桃蚜对辣椒上黄瓜花叶病毒(CMV)的传毒特性
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摘要
为预防和控制辣椒病毒病的发生,在室内采用单因素试验研究桃蚜(Myzus persicae Sulzer)对辣椒病毒[黄瓜花叶病毒(CMV)]的获毒时间、传毒时间和传毒效率。结果表明:桃蚜对辣椒病毒获毒最短时间为30s,30s后随着获毒时间延长,其取食传毒效率升高,获毒80s以上可致辣椒苗发病率达100%;桃蚜取食传毒时间最短为0.125h,之后辣椒苗发病率随着时间延长而逐渐升高,取食时间达1h以上,发病率达100%;桃蚜对辣椒病毒传毒的最少头数为1头,发病率随单株虫量的增加而升高,单株虫量为15头时辣椒苗发病率达100%。
The virus acquisition time,virus transmission time and virus transmission efficiency of Myzus persicae to Cucumber mosaic virus(CMV)of pepper were studied by the single-factor experiment indoors to prevent and control occurrence of CMV in pepper.Results:The minimum virus acquisition time of M.persicaeto CMV of pepper is 30 s.The feeding virus transmission efficiency rises with elongation of virus acquisition time and the morbidity of pepper seedlings reaches 100% when the virus acquisition time is above 80 s.The minimum feeding virus transmission time is 0.125 hand the morbidity of pepper seedlings gradually rises with elongation of feeding virus transmission time.The morbidity of pepper seedlings is up to 100% when the feeding virus transmission time is above 1 h.The minimum M.persicae number for virus transmission is 1.The morbidity of pepper seedlings rises with increase of M.persicae number per plant and the morbidity of pepper seedlings reaches 100% when the M.persicae number per plant is 15.
The virus acquisition time,virus transmission time and virus transmission efficiency of Myzus persicae to Cucumber mosaic virus(CMV)of pepper were studied by the single-factor experiment indoors to prevent and control occurrence of CMV in pepper.Results:The minimum virus acquisition time of M.persicaeto CMV of pepper is 30 s.The feeding virus transmission efficiency rises with elongation of virus acquisition time and the morbidity of pepper seedlings reaches 100% when the virus acquisition time is above 80 s.The minimum feeding virus transmission time is 0.125 hand the morbidity of pepper seedlings gradually rises with elongation of feeding virus transmission time.The morbidity of pepper seedlings is up to 100% when the feeding virus transmission time is above 1 h.The minimum M.persicae number for virus transmission is 1.The morbidity of pepper seedlings rises with increase of M.persicae number per plant and the morbidity of pepper seedlings reaches 100% when the M.persicae number per plant is 15.
引文
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[11]郑光宇.黄瓜花叶病毒新疆株的桃蚜传播特[J].吉首大学学报,2006,27(3):75-77.
[12]田兆丰,刘伟成,罗晨,等.不同蚜虫对黄瓜花叶病毒(CMV)亚组Ⅰ、Ⅱ分离物传播效率比较研究[J].华北农学报,2011,26(5):234-238.
[13]史晓斌,谢文,张友军.植物病毒病媒介昆虫的传毒特性和机制研究进展.[J].昆虫学报,2012,55(7):841-848.
[14]ZHOU X P,XIE Y,ZLUMG Z K.Molecular characterization of a distinct bcgomovirus infecting tobacco in Yannan,China[J].Arch.Viral,2001,146:1599-1606.
[2]林燕春,罗明云,林江,等.辣椒病毒病发生规律与防治技术研究[J].湖北农业科学,2009,48(9):2142-2144,2183.
[3]姚玉荣,陈国华,冯兰香,等.北运蔬菜基地辣椒病毒病病原种类的分子检测[J].中国蔬菜,2013(10):84-89.
[4]冯兰香,张宝玺,谢丙炎,等.3种辣椒新病毒的发生与血清学鉴定[J].中国蔬菜,2005(12):33-34.
[5]王海燕,张国鑫,宗峰阳,等.蚜虫传播非持久性病毒的机制研究[J].安徽农业科学,2008,36(21):9163-9164.
[6]MUKHOPADHYAY S.Plant Virus,Vector[M].New Hampshire:Science Publishers,2011.
[7]MANOUSSOPOULOS I N.Acquisition and retention of potato virus Y helper component in the transmission of potato aucuba mosaic virus by aphids[J].Journal of Phytopathology,2001,149(2):103-106.
[8]FRANZ A,MAKKOUK K M,VETTEN H J.Acquisition,retention and transmission of faba bean necrotic yellows virus by two of its aphid vectors,Aphis craccivora(Koch)and Acyrthosiphon pisum(Harris)[J].Journal of Phytopathology,1998,146(7):347-355.
[9]FIRMINO A C,YUKI V A,MOREIRA A G,et al.Tomato yellow vein streak virus:relationship with Bemisia tabaci biotype B and host range[J].Scientia Agricola,2009,66(6):793-799.
[10]SIMMONS A M,LING K S,HARRISON H F,et al.Sweet potato leaf curl virus:efficiency of acquisition,retention and transmission by Bemisia tabaci(Hemiptera:Aleyrodidae)[J].Crop Protection,2009,28:1007-1011.
[11]郑光宇.黄瓜花叶病毒新疆株的桃蚜传播特[J].吉首大学学报,2006,27(3):75-77.
[12]田兆丰,刘伟成,罗晨,等.不同蚜虫对黄瓜花叶病毒(CMV)亚组Ⅰ、Ⅱ分离物传播效率比较研究[J].华北农学报,2011,26(5):234-238.
[13]史晓斌,谢文,张友军.植物病毒病媒介昆虫的传毒特性和机制研究进展.[J].昆虫学报,2012,55(7):841-848.
[14]ZHOU X P,XIE Y,ZLUMG Z K.Molecular characterization of a distinct bcgomovirus infecting tobacco in Yannan,China[J].Arch.Viral,2001,146:1599-1606.