不同相对分子质量的海藻酸钠与人血清白蛋白相互作用的研究
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摘要
通过加酸微波降解海藻酸钠联合凝胶色谱柱分离的方式制备了相对分子质量为3190、4897、6800、14635、20960、66500的6种不同相对分子质量的海藻酸钠。采用荧光猝灭法研究了6种不同相对分子质量的海藻酸钠与HSA的相互作用。实验结果表明,海藻酸钠与HSA存在相互作用,两者结合属于静态猝灭;热力学分析表明6种不同相对分子质量的海藻酸钠和HSA的相互作用是以氢键和范德华力为主的分子间作用。相对分子质量的变化并没影响其猝灭类型及作用力类型。结合位点数约为1。两者结合常数受相对分子质量的影响较大,在海藻酸钠相对分子质量为3190~66500之间其结合常数呈现为先增加后减小的趋势。
Through acid degradation of sodium alginate,gel chromatography column separation method preparation of 6kinds of different relative molecular weight of sodium alginate,Respectively: 3190、4897、6800、14635、20960、66500.Fluorescence quenching method is used to study the interaction of sodium alginate and HSA in this paper. Experimental results show that the interaction of Sodium alginate and HSA belongs to static quenching and combined with force as Hydrogen bonding and van der waals force. Binding points is about 1. The changes of molecular weight did not affect the quenching type and reaction type. The interaction constant was increased with molecular weight at first,but then decreased gradually between the molecular weight is 3190 ~ 66500.
Through acid degradation of sodium alginate,gel chromatography column separation method preparation of 6kinds of different relative molecular weight of sodium alginate,Respectively: 3190、4897、6800、14635、20960、66500.Fluorescence quenching method is used to study the interaction of sodium alginate and HSA in this paper. Experimental results show that the interaction of Sodium alginate and HSA belongs to static quenching and combined with force as Hydrogen bonding and van der waals force. Binding points is about 1. The changes of molecular weight did not affect the quenching type and reaction type. The interaction constant was increased with molecular weight at first,but then decreased gradually between the molecular weight is 3190 ~ 66500.
引文
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[4]赵珊.褐藻酸钠分子量检测方法的研究及其应用[D].青岛:中国海洋大学,2011.
[5]王富,徐琦,金春红,等.荧光法研究司他夫定与牛血清白蛋白的结合作用[J].浙江教育学院学报,2008(5):49-53.
[6]孙艳涛.药物小分子及其与生物大分子相互作用的光谱研究[D].吉林:吉林大学,2009.
[7]王迎进,张艳青,李亚雄,等.光谱法研究根皮苷与人血清白蛋白相互作用[J].分析测试学报,2013(2):239-243.
[8]乔春玉.药物与生物大分子相互作用的研究[D].吉林:吉林大学,2009.
[9]陈蓉蓉.光谱法研究四种青蒿素类抗疟药与人血清白蛋白的相互作用[D].广州:暨南大学,2013.
[10]Wang Z,Li D J,Jin J.Study on the interaction of puerarin with lysozyme by spectroscopic methods[J].Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy,2008,70(4):866-870.
[11]Feng X,Bai C,Lin Z,et al.Investigation of the interaction between acridine orange and bovine serum albumin[J].Talanta,1998,47(5):1223-1229.
[12]彭鑫.中药丹参活性成分与血清白蛋白的相互作用研究[D].天津:天津大学,2013.
[13]边平凤.生物大分子与小分子相互作用研究[D].杭州:浙江大学,2008.
[14]Ross P D,Subramanian S.Thermodynamics of protein association reaction;forces contribution to stability[J].Biochemistry,1981,20(11):3096-102.
[15]Tian J,Liu J,Tian X,et al.Study of the interaction of kaempferol with serum albumin[J].Journal of Molecular Structure,2004,691(1-3):197-202.