茶树抗寒机理及其应用的研究进展
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摘要
茶树原产于热带及亚热带地区,是一种喜温畏寒的植物,北方冬季寒冷干燥,会造成茶树的冻害。因此,茶树抗寒机理的研究对北方茶树的规模种植和增产具有重要意义。基于低温胁迫下茶树的主要生理变化,从糖代谢途径、Ca~(2+)信号途径,ABA、CBF/DREB诱导抗寒基因的表达几个方面综述了茶树的抗寒机理研究进展,并介绍了抗寒机理在茶树育种、栽培中的应用。
Camellia sinensis was originated in the tropical and subtropical regions, which was suitable in warm rather than cold area. When planted in Northern China, tea plants were often usually destroyed by frozen damage in cold and dry winter. Therefore, researching the mechanism of cold resistance of Camellia sinensis was of great significance for the large-scale planting and yield increase of northern tea plant. In this research, we reviewed the main physiological changes and resistance mechanism of tea plant in low temperature with the aspects of carbohydrate metabolism pathway, Ca~(2+) signaling pathway, ABA and CBF/DREB gene expression. In addition, application of the mechanism of cold resistance in tea plant breeding and cultivation was also introduced.
Camellia sinensis was originated in the tropical and subtropical regions, which was suitable in warm rather than cold area. When planted in Northern China, tea plants were often usually destroyed by frozen damage in cold and dry winter. Therefore, researching the mechanism of cold resistance of Camellia sinensis was of great significance for the large-scale planting and yield increase of northern tea plant. In this research, we reviewed the main physiological changes and resistance mechanism of tea plant in low temperature with the aspects of carbohydrate metabolism pathway, Ca~(2+) signaling pathway, ABA and CBF/DREB gene expression. In addition, application of the mechanism of cold resistance in tea plant breeding and cultivation was also introduced.
引文
[1] KAZEMI-SHAHANDASHTI S S,MAALI-AMIRI R.Global insights of protein responses to cold stress in plants:Signaling,defence,and degradation[J].Journal of plant physiology,2018,226:123-135.
[2] SHABALA S,MANAVALAN L P,NGUYEN H T,et al.Plant stress physiology[M].Oxfordshire,UK:CABI Publishing,2017:21.
[3] 韩同春.北方茶事——“南茶北引”工程与山东茶的种植和发展[J].农业考古,2013(2):283-287.
[4] 张明欣.北方茶树安全越冬技术[J].农业科技通讯,2011(5):142,187.
[5] 姚元涛,王长君,田丽丽,等.北方茶园冻害防治方法及补救措施[J].茶叶,2011,37(2):106-107.
[6] 孙海伟,曹德航,尚涛,等.茶树抗寒育种及转基因研究进展[J].山东农业科学,2013,45(6):119-122,129.
[7] 房用,李秀芬,慕宗昭,等.茶树抗寒性研究进展[J].经济林研究,2004(2):69-72.
[8] 赵洋,刘振,杨培迪,等.茶树抗寒性研究进展及展望[J].茶叶通讯,2010,37(4):23-25.
[9] 王栋.茶树抗寒性研究进展[J].茶叶科学技术,2010(1):5-8.
[10] 邱丽玲,孙威江,陈志丹.茶树抗寒性研究进展[J].福建茶叶,2012,34(2):2-5.
[11] WANG X C,ZHAO Q Y,MA C L,et al.Global transcriptome profiles of Camellia sinensis during cold acclimation[J].BMC Genomics,2013,14(1):1-14.
[12] 林郑和,钟秋生,单睿阳,等.茶树抗寒基因CsCBF1与CsICE1低温下的表达分析[J].核农学报,2016,30(9):1693-1698.
[13] 黄玉婷.茶树CsCaMs、CsCDPKs基因家族的克隆与表达分析[D].北京:中国农业科学院,2016.
[14] 林郑和,钟秋生,陈常颂,等.低温对茶树新品系叶绿素与电导率的影响[J].福建茶叶,2014,36(5):10-11.
[15] 赵洋,刘振,杨培迪,等.茶树抗寒性研究进展及展望[J].茶叶通讯,2010,37(4):23-25.
[16] 李叶云,庞磊,陈启文,等.低温胁迫对茶树叶片生理特性的影响[J].西北农林科技大学学报(自然科学版),2012,40(4):134-138.
[17] 岳川.茶树糖类相关基因的挖掘及其在茶树冷驯化中的表达研究[D].北京:中国农业科学院,2015.
[18] 黄华涛,刘祖生,庄晚芳.茶树抗寒生理的研究:酶和细胞膜透性与茶树抗寒性[J].茶叶科学,1989,6(1):41-48.
[19] 黄建安.茶树保护性酶类与抗寒性的关系[J].茶叶科学,1990,10(1):35-40.
[20] LI N N,YUE C,CAO H L,et al.Transcriptome sequencing dissection of the mechanisms underlying differential cold sensitivity in young and mature leaves of the tea plant(Camellia sinensis)[J].Journal of plant physiology,2018,224-225:144-155.
[21] YIN Y,MA Q P,ZHU Z X,et al.Functional analysis of CsCBF3 transcription factor in tea plant(Camellia sinensis)under cold stress[J].Plant growth regulation,2016,80(3):335-343.
[22] CHEN L Q,CHEUNG L S,FENG L,et al.Transport of sugars[J].Annual review of biochemistry,2015,84(1):865-894.
[23] YUE C,CAO H L,WANG L,et al.Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season[J].Plant molecular biology,2015,88(6):591-608.
[24] SANDERS D,BROWNLEE C,HARPER J F.Communicating with calcium[J].Plant cell,1999,11(4):691-706.
[25] ZHOU R G,LI B,LIU H T,et al.Progress in the participation of Ca2+-calmodulin in heat shock signal transductio[J].Prog Nat Sci,2009,19(10):1201-1208.
[26] AL-QURAAN N A,LOCY R D,SINGH N K.Expression of calmodulin genes in wild type and calmodulin mutants of Arabidopsis thaliana under heat stress[J].Plant Physiol Biochem,2010,48:697-702.
[27] XU Y G,ROCHA P S C F,WANG M L,et al.A novel rice calmodulin-like gene,OsMSR2,enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis[J].Planta,2011,234:47-59.
[28] 黄玉婷,钱文俊,王玉春,等.茶树钙调素基因CsCaMs的克隆及其低温胁迫下的表达分析[J].植物遗传资源学报,2016,17(5):906-912.
[29] 丁杨林,施怡婷,杨淑华.植物响应低温胁迫的分子机制研究[J].生命科学,2015,27(3):398-405.
[30] XU X X,SHAO H B,MI Y Y,et al.Biotechnological implications from abscisic acid(ABA)roles in cold stress and leaf senescence as an important signal for improving plant sustainable survival under abiotic-stressed conditions [J].Critical reviews in biotechnology,2010,30(3):222-230.
[31] YUE C,CAO H L,HAO X Y,et al.Differential expression of gibberellin-and abscisic acid-related genes implies their roles in the bud activity-dormancy transition of tea plants[J].Plant cell reports,2017,37(3):425-441.
[32] WANG Y,JIANG C J,LI Y Y,et al.CsICE1 and CsCBF1:Two transcription factors involved in cold responses in Camellia sinensis[J].Plant cell reports,2012,31(1):27-34.
[33] YUE C,CAO H L,WANG L,et al.Molecular cloning and expression analysis of tea plant aquaporin(AQP)gene family.[J].Plant physiology & biochemistry,2014,83:65-76.
[34] CAO H L,WANG L,YUE C,et al.Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant(Camellia sinensis)[J].Plant physiology & biochemistry,2015,97:432-442.
[35] LI N N,QIAN W J,WANG L,et al.Isolation and expression features of hexose kinase genes under various abiotic stresses in the tea plant(Camellia sinensis)[J].Journal of plant physiology,2016,209:95-104.
[36] MARUYAMA K,SAKUMA Y,KASUGA M,et al.Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems[J].Plant journal for cell & molecular biology,2004,38(6):982-993.
[37] MARUYAMA K,TAKEDA M,KIDOKORO S,et al.Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A[J].Plant Physiol,2009,150(4):1972-1980.
[38] PARK S,LEE C M,DOHERTY C J,et al.Regulation of the Arabidopsis CBF regulon by a complex low-temperature regulatory network[J].Plant journal,2015,82(2):193-207.
[39] SEKI M,NARUSAKA M,ABE H,et al.Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray[J].Plant cell,2001,13(1):61-72.
[40] ZHAO C Z,ZHANG Z J,XIE S J,et al.Mutational evidence for the critical role of CBF transcription factors in cold acclimation in Arabidopsis[J].Plant physiology,2016,171(4):2744-2759.
[41] KIDOKORO S,YONEDA K,TAKASAKI H,et al.Different cold-signaling pathways function in the responses to rapid and gradual decreases in temperature[J].Plant cell,2017,29(4):760-774.
[42] ZHU X J,LI Q H,HU J Y,et al.Molecular cloning and characterization of spermine synthesis gene associated with cold tolerance in tea plant(Camellia sinensis)[J].Applied biochemistry & biotechnology,2015,177(5):1055-1068.
[43] WU Z J,LI X H,LIU Z W,et al.Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant(Camellia sinensis)[J].Functional & integrative genomics,2015,15(6):741-752.
[44] LI J H,ARKORFUL E,CHENG S Y,et al.Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant(Camellia sinensis(L.)O.Kuntze)[J].Scientia horticulturae,2018,238:356-362.
[45] 黄玉婷,钱文俊,王博,等.外源Ca2+及钙离子信号抑制剂对茶树抗寒性的影响[J].茶叶科学,2015,35(6):520-526.
[46] 曹红利,岳川,郝心愿,等.茶树胆碱单加氧酶CsCMO的克隆及甜菜碱合成关键基因的表达分析[J].中国农业科学,2013,46(15):3087-3096.
[47] 杨亚军,郑雷英,王新超.冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响[J].茶叶科学,2004,24(3):177-182.
[48] CHEN L,YAMAGUCHI S.RAPD markers for discriminating tea germplasms at the inter-specific level in China[J].Plant breeding,2005,124(4):404-409.
[49] HAZRA A,DASGUPTA N,SENGUPTA C,et al.Extrapolative microRNA precursor based SSR mining from tea EST database in respect to agronomic traits[J].BMC Research Notes,2017,10(1):261.
[50] HAZRA A,DASGUPTA N,SENGUPTA C,et al.Next generation crop improvement program:Progress and prospect in tea(Camellia sinensis(L.)O.Kuntze)[J].Annals of agrarian science,2018,16(2):128-135.
[2] SHABALA S,MANAVALAN L P,NGUYEN H T,et al.Plant stress physiology[M].Oxfordshire,UK:CABI Publishing,2017:21.
[3] 韩同春.北方茶事——“南茶北引”工程与山东茶的种植和发展[J].农业考古,2013(2):283-287.
[4] 张明欣.北方茶树安全越冬技术[J].农业科技通讯,2011(5):142,187.
[5] 姚元涛,王长君,田丽丽,等.北方茶园冻害防治方法及补救措施[J].茶叶,2011,37(2):106-107.
[6] 孙海伟,曹德航,尚涛,等.茶树抗寒育种及转基因研究进展[J].山东农业科学,2013,45(6):119-122,129.
[7] 房用,李秀芬,慕宗昭,等.茶树抗寒性研究进展[J].经济林研究,2004(2):69-72.
[8] 赵洋,刘振,杨培迪,等.茶树抗寒性研究进展及展望[J].茶叶通讯,2010,37(4):23-25.
[9] 王栋.茶树抗寒性研究进展[J].茶叶科学技术,2010(1):5-8.
[10] 邱丽玲,孙威江,陈志丹.茶树抗寒性研究进展[J].福建茶叶,2012,34(2):2-5.
[11] WANG X C,ZHAO Q Y,MA C L,et al.Global transcriptome profiles of Camellia sinensis during cold acclimation[J].BMC Genomics,2013,14(1):1-14.
[12] 林郑和,钟秋生,单睿阳,等.茶树抗寒基因CsCBF1与CsICE1低温下的表达分析[J].核农学报,2016,30(9):1693-1698.
[13] 黄玉婷.茶树CsCaMs、CsCDPKs基因家族的克隆与表达分析[D].北京:中国农业科学院,2016.
[14] 林郑和,钟秋生,陈常颂,等.低温对茶树新品系叶绿素与电导率的影响[J].福建茶叶,2014,36(5):10-11.
[15] 赵洋,刘振,杨培迪,等.茶树抗寒性研究进展及展望[J].茶叶通讯,2010,37(4):23-25.
[16] 李叶云,庞磊,陈启文,等.低温胁迫对茶树叶片生理特性的影响[J].西北农林科技大学学报(自然科学版),2012,40(4):134-138.
[17] 岳川.茶树糖类相关基因的挖掘及其在茶树冷驯化中的表达研究[D].北京:中国农业科学院,2015.
[18] 黄华涛,刘祖生,庄晚芳.茶树抗寒生理的研究:酶和细胞膜透性与茶树抗寒性[J].茶叶科学,1989,6(1):41-48.
[19] 黄建安.茶树保护性酶类与抗寒性的关系[J].茶叶科学,1990,10(1):35-40.
[20] LI N N,YUE C,CAO H L,et al.Transcriptome sequencing dissection of the mechanisms underlying differential cold sensitivity in young and mature leaves of the tea plant(Camellia sinensis)[J].Journal of plant physiology,2018,224-225:144-155.
[21] YIN Y,MA Q P,ZHU Z X,et al.Functional analysis of CsCBF3 transcription factor in tea plant(Camellia sinensis)under cold stress[J].Plant growth regulation,2016,80(3):335-343.
[22] CHEN L Q,CHEUNG L S,FENG L,et al.Transport of sugars[J].Annual review of biochemistry,2015,84(1):865-894.
[23] YUE C,CAO H L,WANG L,et al.Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season[J].Plant molecular biology,2015,88(6):591-608.
[24] SANDERS D,BROWNLEE C,HARPER J F.Communicating with calcium[J].Plant cell,1999,11(4):691-706.
[25] ZHOU R G,LI B,LIU H T,et al.Progress in the participation of Ca2+-calmodulin in heat shock signal transductio[J].Prog Nat Sci,2009,19(10):1201-1208.
[26] AL-QURAAN N A,LOCY R D,SINGH N K.Expression of calmodulin genes in wild type and calmodulin mutants of Arabidopsis thaliana under heat stress[J].Plant Physiol Biochem,2010,48:697-702.
[27] XU Y G,ROCHA P S C F,WANG M L,et al.A novel rice calmodulin-like gene,OsMSR2,enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis[J].Planta,2011,234:47-59.
[28] 黄玉婷,钱文俊,王玉春,等.茶树钙调素基因CsCaMs的克隆及其低温胁迫下的表达分析[J].植物遗传资源学报,2016,17(5):906-912.
[29] 丁杨林,施怡婷,杨淑华.植物响应低温胁迫的分子机制研究[J].生命科学,2015,27(3):398-405.
[30] XU X X,SHAO H B,MI Y Y,et al.Biotechnological implications from abscisic acid(ABA)roles in cold stress and leaf senescence as an important signal for improving plant sustainable survival under abiotic-stressed conditions [J].Critical reviews in biotechnology,2010,30(3):222-230.
[31] YUE C,CAO H L,HAO X Y,et al.Differential expression of gibberellin-and abscisic acid-related genes implies their roles in the bud activity-dormancy transition of tea plants[J].Plant cell reports,2017,37(3):425-441.
[32] WANG Y,JIANG C J,LI Y Y,et al.CsICE1 and CsCBF1:Two transcription factors involved in cold responses in Camellia sinensis[J].Plant cell reports,2012,31(1):27-34.
[33] YUE C,CAO H L,WANG L,et al.Molecular cloning and expression analysis of tea plant aquaporin(AQP)gene family.[J].Plant physiology & biochemistry,2014,83:65-76.
[34] CAO H L,WANG L,YUE C,et al.Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant(Camellia sinensis)[J].Plant physiology & biochemistry,2015,97:432-442.
[35] LI N N,QIAN W J,WANG L,et al.Isolation and expression features of hexose kinase genes under various abiotic stresses in the tea plant(Camellia sinensis)[J].Journal of plant physiology,2016,209:95-104.
[36] MARUYAMA K,SAKUMA Y,KASUGA M,et al.Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems[J].Plant journal for cell & molecular biology,2004,38(6):982-993.
[37] MARUYAMA K,TAKEDA M,KIDOKORO S,et al.Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A[J].Plant Physiol,2009,150(4):1972-1980.
[38] PARK S,LEE C M,DOHERTY C J,et al.Regulation of the Arabidopsis CBF regulon by a complex low-temperature regulatory network[J].Plant journal,2015,82(2):193-207.
[39] SEKI M,NARUSAKA M,ABE H,et al.Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray[J].Plant cell,2001,13(1):61-72.
[40] ZHAO C Z,ZHANG Z J,XIE S J,et al.Mutational evidence for the critical role of CBF transcription factors in cold acclimation in Arabidopsis[J].Plant physiology,2016,171(4):2744-2759.
[41] KIDOKORO S,YONEDA K,TAKASAKI H,et al.Different cold-signaling pathways function in the responses to rapid and gradual decreases in temperature[J].Plant cell,2017,29(4):760-774.
[42] ZHU X J,LI Q H,HU J Y,et al.Molecular cloning and characterization of spermine synthesis gene associated with cold tolerance in tea plant(Camellia sinensis)[J].Applied biochemistry & biotechnology,2015,177(5):1055-1068.
[43] WU Z J,LI X H,LIU Z W,et al.Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant(Camellia sinensis)[J].Functional & integrative genomics,2015,15(6):741-752.
[44] LI J H,ARKORFUL E,CHENG S Y,et al.Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant(Camellia sinensis(L.)O.Kuntze)[J].Scientia horticulturae,2018,238:356-362.
[45] 黄玉婷,钱文俊,王博,等.外源Ca2+及钙离子信号抑制剂对茶树抗寒性的影响[J].茶叶科学,2015,35(6):520-526.
[46] 曹红利,岳川,郝心愿,等.茶树胆碱单加氧酶CsCMO的克隆及甜菜碱合成关键基因的表达分析[J].中国农业科学,2013,46(15):3087-3096.
[47] 杨亚军,郑雷英,王新超.冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响[J].茶叶科学,2004,24(3):177-182.
[48] CHEN L,YAMAGUCHI S.RAPD markers for discriminating tea germplasms at the inter-specific level in China[J].Plant breeding,2005,124(4):404-409.
[49] HAZRA A,DASGUPTA N,SENGUPTA C,et al.Extrapolative microRNA precursor based SSR mining from tea EST database in respect to agronomic traits[J].BMC Research Notes,2017,10(1):261.
[50] HAZRA A,DASGUPTA N,SENGUPTA C,et al.Next generation crop improvement program:Progress and prospect in tea(Camellia sinensis(L.)O.Kuntze)[J].Annals of agrarian science,2018,16(2):128-135.