血清反复冻融对肾功能指标检测结果的影响
|
摘要
目的研究不同存储温度下(-80℃、-20℃)血清反复冻融对肾功能相关实验室指标包括尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,CREA)尿酸(uric acid,UA)检测结果的影响。方法分离胶真空采血管采集30份静脉血并分离血清进行CREA、BUN、UA的检测,检测结果作为初始水平,将检测后的血清分为2份分别于-20℃、-80℃冻存,复融后再次进行CREA、BUN、UA定量检测,如此反复进行5次,将检测结果与初始水平数据进行比较分析。结果 -80℃冻存的血清样本反复冻融2次及以上CREA、BUN、UA检测结果与初始水平相比差异均有统计学意义(P <0.05)。-20℃冻存的血清样本第2次复融后BUN检测值发生显著性改变(P <0.05),而CREA和UA较稳定,反复冻融5次后未出现明显的升高或降低。结论反复冻融对血清中肾功能指标检测结果会产生较大影响,低温(-80℃、-20℃)冻存时的血清样本应进行分装,冷冻的血清样本应该解冻一次以保持最佳的性能;本实验有利于指导样本的保存和复查工作,提示临床检验者和科研工作者应避免反复冻融对检测结果的影响,以保证实验结果的准确性。
Objective To identify the effect of repeated freezing and thawing on the serum concentrations of laboratory indicators of renal function, including blood urea nitrogen(BUN), creatinine( CREA), and uric acid( UA) at different temperatures(80℃,20℃). Methods We assessed the concentration changes of CREA,BUN,and UA in 30 mixed serum samples subjected to 1-5 freeze-thaw cycles under-80℃ and-20℃,respectively, and the results were compared with values of control samples without freeze-thawing cycle(baseline). Results At-80℃, blood urea nitrogen(BUN), creatinine(CREA), and uric acid(UA) exposed to two freeze-thaw cycles were significantly different from the baseline level(P < 0. 05). At-20℃, BUN exposed to two freeze-thaw cycles were changed significantly(P < 0.05), while repeated freezing and thawing by up to five cycles did not affect the serum concentrations of CREA and UA. Conclusion Our study shows that the serum concentrations of laboratory indicators of renal function are affected in both-80℃ and-20℃ by repeated freezing and thawing. Frozen serum should only be thawed one time to keep its best capability. The research we did is beneficial to guide the specimen preservation and reexamine and should be taken into account in the process of storage and assaying the blood samples.
Objective To identify the effect of repeated freezing and thawing on the serum concentrations of laboratory indicators of renal function, including blood urea nitrogen(BUN), creatinine( CREA), and uric acid( UA) at different temperatures(80℃,20℃). Methods We assessed the concentration changes of CREA,BUN,and UA in 30 mixed serum samples subjected to 1-5 freeze-thaw cycles under-80℃ and-20℃,respectively, and the results were compared with values of control samples without freeze-thawing cycle(baseline). Results At-80℃, blood urea nitrogen(BUN), creatinine(CREA), and uric acid(UA) exposed to two freeze-thaw cycles were significantly different from the baseline level(P < 0. 05). At-20℃, BUN exposed to two freeze-thaw cycles were changed significantly(P < 0.05), while repeated freezing and thawing by up to five cycles did not affect the serum concentrations of CREA and UA. Conclusion Our study shows that the serum concentrations of laboratory indicators of renal function are affected in both-80℃ and-20℃ by repeated freezing and thawing. Frozen serum should only be thawed one time to keep its best capability. The research we did is beneficial to guide the specimen preservation and reexamine and should be taken into account in the process of storage and assaying the blood samples.
引文
[1]SIMUNDIC A M, LIPPI G. Preanalytical phase--a continuouschallenge for laboratory professionals[J]. Biochem Med(Zagreb),2012,22(2):145-149.
[2]中华人民共和国国家卫生和计划生育委员会.临床实验室质量指标[S],WST/T496-2017.2017.
[3]KIFT R L,BYRNE C,LIVERSIDGE R,et al. The effect of storage conditions on sample stability in the routine clinical laboratory[J].Ann Clin Biochem,2015,52(Pt 6):675-679.
[4]ISMAIL A A. Identifying and reducing potentially wrong immunoassay results even when plausible and “not-unreasonable”[J]. Adv Clin Chem,2014,66:241-293.
[5]WOLTHUIS A. Impact of disease on interferences in blood bioanalysis[J]. Bioanalysis,2011,3(19):2223-2231.
[6]赵万霞,王何婷,任月秋,等.糖尿病肾病早期标志物研究进展[J].国际内分泌代谢杂志,2018,38(3):192-195.
[7]梁敏,侯凡凡.急性肾损伤生物标志物的研究进展[J].临床肾脏病杂志,2015,15(1):4-6.
[8]LUYCKX V A,TONELLI M,STANIFER J W. The global burden of kidney disease and the sustainable development goals[J]. Bull World Health Organ,2018,96(6):414-422.
[9]MWASONGWE S E,FULOP T,KATZ R,et al. Relation of uric acid level to rapid kidney function decline and development of kidney disease:The Jackson Heart Study[J]. J Clin Hypertens,2018,20(4):775-783.
[10]MACEDO E. Blood urea Nitrogen beyond estimation of renal function[J]. Crit Care Med,2011,39(2):405-406.
[11]宋雪,史尊基.糖尿病患者肾功能生物标志物检测和认知损害之间的联系[J].国际检验医学杂志,2016,37(11):1532-1535.
[12]GISLEFOSS R E, LAURITZEN M, LANGSETH H A. Effect of multiple freeze-thaw cycles on selected biochemical serum components[J]. Clin Chem Lab Med,2017,55(7):67-973.
[13]康熙雄,杨晓林,张国军,等.发光免疫分析技术临床应用手册[M].北京:高等教育出版社,2010:174-175.
[14]司学众,卜颖,张国军,等.血清样本反复冻融对甲状腺功能相关实验室指标检测结果的影响[J].中国卫生检验杂志,2010,20(10):2524-2525.
[15]CUHADAR S,KOSEOGLU M,ATAY A,et al. The effect of storage time and freeze-thaw cycles on the stability of serum samples[J].Biochem Med(Zagreb),2013,23(1):70-77.
[16]HILLEBRAND J J, HEIJBOER A C, ENDERT E. Effects of repeated freeze-thaw cycles on endocrine parameters in plasma and serum[J]. Ann Clin Biochem,2017,54(2):289-292.
[2]中华人民共和国国家卫生和计划生育委员会.临床实验室质量指标[S],WST/T496-2017.2017.
[3]KIFT R L,BYRNE C,LIVERSIDGE R,et al. The effect of storage conditions on sample stability in the routine clinical laboratory[J].Ann Clin Biochem,2015,52(Pt 6):675-679.
[4]ISMAIL A A. Identifying and reducing potentially wrong immunoassay results even when plausible and “not-unreasonable”[J]. Adv Clin Chem,2014,66:241-293.
[5]WOLTHUIS A. Impact of disease on interferences in blood bioanalysis[J]. Bioanalysis,2011,3(19):2223-2231.
[6]赵万霞,王何婷,任月秋,等.糖尿病肾病早期标志物研究进展[J].国际内分泌代谢杂志,2018,38(3):192-195.
[7]梁敏,侯凡凡.急性肾损伤生物标志物的研究进展[J].临床肾脏病杂志,2015,15(1):4-6.
[8]LUYCKX V A,TONELLI M,STANIFER J W. The global burden of kidney disease and the sustainable development goals[J]. Bull World Health Organ,2018,96(6):414-422.
[9]MWASONGWE S E,FULOP T,KATZ R,et al. Relation of uric acid level to rapid kidney function decline and development of kidney disease:The Jackson Heart Study[J]. J Clin Hypertens,2018,20(4):775-783.
[10]MACEDO E. Blood urea Nitrogen beyond estimation of renal function[J]. Crit Care Med,2011,39(2):405-406.
[11]宋雪,史尊基.糖尿病患者肾功能生物标志物检测和认知损害之间的联系[J].国际检验医学杂志,2016,37(11):1532-1535.
[12]GISLEFOSS R E, LAURITZEN M, LANGSETH H A. Effect of multiple freeze-thaw cycles on selected biochemical serum components[J]. Clin Chem Lab Med,2017,55(7):67-973.
[13]康熙雄,杨晓林,张国军,等.发光免疫分析技术临床应用手册[M].北京:高等教育出版社,2010:174-175.
[14]司学众,卜颖,张国军,等.血清样本反复冻融对甲状腺功能相关实验室指标检测结果的影响[J].中国卫生检验杂志,2010,20(10):2524-2525.
[15]CUHADAR S,KOSEOGLU M,ATAY A,et al. The effect of storage time and freeze-thaw cycles on the stability of serum samples[J].Biochem Med(Zagreb),2013,23(1):70-77.
[16]HILLEBRAND J J, HEIJBOER A C, ENDERT E. Effects of repeated freeze-thaw cycles on endocrine parameters in plasma and serum[J]. Ann Clin Biochem,2017,54(2):289-292.