伪狂犬病病毒GD1406变异株的抗原性分析
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摘要
为了解近年来广东省伪狂犬病病毒(PRV)变异情况,本研究从猪场收集Bartha-K61活疫苗免疫后的猪血清559份,经ELISA筛选出326份gB抗体阳性且gE抗体阴性的猪血清,进行中和试验,分析血清中的疫苗抗体对Bartha-K61株和临床分离到的PRV GD1406野毒株的中和能力。进一步应用小鼠进行交叉免疫保护性试验。结果显示,187份gB阳性且gE阴性的猪血清样品对Bartha-K61株和PRV GD1406野毒株的平均中和抗体滴度分别为1:57和1:13,并且免疫Bartha-K61株使小鼠免受PRV GD1406野毒株致死性攻击的保护率仅为20%,而免疫PRV GD1406野毒株使小鼠免受Bartha-K61株致死性攻击的保护率为100%,免疫灭活PRV GD1406野毒株使小鼠免受PRV GD1406野毒株致死性攻击的保护率为40%。根据试验结果推测,PRV GD1406野毒株与Batha-K61株之间存在抗原差异性,现用疫苗不能有效抵抗PRV变异株攻击。本研究结果将为PRV的防控提供实验依据,为研制PRV新疫苗提供新的思路。
To investigate the variation of pseudorabies virus(PRV) in Guangdong from 2011, a total of 559 vaccinated serum smples were collected from several pig farms to screen out the 326 g B-positive and g E-negative sera by ELISA. Neutralizing titers against Bartha-K61 and the PRV GD1406 strain were determined using cross-immunoprotection test in mice. The neutralization test results showed that average neutralizing titers of these 187 samples against Bartha-K61 and PRV GD1406 were 1 ∶57 and 1∶13,respectively. Bartha-K61 immunization was less effective against PRV GD1406 challenge, the survival rate of mice was 1/5. In contrast, PRV GD1406 immunization was effective against Bartha-K61 challenge, and provided completely protection for the mice.Inactivated PRV GD1406 immunization was less effective against PRV GD1406 challenge, and the survival rate of mice was 2/5.In conclusion, it is speculat that the PRV GD1406 has antigenic alterations compared with Bartha-K61 strain and conventional vaccines are less effective against new emergent PRVs challenge. The present study provides basis for the prevention and control of PRV and new ideas for the vaccine development.
To investigate the variation of pseudorabies virus(PRV) in Guangdong from 2011, a total of 559 vaccinated serum smples were collected from several pig farms to screen out the 326 g B-positive and g E-negative sera by ELISA. Neutralizing titers against Bartha-K61 and the PRV GD1406 strain were determined using cross-immunoprotection test in mice. The neutralization test results showed that average neutralizing titers of these 187 samples against Bartha-K61 and PRV GD1406 were 1 ∶57 and 1∶13,respectively. Bartha-K61 immunization was less effective against PRV GD1406 challenge, the survival rate of mice was 1/5. In contrast, PRV GD1406 immunization was effective against Bartha-K61 challenge, and provided completely protection for the mice.Inactivated PRV GD1406 immunization was less effective against PRV GD1406 challenge, and the survival rate of mice was 2/5.In conclusion, it is speculat that the PRV GD1406 has antigenic alterations compared with Bartha-K61 strain and conventional vaccines are less effective against new emergent PRVs challenge. The present study provides basis for the prevention and control of PRV and new ideas for the vaccine development.
引文
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[2]Wu Rui,Bai Chao-yong,Sun Jin-zhong,et al.Emergence of virulent pseudorabies virus infection in Northern China[J].JVet Sci,2013,14(3):363.
[3]Yu Xiu-ling,Zhou Zhi,Hu Dong-mei,et al.Pathogenic pseudorabies virus,China,2012[J].Emerg Infect Dis,2014.20(1):102-104.
[4]袁红,练斯南,张衡,等.新型猪伪狂犬病流行情况及净化措施[J].中国动物检疫,2016,33(3):58-62.
[5]Yu Xiu-ling,Zhou Zhi,Hu Dong-mei,et al.Pathogenic pseudorabies virus,China,2012[J].Emerg Infect Dis,2014,20(1):102-104.
[6]范克伟,杨小燕,杨守深,等.闽西地区猪伪狂犬病病毒变异株gE基因新变异序列鉴定[J].中国兽医学报,2015,35(12):1903-1909.
[7]Zhou Jin-zhu,Li Shuang,Wang Xiao-bo,et al.Bartha-K61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus[J].Vaccine,2017,35(8):1161-1166.
[8]周春陵,谭鑫,许拓,等.PRV变异株的分离及抗原差异性分析[J].中国兽医学报,2017,37(3):404-409.
[9]范克伟,戴爱玲,吴德峰,等.猪伪狂犬病病毒病变异株(Fujian-LY)对免疫仔猪的致病性研究[J].中国兽医学报,2015,35(11):1727-1734.
[10]王凤求,何永龙,侯月娥,等.广东一株猪伪狂犬病毒野毒的分离鉴定和致病性初步研究[J].黑龙江畜牧兽医,2018,(1):144-147.
[11]殷震,刘景华.动物病毒学(第2版)[M].北京:科学出版社,1997.
[12]Yu Xiu-ling,Zhou Zhi,Hu Dong-mei,et al.Pathogenic pseudorabies virus,China,2012[J].Emerg Infect Dis,2014,20(1):102-104.
[13]彭金美,安同庆,赵鸿远,等.猪伪狂犬病病毒新流行株的分离鉴定及抗原差异性分析[J].中国预防兽医学报,2013,35(1):1-4.
[14]An Tong-qing,Peng Jin-mei,Tian Zhi-jun,et al.Pseudorabies virus variant in Bartha-K61-vaccinated pigs,China,2012[J].Emerg Infect Dis,2013,19(11):1749-1755.
[15]余腾.猪伪狂犬病病毒(HNX株)的生物学特性与比较基因组学研究[D].武汉:华中农业大学,2016.
[16]Wang Chun-hua,Yuan Jin,Qin Hua-yang,et al.A novel gE-deleted pseudorabies virus(PRV)provides rapid and complete protection from lethal chalenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China[J].Vaccine,2014,32(27):3379-3385.
[17]Liang Chao,Tong Wu,Zheng Hao,et al.A high-temperature passaging attenuated Pseudorabies vaccine protects piglets completely against emerging PRV variant[J].Res Vet Sci,2017,112:109-115.
[18]童光志,陈焕春.伪狂犬病流行现状及我国应采取的防制措施[J].中国兽医学报,1999,(01):4-5.