四种药剂柱头处理对芍药属远缘杂交授粉结实率的影响
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摘要
为提高芍药属远缘杂交结实率,对3类5个远缘杂交组合进行柱头处理,即:1)牡丹组革质花盘亚组内杂交:‘凤丹白’ב芳纪’,紫斑牡丹ב雪映桃花’;2)革质花盘亚组和肉质花盘亚组杂交:紫牡丹ב日月锦’,卵叶牡丹×黄牡丹;3)牡丹组和芍药组间杂交:紫斑牡丹ב红艳争辉’。各组合采用4种处理方式:2.0%,3.5%,5.0%KCL溶液处理柱头;1.5%,3.0%,4.5%NaCl溶液处理柱头;25、50和75mg/L GA3处理柱头;花粉培养液处理柱头,比较不同授粉方式对结实率的影响。结果表明:1)KCL溶液效果优于NaCl溶液,3.5%KCl效果最好;2)所有组合结实率最高值均出现在GA3处理中,GA325mg/L最为稳妥;3)最佳培养液配方为蔗糖100g/L+H3BO30.08g/L+CaCl220mg/L,但花粉培养液处理仅对革质花盘亚组内杂交组合‘凤丹白’ב芳纪’组合有促进作用。
To enhance the compatibility of distant hybridization for improving breeding efficiency,5 cross combinations of 3 types were set:1)Leathery flower disc subgroups hybridization:Paeonia ostii‘Fengdan Bai'×P.suffruticosa‘Hoki',P.rockii× P.suffruticosa ‘XueyingTaohua';2)Intersubsectional hybridization:P.delavayi× P.suffruticosa‘JitsugetsuNishiki',P.qiui×P.lutea;3)Intersectional hybridization:P.rockii×P.lactiflora‘HongyanZhenghui'.Each combination was processed in four treatments:KCl solution treatment(2.0%,3.5% and5.0% KC,respectively),NaCl solution treatment(1.5%,3.0% and 4.5% NaCl respectively),GA3 treatment(25,50 and 75 mg/L GA3,respectively).The stigmas were treated with these pollen culture solutions to compare their effects on seed-setting rate.The result showed that:1)The effect of KCl solution treatment was better than that of NaCl solution,and the concentration of 3.5% KCl worked the best;2)The seed-setting rate in each combination all reached the highest in the GA3 treatments,and the 25 mg/L GA3 treatment was the most appropriate;3)The optimum pollen culture solution was:Sucrose 100 g/L+ H3 BO30.08 g/L+CaCl210 mg/L,but it was only effective for Paeonia ostii‘Fengdan Bai'×P.suffruticosa‘Hoki cross combination.
To enhance the compatibility of distant hybridization for improving breeding efficiency,5 cross combinations of 3 types were set:1)Leathery flower disc subgroups hybridization:Paeonia ostii‘Fengdan Bai'×P.suffruticosa‘Hoki',P.rockii× P.suffruticosa ‘XueyingTaohua';2)Intersubsectional hybridization:P.delavayi× P.suffruticosa‘JitsugetsuNishiki',P.qiui×P.lutea;3)Intersectional hybridization:P.rockii×P.lactiflora‘HongyanZhenghui'.Each combination was processed in four treatments:KCl solution treatment(2.0%,3.5% and5.0% KC,respectively),NaCl solution treatment(1.5%,3.0% and 4.5% NaCl respectively),GA3 treatment(25,50 and 75 mg/L GA3,respectively).The stigmas were treated with these pollen culture solutions to compare their effects on seed-setting rate.The result showed that:1)The effect of KCl solution treatment was better than that of NaCl solution,and the concentration of 3.5% KCl worked the best;2)The seed-setting rate in each combination all reached the highest in the GA3 treatments,and the 25 mg/L GA3 treatment was the most appropriate;3)The optimum pollen culture solution was:Sucrose 100 g/L+ H3 BO30.08 g/L+CaCl210 mg/L,but it was only effective for Paeonia ostii‘Fengdan Bai'×P.suffruticosa‘Hoki cross combination.
引文
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[9]娄琦.鸢尾种间杂交亲和性及胚胎学研究[D].沈阳:沈阳农业大学,2011Lou Q.Study on cross-compatibility and embryology of interspecific hybridization in Iris[D].Shenyang:Shenyang Agricultural University,2011(in Chinese)
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[11]何桂梅.牡丹远缘杂交育种及其胚培养与体细胞胚发生的研究[D].北京:北京林业大学,2006He G M.Studies on distant cross-breeding and embryo in vitro culture and somatic embryogensis in tree peonies[D].Beijing:Beijing Forestry University,2006(in Chinese)
[12]赵娜.牡丹远缘杂交后代及亲本形态与核型研究[D].北京:北京林业大学,2014Zhao N.Study on morphology and chromosome karyotype of distant hybridization progenies and their parents of tree peony[D].Beijing:Beijing Forestry University,2014(in Chinese)
[13]杨晓苓,杨利平,尚爱芹,刘凤栾.不同授粉处理对百合花粉萌发生长及坐果率的影响[J].河北农业大学学报,2009,32(2):46-49Yang X L,Yang L P,Shang A Q,Liu F L.Effect of different cross-pollination on pollen tube germination and fruit set of lily[J].Journal of Agricultural University of Hebei,2009,32(2):46-49(in Chinese)
[14]马莉.百合属杂交育种的研究[D].北京:北京林业大学,2008Ma L.Research of Cross-breeding of Lilium[D].Beijing:Beijing Forestry University,2008(in Chinese)
[15] Robert W,Pryor L D.Intersepecific hrbridisation in Polar[J].Theoretical and Applied Genetics,1976,47:141-151
[16]陈越.NaCl溶液可克服白菜型油菜自交不亲和性[J].陕西农业科学,1997,(3):43-44Chen Y.NaCl solution can overcome self incompatibility of Brassica napus[J].Agricultural Science of Shanxi,1997,(3):43-44(in Chinese)
[17]刘凤栾.克服百合远缘杂交不亲和性的研究[D].保定:河北农业大学,2009Liu F L. Research of overcoming cross-incompatibility between distantly related species of Lilium[D].Baoding:Hebei Agricultural University,2000(in Chinese)
[18] Myodo H.Experimental studies on the sterility of some Lilium species[J].Plant Science,1962,52(1):70-122
[19]杨晓苓.授粉方式对百合亲和性的影响[D].保定:河北农业大学,2009Yang X L.The influence of different pollinations on the compatibility in Lilium[D].Baoding:Hebei Agricultural University,2009(in Chinese)
[20] Vantuyl J M,Vandien M P. Application of in vitro pollination,ovary culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses[J].Plant Science,1991,74:115-126
[21]李文钿,马丰山.木本植物有性杂交生殖生物学图谱[M].北京:科学出版社,2011Li W D,Ma F S.Map of Reproductive Biology of Woody Plants in Sexual Hybridization[M].Beijing:Science Press,2011(in Chinese)
[22] Shin H,Akira T.Incompatible pollen tube growth and protein composition in styles of Japanese pear following high temperature treatments[J].Euphyical,1989,43:191-196
[23]李玉花,邓明琴,景士西,刘福全.激光和高压静电场对草莓种间杂交不亲和性的影响[J].激光生物学,1995,4(2):636-641Li Y H,Deng M Q,Jiang S X,Liu F Q.The influence of laser and high-voltage electrostatic field on incompatibility of strawberry interspecific hybridization[J].Laster Biology,1995,4(2):636-641(in Chinese)
[24] Gaget M, Villar M, Dumas C. The mentor pollen phenomenon in poplars:A new concept[J].Theortical and Appi lied Genetles,1989,78:129-135
[2]侯祥云,郭先锋.芍药属植物杂交育种研究进展[J].园艺学报,2013,40(9):1805-1812Hou X Y,Guo X F.Progress in paeonia crossbreeding research[J].Acta Horticulturae Sinica,2013,40(9):1805-1812(in Chinese)
[3]刘改秀.牡丹与芍药远缘杂交育种技术[J].现代农业科技,2017(2):125-126Liu G X.Cross breeding technology of tree peony and peony[J].Modern Agricultural Science and Technology,2017(2):125-126(in Chinese)
[4] Qing H,Noriakai A,Jyuoko K,Tetsuya K,Kyeong S C,Yusuke A,Nobuo K.Crossibility of Amercian tree peony‘Hign Noon’as seed parent with Japanese cultivars to breed superior cultivars[J].Euphytica,2013,191:35-44
[5]韩丽晓.芍药属杂交育种及杂交后代鉴定的研究[D].北京:北京林业大学,2014Han L X.Studies on the hybrid breeding of Paeonia and identificationofhybrids[D]. Beijing:BeijingForestry University,2014(in Chinese)
[6]郝津藜,赵娜,石颜通,王福,袁涛,王莲英.黄牡丹远缘杂交亲和性及杂交后代形态分析[J].园艺学报,2014,41(8):1651-1662Hao J L,Zhao N,Shi Y T,Wang F,Yuan T,Wang L Y.Distant hybridization compatibility of paeonia lutea and morphological analysis of its progenies[J].Acta Horticulturae Sinica,2014,41(8):1651-1662(in Chinese)
[7]刘欣.卵叶牡丹杂交育种初步研究[C]//中国观赏园艺研究进展论文集2016.北京:中国园艺学会观赏园艺专业委员会,国家花卉工程技术研究中心,2016:4Liu X.Preliminary exploration on hybridization of Paeonia qiui[C] In:Proceedings of Advances in Ornamental Horticulture of China Annual Conference 2016.Beijing:China HorticulturalSocietyornamentalgardeningSpecialized Committee, NationalFlowerEngineeringTechnology Research Center,2016:4(in Chinese)
[8]翟红,刘庆昌.用2,4-D处理获得可育甘薯组种间杂种[J].农业生物技术学报,2002,10(2):113-115Zhai H,Liu Q C.Production of fertile interspecific hybrids of ipomoea section batatas by 2,4-D treatment[J].Journal of Agricultural Biotechnology,2002,10(2):113-115(in Chinese)
[9]娄琦.鸢尾种间杂交亲和性及胚胎学研究[D].沈阳:沈阳农业大学,2011Lou Q.Study on cross-compatibility and embryology of interspecific hybridization in Iris[D].Shenyang:Shenyang Agricultural University,2011(in Chinese)
[10]刘凤栾.克服百合远缘杂交不亲和性的研究[D].保定:河北农业大学,2009Liu F L. Research of overcoming cross-incompatibility between distantly related species of Lilium[D].Baoding:Hebei Agricultural University,2009(in Chinese)
[11]何桂梅.牡丹远缘杂交育种及其胚培养与体细胞胚发生的研究[D].北京:北京林业大学,2006He G M.Studies on distant cross-breeding and embryo in vitro culture and somatic embryogensis in tree peonies[D].Beijing:Beijing Forestry University,2006(in Chinese)
[12]赵娜.牡丹远缘杂交后代及亲本形态与核型研究[D].北京:北京林业大学,2014Zhao N.Study on morphology and chromosome karyotype of distant hybridization progenies and their parents of tree peony[D].Beijing:Beijing Forestry University,2014(in Chinese)
[13]杨晓苓,杨利平,尚爱芹,刘凤栾.不同授粉处理对百合花粉萌发生长及坐果率的影响[J].河北农业大学学报,2009,32(2):46-49Yang X L,Yang L P,Shang A Q,Liu F L.Effect of different cross-pollination on pollen tube germination and fruit set of lily[J].Journal of Agricultural University of Hebei,2009,32(2):46-49(in Chinese)
[14]马莉.百合属杂交育种的研究[D].北京:北京林业大学,2008Ma L.Research of Cross-breeding of Lilium[D].Beijing:Beijing Forestry University,2008(in Chinese)
[15] Robert W,Pryor L D.Intersepecific hrbridisation in Polar[J].Theoretical and Applied Genetics,1976,47:141-151
[16]陈越.NaCl溶液可克服白菜型油菜自交不亲和性[J].陕西农业科学,1997,(3):43-44Chen Y.NaCl solution can overcome self incompatibility of Brassica napus[J].Agricultural Science of Shanxi,1997,(3):43-44(in Chinese)
[17]刘凤栾.克服百合远缘杂交不亲和性的研究[D].保定:河北农业大学,2009Liu F L. Research of overcoming cross-incompatibility between distantly related species of Lilium[D].Baoding:Hebei Agricultural University,2000(in Chinese)
[18] Myodo H.Experimental studies on the sterility of some Lilium species[J].Plant Science,1962,52(1):70-122
[19]杨晓苓.授粉方式对百合亲和性的影响[D].保定:河北农业大学,2009Yang X L.The influence of different pollinations on the compatibility in Lilium[D].Baoding:Hebei Agricultural University,2009(in Chinese)
[20] Vantuyl J M,Vandien M P. Application of in vitro pollination,ovary culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses[J].Plant Science,1991,74:115-126
[21]李文钿,马丰山.木本植物有性杂交生殖生物学图谱[M].北京:科学出版社,2011Li W D,Ma F S.Map of Reproductive Biology of Woody Plants in Sexual Hybridization[M].Beijing:Science Press,2011(in Chinese)
[22] Shin H,Akira T.Incompatible pollen tube growth and protein composition in styles of Japanese pear following high temperature treatments[J].Euphyical,1989,43:191-196
[23]李玉花,邓明琴,景士西,刘福全.激光和高压静电场对草莓种间杂交不亲和性的影响[J].激光生物学,1995,4(2):636-641Li Y H,Deng M Q,Jiang S X,Liu F Q.The influence of laser and high-voltage electrostatic field on incompatibility of strawberry interspecific hybridization[J].Laster Biology,1995,4(2):636-641(in Chinese)
[24] Gaget M, Villar M, Dumas C. The mentor pollen phenomenon in poplars:A new concept[J].Theortical and Appi lied Genetles,1989,78:129-135