近红外反射光谱技术测定甜玉米总多糖和淀粉含量的研究
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摘要
结合酶法测定了104份甜玉米种质总多糖和淀粉的含量,利用近红外光谱分析仪采集了相应的光谱信息,分别建立了甜玉米完整种子的总多糖和淀粉含量的近红外光谱定标模型。结果表明:最优光谱处理方式总多糖的为标准正常化+去散射处理,淀粉的为标准多元散射校正;最优的导数处理总多糖的为3阶导数,淀粉的为2阶导数。总多糖和淀粉定标模型的交互验证相关系数分别为0.680和0.734,交互验证标准偏差分别为2.537和2.406;外部验证集总多糖和淀粉的预测相关系数分别为0.834和0.783,说明所建立的总多糖和淀粉的预测模型具有较好的预测性,可应用于甜玉米种质资源筛选。
In this study, combined with total polysaccharide and starch content of 104 sweet corn germplasms measured by enzyme method, near infrared spectral calibration models for the total polysaccharide and starch content of complete sweet corn seeds were established using spectral information collected by near infrared spectrum. The results showed that the optimal spectral treatment of total polysaccharide was Standard Normalized plus Detrend and starch was Standard Multivariate Scatter Correction. The optimal derivative treatment of total polysaccharide was the 3 rd derivative, and starch was the 2 nd derivative. The correlation coefficients of calibration model of total polysaccharide and starch were 0.680 and 0.734, respectively. The standard deviations of interaction validation of total polysaccharide and starch were 2.537 and 2.406, respectively. The correlation coefficients of external sample set for verification of total polysaccharide and starch were 0.834 and 0.783, respectively. These results indicated that the model of total polysaccharide and starch had better predictability and could be applied to the selection of sweet corn germplasm resources.
In this study, combined with total polysaccharide and starch content of 104 sweet corn germplasms measured by enzyme method, near infrared spectral calibration models for the total polysaccharide and starch content of complete sweet corn seeds were established using spectral information collected by near infrared spectrum. The results showed that the optimal spectral treatment of total polysaccharide was Standard Normalized plus Detrend and starch was Standard Multivariate Scatter Correction. The optimal derivative treatment of total polysaccharide was the 3 rd derivative, and starch was the 2 nd derivative. The correlation coefficients of calibration model of total polysaccharide and starch were 0.680 and 0.734, respectively. The standard deviations of interaction validation of total polysaccharide and starch were 2.537 and 2.406, respectively. The correlation coefficients of external sample set for verification of total polysaccharide and starch were 0.834 and 0.783, respectively. These results indicated that the model of total polysaccharide and starch had better predictability and could be applied to the selection of sweet corn germplasm resources.
引文
[1] Creech R G. Genetic control of carbohydrate synthesis in maize endosperm[J]. Genetics, 1965, 52(6): 1175-1186.
[2] Garwood D L, Creech R G. Kernel phenotypes of Zea mays L. genotypes possessing one to four mutated genes1[J]. Crop Science, 1972, 12(1): 119-121.
[3] Laughnan J R. The effect of the sh2 factor on carbohydrate reserves in the mature endosperm of maize[J]. Genetics, 1953, 38(5): 485-499.
[4] 刘蔚楠,万忠,甘阳英,等.2015年广东甜玉米产业发展形势与对策建议[J].广东农业科学,2016,43(3):12-16.
[5] 赵仁贵,王逸群,蔚荣海,等.超甜玉米单交种“吉甜6号”选育报告[J].吉林农业大学学报,2007,29(3):250-252.
[6] 祁新,权继斌,姜硕,等.超甜玉米单交种“吉甜9号”选育报告[J].吉林农业大学学报,2008,30(6):774-775.
[7] Davies A M, Grant A. Review: Near infra-red analysis of food[J]. International Journal of Food Science and Technology, 2007, 22(3): 191-207.
[8] 陆婉珍.现代近红外光谱分析技术[M].2版,北京:中国石化出版社,2007:33-90.
[9] 刘小路,薛璐,鲁晓翔,等.蓝莓可溶性固形物、总酸近红外无损检测模型的建立[J].食品与生物技术学报,2016,35(7):752-756.
[10] 陈锋,何中虎,崔党群,等.利用近红外透射光谱技术测定小麦品质性状的研究[J].麦类作物学报,2003(3):1-4.
[11] 方彦,王汉宁.利用近红外光谱法测定玉米子粒含油量的研究[J].西北农业学报,2007,16(1):111-113.
[12] 魏良明,严衍禄,戴景瑞.近红外反射光谱测定玉米完整子粒蛋白质和淀粉含量的研究[J].中国农业科学,2004,37(5):630-633.
[13] 杨泉女,周权驹,王蕴波,等.甜玉米葡萄糖、果糖和蔗糖含量近红外反射光谱模型构建[J].中国农业科技导报,2018,20(1):137-146.
[14] Johnson G, Lambert C, Johnson D K, et al. Plant tissue analysis, colorimetric determination of glucose, fructose, and sucrose in plant materials using a combination of enzymatic and chemical methods[J]. Journal of Agricultural and Food Chemistry, 1964, 12(3): 216-219.
[15] Gorin N. Enzymic and high-pressure liquid chromatographic estimation of glucose, fructose, and sucrose in powders from stored onions[J]. Journal of Agricultural and Food Chemistry, 1979, 27(1): 195-197.
[16] Thavarungkul P, Suppapitnarm P, Kanatharana P, et al. Batch injection analysis for the determination of sucrose in sugar cane juice using immobilized invertase and thermometric detection[J]. Biosensors and Bioelectronics, 1999, 14(1): 19-25.
[17] Mccleary B V, Murphy A, Mugford D C, et al. Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study.[J]. Journal of AOAC International, 2000, 83(2): 356-364.
[18] Velterop J S, Vos F. A rapid and inexpensive microplate assay for the enzymatic determination of glucose, fructose, sucrose, L-malate and citrate in tomato (Lycopersicon esculentum) extracts and in orange juice[J]. Phytochemical Analysis, 2001, 12(5): 299-304.
[19] Mccleary B V, Gibson T S, Mugford D C, et al. Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study[J]. Journal of AOAC International, 1997, 80(3): 571-579.
[20] Teas H J, Newton A C. Tryptophan, niacin, and indoleacetic acid in several endosperm mutants and standard lines of maize[J]. Plant Physiology, 1951, 26(3): 494-501.
[21] 杨泉女,周权驹,吴松健,等.3,5-二硝基水杨酸法与酶法测定甜玉米还原糖和蔗糖含量的比较[J].中国农业科技导报,2017,19(11):125-131.
[2] Garwood D L, Creech R G. Kernel phenotypes of Zea mays L. genotypes possessing one to four mutated genes1[J]. Crop Science, 1972, 12(1): 119-121.
[3] Laughnan J R. The effect of the sh2 factor on carbohydrate reserves in the mature endosperm of maize[J]. Genetics, 1953, 38(5): 485-499.
[4] 刘蔚楠,万忠,甘阳英,等.2015年广东甜玉米产业发展形势与对策建议[J].广东农业科学,2016,43(3):12-16.
[5] 赵仁贵,王逸群,蔚荣海,等.超甜玉米单交种“吉甜6号”选育报告[J].吉林农业大学学报,2007,29(3):250-252.
[6] 祁新,权继斌,姜硕,等.超甜玉米单交种“吉甜9号”选育报告[J].吉林农业大学学报,2008,30(6):774-775.
[7] Davies A M, Grant A. Review: Near infra-red analysis of food[J]. International Journal of Food Science and Technology, 2007, 22(3): 191-207.
[8] 陆婉珍.现代近红外光谱分析技术[M].2版,北京:中国石化出版社,2007:33-90.
[9] 刘小路,薛璐,鲁晓翔,等.蓝莓可溶性固形物、总酸近红外无损检测模型的建立[J].食品与生物技术学报,2016,35(7):752-756.
[10] 陈锋,何中虎,崔党群,等.利用近红外透射光谱技术测定小麦品质性状的研究[J].麦类作物学报,2003(3):1-4.
[11] 方彦,王汉宁.利用近红外光谱法测定玉米子粒含油量的研究[J].西北农业学报,2007,16(1):111-113.
[12] 魏良明,严衍禄,戴景瑞.近红外反射光谱测定玉米完整子粒蛋白质和淀粉含量的研究[J].中国农业科学,2004,37(5):630-633.
[13] 杨泉女,周权驹,王蕴波,等.甜玉米葡萄糖、果糖和蔗糖含量近红外反射光谱模型构建[J].中国农业科技导报,2018,20(1):137-146.
[14] Johnson G, Lambert C, Johnson D K, et al. Plant tissue analysis, colorimetric determination of glucose, fructose, and sucrose in plant materials using a combination of enzymatic and chemical methods[J]. Journal of Agricultural and Food Chemistry, 1964, 12(3): 216-219.
[15] Gorin N. Enzymic and high-pressure liquid chromatographic estimation of glucose, fructose, and sucrose in powders from stored onions[J]. Journal of Agricultural and Food Chemistry, 1979, 27(1): 195-197.
[16] Thavarungkul P, Suppapitnarm P, Kanatharana P, et al. Batch injection analysis for the determination of sucrose in sugar cane juice using immobilized invertase and thermometric detection[J]. Biosensors and Bioelectronics, 1999, 14(1): 19-25.
[17] Mccleary B V, Murphy A, Mugford D C, et al. Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study.[J]. Journal of AOAC International, 2000, 83(2): 356-364.
[18] Velterop J S, Vos F. A rapid and inexpensive microplate assay for the enzymatic determination of glucose, fructose, sucrose, L-malate and citrate in tomato (Lycopersicon esculentum) extracts and in orange juice[J]. Phytochemical Analysis, 2001, 12(5): 299-304.
[19] Mccleary B V, Gibson T S, Mugford D C, et al. Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study[J]. Journal of AOAC International, 1997, 80(3): 571-579.
[20] Teas H J, Newton A C. Tryptophan, niacin, and indoleacetic acid in several endosperm mutants and standard lines of maize[J]. Plant Physiology, 1951, 26(3): 494-501.
[21] 杨泉女,周权驹,吴松健,等.3,5-二硝基水杨酸法与酶法测定甜玉米还原糖和蔗糖含量的比较[J].中国农业科技导报,2017,19(11):125-131.