-686
[10]Nakano Y,Yoshimura M,Koga T.Correlation between oral malodor and periodontal bacteria.Microbes Infect,2002,4(6):679-683
[11]Zhang X,Zhang D,Jia H,et al.The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment.Nat Med,2015,21(8):895-905
[12]Zeller G,Tap J,Voigt A Y,et al.Potential of fecal microbiota for early-stage detection of colorectal cancer.Mol Syst Biol,2014,10(11):766-783
[13]Lu H,Ren Z,Li A,et al.Deep sequencing reveals microbiota dysbiosis of tongue coat in patients with liver carcinoma.Sci Rep,2016,6:33142-33152
[14]Qin J,Li Y,Cai Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes.Nature,2012,490(7418):55-60
[15]Karlsson F H,F?k F,Nookaew I,et al.Symptomatic atherosclerosis is associated with an altered gut metagenome.Nat Commun,2012,3(4):1245-1252
[16]徐欣,何金枝,周学东.口腔微生物群落在口腔与全身疾病预警中的作用.华西口腔医学杂志,2015,33(6):555-560
[17]Chen H,Jiang W.Application of high-throughput sequencing in understanding human oral microbiome related with health and disease.Front Microbiol,2014,5(508):508-513
[18]Ye J,Cai X,Yang J,et al.Bacillus as a potential diagnostic marker for yellow tongue coating.Sci Rep,2016,6:32496-32504
[19]B?rsch G,Wegener M.Campylobacter pylori-associated chronic gastritis:A new entity in gastroduodenal diseases?.Leber Magen Darm,1988,18(1):9-16
[20]林慧芝,张荫昌,张文范,等.胃幽门弯曲菌感染对胃癌发生影响的探讨.中国医科大学学报,1987,16(6):426-429
[21]Beswick E J,Suarez G,Reyes V E.Hpylori and host interactions that influence pathogenesis.World J Gastroentero,2006,12(35):5599-5605
[22]Levy M,Thaiss C A,Elinav E.Metabolites:Messengers between the microbiota and the immune system.Genes Dev,2016,30(14):1589-1597
[23]Garrett W S.Cancer and the microbiota.Sci,2015,348(6230):80-86
[24]Wroblewski L E,Peek R J,Coburn L A.The role of the microbiome in gastrointestinal cancer.Gastroenterol Clin North Am,2016,45(3):543-556