三白草药材HPLC指纹图谱研究
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摘要
目的建立三白草药材的HPLC指纹图谱分析方法,为有效控制其质量提供可靠的依据。方法采用HPLC法,色谱柱为Waters Xbridge~(TM) C_(18)(4.6 mm×250 mm,5μm),流动相为甲醇-H_2O梯度洗脱;流速为1.0 mL/min;检测波长为238 nm,柱温为25℃,进样量为10μL。采用中药色谱指纹图谱相似度评价系统(2004A)和SPSS 21.0统计软件进行相似度评价和聚类分析。结果建立了不同产地的三白草药材的HPLC指纹图谱,确定了20个共有色谱峰为特征峰,并指认芦丁、金丝桃苷、槲皮苷、马兜铃内酰胺AⅡ、1′-表-三白草酮、(-)-三白草醇甲基醚、三白草酮、三白草醇、saurucinolⅠ、里卡灵A、4-O-去甲基马纳萨亭B、里卡灵B、马纳萨亭B共13个共有峰,15批三白草药材指纹图谱与对照指纹图谱相似度>0.90,整体相似度较好,聚类结果与相似度评价结果基本一致。结论所建立的指纹图谱及分析模式稳定可靠,可为三白草药材的质量控制提供更全面的参考。
Objective To establish an HPLC method of fingerprint analysis on Saururus chinensis(Lour.) Baill. for the quality control. Methods The chromatographic fingerprints were obtained by the Waters Xbridge C_(18) column(4. 6 mm ×250 mm,5 μm) with the gradient elution solvent system composed of methanol and water. The wavelength was set at 238 nm,the column temperature was 25 ℃,the flow rate was 1.0 mL/min and the injection volume was 10 μL. The HPLC fingerprint chromatograms were compared for similarity by using "Traditional Chinese Medicine chromatographic fingerprint similarity evaluation system"(2004 A) and issued by Chinese pharmacopoeia commission and cluster analysis in IBM SPSS Statistic 21. Results Twenty main common peaks were selected in the standard fingerprint and thirteen peaks of those were identified as rutin,hyperoside,quercitrin,aristololactam A Ⅱ,1′-epi-saurucinone,saurucinol methyl ether,saurucinone,saucerneol,saurucinolⅠ,likarin A,4-O-demethylmanassantin B,licarin B,manassantin B. There were fifteen samples fingerprints with good similarities with correlation coefficients higher than 0. 90 compared with the standard fingerprint. The overall similarity was good,and the cluster analysis result was basically the same as the similarity evaluation result. Conclusion The method with good reproducibility is stable and reliable,which can be applied for more comprehensive quality assessment for Saururus chinensis(Lour.) Baill.
Objective To establish an HPLC method of fingerprint analysis on Saururus chinensis(Lour.) Baill. for the quality control. Methods The chromatographic fingerprints were obtained by the Waters Xbridge C_(18) column(4. 6 mm ×250 mm,5 μm) with the gradient elution solvent system composed of methanol and water. The wavelength was set at 238 nm,the column temperature was 25 ℃,the flow rate was 1.0 mL/min and the injection volume was 10 μL. The HPLC fingerprint chromatograms were compared for similarity by using "Traditional Chinese Medicine chromatographic fingerprint similarity evaluation system"(2004 A) and issued by Chinese pharmacopoeia commission and cluster analysis in IBM SPSS Statistic 21. Results Twenty main common peaks were selected in the standard fingerprint and thirteen peaks of those were identified as rutin,hyperoside,quercitrin,aristololactam A Ⅱ,1′-epi-saurucinone,saurucinol methyl ether,saurucinone,saucerneol,saurucinolⅠ,likarin A,4-O-demethylmanassantin B,licarin B,manassantin B. There were fifteen samples fingerprints with good similarities with correlation coefficients higher than 0. 90 compared with the standard fingerprint. The overall similarity was good,and the cluster analysis result was basically the same as the similarity evaluation result. Conclusion The method with good reproducibility is stable and reliable,which can be applied for more comprehensive quality assessment for Saururus chinensis(Lour.) Baill.
引文
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[9] 陈宏降,陈建伟,李祥.三白草不同药用部位HPLC抗炎药效指纹图谱研究[J].中药材,2017,40(9):2116-2120.
[2] 肖伟,彭冰,彭勇.三白草的研究进展[J].中草药,2010,41(12):2111-2115.
[3] 李泽友,陈峰,任守忠,等.三白草的化学成分和药理作用研究进展[J].中国药房,2007,18(6):473-474.
[4] 郭凌霄.三白草总黄酮的提取及抗氧化活性研究[J].齐齐哈尔医学院学报,2010,31(14):2192-2194.
[5] 陈宏降,李祥,陈建伟,等.中药三白草地上部位的化学成分研究(Ⅰ)[J].南京中医药大学学报,2009,25(4):286-288.
[6] 王晓燕,黄霞,王海波.HPLC测定三白草不同药用部位三白草酮的含量[J].药物分析杂志,2015,35(8):1505-1508.
[7] 陈宏降,李祥,陈建伟.三白草的质量标准研究[J].中草药,2010,46(10):997-999.
[8] 王隶书,赵大庆,程东岩,等.HPLC法测定不同产地三白草三白草酮的含量[J].中国药师,2008,11(3):283-284.
[9] 陈宏降,陈建伟,李祥.三白草不同药用部位HPLC抗炎药效指纹图谱研究[J].中药材,2017,40(9):2116-2120.