芥菜抗芜菁花叶病毒种质资源的鉴定与评价
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摘要
对33份不同变种的芥菜种质资源材料(大头芥4份、笋子芥1份、茎瘤芥3份、分蘖芥6份、大叶芥11份、结球芥2份、长柄芥1份、籽用芥1份、宽柄芥菜4份)进行人工机械摩擦接种,通过病情调查和抽薹期ELISA检测,最终筛选出9份抗病毒病材料。在病情调查中,17Y-7V、17Y-36V病情指数都为0,表现为免疫,其ELISA鉴定DP/DN比值小于2.1,分别为1.38、1.97,呈阴性;17Y-13V、17Y-20V、17Y-21V、17Y-31V、17Y-33V、17Y-34V、17Y-35V病情指数分别为2.86、5.33、2.00、8.00、5.93、5.71、2.00,表现为高抗,其ELISA鉴定DP/DN比值分别为1.12、0.89、1.60、0.93、0.78、0.81、1.33,均小于2.1,呈阴性。
Thirty three Brassica materials including four Brassica juncea Coss.var.megarrhiza Tsen et Lee,one B.juncea var.crassicaulis Chen et Yang,three B.juncea var.tumidaTsen et Lee,six Brassica juncea var.multicepsTsen et Lee,eleven Brassica juncea var.rugosa Bailey,two Brassica juncea var.capitata Hort,one Brassica juncea var.longepetiolata Yang et Chen,one Brassica juncea Coss.var.gracilis Tsen et Lee,four Brassica juncea var.latipaLi were inoculated with TuMV isolate.Through disease investigation and convulsion ELISA,nine mustard materials were found to be TuMVresistant,laying the foundation for disease resistance breeding.The condition indexes of both of the numbers 17 Y-7 Vand 17 Y-36 Vin disease investigation were 0,showing immunity.Its DP/DNratio of the ELISA test were less than 2.1,which were 1.38 and 1.97,showing negative performance.The condition index of the numbers 17 Y-13 V,17 Y-20 V,17 Y-21 V,17 Y-31 V,17 Y-33 V,17 Y-34 V,and 17 Y-35 Vin disease investigation were 2.86,5.33,2.00,8.00,5.93,5.71,2.00,showing high resistance.Its DP/DN ratio of the ELISA test were less than 2.1,which were 1.12,0.89,1.60,0.93,0.78,0.81,and 1.33,showing negative performance.
Thirty three Brassica materials including four Brassica juncea Coss.var.megarrhiza Tsen et Lee,one B.juncea var.crassicaulis Chen et Yang,three B.juncea var.tumidaTsen et Lee,six Brassica juncea var.multicepsTsen et Lee,eleven Brassica juncea var.rugosa Bailey,two Brassica juncea var.capitata Hort,one Brassica juncea var.longepetiolata Yang et Chen,one Brassica juncea Coss.var.gracilis Tsen et Lee,four Brassica juncea var.latipaLi were inoculated with TuMV isolate.Through disease investigation and convulsion ELISA,nine mustard materials were found to be TuMVresistant,laying the foundation for disease resistance breeding.The condition indexes of both of the numbers 17 Y-7 Vand 17 Y-36 Vin disease investigation were 0,showing immunity.Its DP/DNratio of the ELISA test were less than 2.1,which were 1.38 and 1.97,showing negative performance.The condition index of the numbers 17 Y-13 V,17 Y-20 V,17 Y-21 V,17 Y-31 V,17 Y-33 V,17 Y-34 V,and 17 Y-35 Vin disease investigation were 2.86,5.33,2.00,8.00,5.93,5.71,2.00,showing high resistance.Its DP/DN ratio of the ELISA test were less than 2.1,which were 1.12,0.89,1.60,0.93,0.78,0.81,and 1.33,showing negative performance.
引文
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[18]王雪,刘玉梅,李汉霞,等.芸薹属作物抗芜菁花叶病毒育种研究进展[J].园艺学报,2005,32(5):939-946.
[19]张成良,张作芳.酶联免疫吸附技术.Ⅲ.酶联免疫吸附法在检测植物病毒上的应用[J].植物检疫,1982(3):15-33.
[20]孙伟,焦奎.酶联免疫吸附分析法在植物病毒检测中的应用[J].化学研究与应用,2002,14(5):511-514.
[21]梁新苗,边勇,汪万春,等.ELISA在植物检疫中的应用及其质量控制[J].检验检疫学刊,2015(4):64-67.
[2]陈材林,周源,周光凡,等.中国的芥菜起源探讨[J].西南农业学报,1992,5(3):6-11.
[3]孟秋峰.芥菜类蔬菜进化及分类研究[D].杭州:浙江大学,2005.
[4]万正杰,李海渤,姚培杰,等.芥菜类蔬菜杂种优势利用的研究进展与展望[J].华中农业大学学报,2018,37(1):115-120.
[5]范永红,沈进娟,董代文.芥菜类蔬菜产业发展现状及研究前景思考[J].农学学报,2016,6(2):65-71.
[6]许智颖.中国芥菜芜菁花叶病毒抗性的分子鉴定[D].杭州:浙江大学,2006.
[7]祝富祥.芜菁花叶病毒的分子变异及其P3蛋白在病毒与寄主互作中的功能研究[D].长春:吉林大学,2016.
[8]西北农学院植保系,西安市农科所.西安地区十字花科蔬菜病毒病的鉴定[J].微生物学报,1976(2):46-51.
[9]蔡丽,许泽永,陈坤荣,等.湖北和安徽省油菜病毒病调查和病毒血清鉴定[J].植物保护,2007,33(2):88-90.
[10]张艳超,侯明生,蔡丽.湖北省蔬菜病毒病主要毒原种类检测[J].华中农业大学学报,2017,36(6):31-38.
[11]马伟伟.芥菜抗芜菁花叶病毒种质鉴定与抗性基因定位研究[D].杭州:浙江大学,2015.
[12]蔡丽.油菜病毒株系鉴定和抗病相关基因研究及转基因飘逸评价[D].武汉:华中农业大学,2008.
[13]赵建平,周钗美,陈集双,等.芜菁花叶病毒(TuMV)特性的研究进展[J].微生物学通报,2004,31(6):100-104.
[14]洪健,徐颖,黎军英,等.芜菁花叶病毒(TuMV)侵染对寄主植物光合作用的影响[J].电子显微学报,2002,21(2):110-113.
[15]潘春清,张俊华,崔崇士,等.芜菁花叶病毒研究现状[J].东北农业大学学报,2008,39(1):129-133.
[16]TOMLINSON J A.Epidemiology and control of virus diseases of vegetables.[J].Annals of applied biology,1987,110(3):661-681.
[17]TAN Z,GIBBS A J,TOMITAKA Y,et al.Mutations in Turnip mosaic virus genomes that have adapted to Raphanus sativus[J].Journal of general virology,2005,86(Pt 2):501-510.
[18]王雪,刘玉梅,李汉霞,等.芸薹属作物抗芜菁花叶病毒育种研究进展[J].园艺学报,2005,32(5):939-946.
[19]张成良,张作芳.酶联免疫吸附技术.Ⅲ.酶联免疫吸附法在检测植物病毒上的应用[J].植物检疫,1982(3):15-33.
[20]孙伟,焦奎.酶联免疫吸附分析法在植物病毒检测中的应用[J].化学研究与应用,2002,14(5):511-514.
[21]梁新苗,边勇,汪万春,等.ELISA在植物检疫中的应用及其质量控制[J].检验检疫学刊,2015(4):64-67.