酶法辅助提取柳叶蒿挥发油的研究
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摘要
以柳叶蒿为原料,对酶法辅助提取柳叶蒿挥发油的工艺进行优化。通过单因素试验考察了纤维素酶添加量、酶解温度、酶解时间、酶解pH值、液料比等多个因素对柳叶蒿挥发油提取率的影响,并在单因素试验的基础上,采用正交试验对提取工艺条件进行了优化。结果显示,最优提取工艺条件为:加酶量100 U/g原料,酶解温度50℃,酶解时间4 h,溶液pH4.8。在此条件下,柳叶蒿经纤维素酶预处理后,利用水蒸气蒸馏法提取挥发油与未经过酶处理直接提取挥发油相比,挥发油提取率提高了0.48%。
Enzymatic extraction of volatile oil from Artemisia integrifolia was optimized. The effects of cellulase content,temperature of enzymatic hydrolysis, time of enzymatic hydrolysis, pH value of enzymatic hydrolysis and liquid-material ratio on the extraction rate of volatile oil from Artemisia integrifolia were investigated by single factor test and the orthogonal test. Results showed that the optimal extraction technology were enzyme quantity 100 U/g raw material,enzyme solution temperature 50 ℃, enzymolysis time 4 h, solution pH4.8. Under these conditions, the extraction rate of volatile oil from Artemisia integrifolia by steam distillation after cellulase pretreatment was 0.48% higher than that without enzyme treatment.
Enzymatic extraction of volatile oil from Artemisia integrifolia was optimized. The effects of cellulase content,temperature of enzymatic hydrolysis, time of enzymatic hydrolysis, pH value of enzymatic hydrolysis and liquid-material ratio on the extraction rate of volatile oil from Artemisia integrifolia were investigated by single factor test and the orthogonal test. Results showed that the optimal extraction technology were enzyme quantity 100 U/g raw material,enzyme solution temperature 50 ℃, enzymolysis time 4 h, solution pH4.8. Under these conditions, the extraction rate of volatile oil from Artemisia integrifolia by steam distillation after cellulase pretreatment was 0.48% higher than that without enzyme treatment.
引文
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[2]BORA KS,SHARMA A.The genus Artemisia:a comprehensive review[J].Pharmaceutical?biology,2011,49(1):101-109.
[3]戴小军,刘延庆,梅全喜.蒿属药用植物药理活性研究进展[J].中药材,2005,28(3):81-85.
[4]王建刚.柳蒿芽挥发性成分的SPME-GC/MS分析[J].安徽农业科学,2010,38(33):18 773-18 776.
[5]穆师洋,胡文忠,姜爱丽.柳蒿芽营养成分及生物活性成分研究进展[J].食品工业科技,2015,36(13):385-387.
[6]ZHU L.Chemical composition and antimicrobial activities of essential oil from Artemisia integrifolia[J].Asian Journal of Chemistry,2013,25(14):7 679-7 682.
[7]国家药典委员会.中华人民共和国药典(I部)[M].北京:中国医药科技出版社,2010.
[8]盛周煌,胡均鹏,朱良.柳叶蒿精油/羟丙基-β-环糊精包合物的制备与缓释抗菌性能[J].食品工业,2017,38(10):98-101.