酶法辅助提取海红果中的白藜芦醇
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摘要
以海红果干粉为原料,通过单因素及正交试验,优化海红果中白藜芦醇的酶法辅助醇提工艺,采用比色法进行白藜芦醇的产量检测。结果表明,酶法辅助提取的适宜条件为:p H值4.75,酶解温度50℃,酶解时间120 min,纤维素酶添加量120 mg/g。在该最优条件下,白藜芦醇的产量可达到114.37μg/g,是常规醇提方法的1.72倍。
The dry powder of circassian fruit was used as test material, to study the enzymatic assisted extraction process of resveratrol in it using single-factor and orthogonal experiment optimization, and the content of resverstrol was tested by colorimetric method. The results showed that the suitable extraction conditions for enzyme assisted extraction were p H 4.75, temperature 50 ℃,enzymolysis time 120 min, the additive amount of cellulasewas120 mg/g. In this optimal condition, the yield of resveratrol could reach114.37 μg/g, which was 1.72 times as much as rthanol extraction method only.
The dry powder of circassian fruit was used as test material, to study the enzymatic assisted extraction process of resveratrol in it using single-factor and orthogonal experiment optimization, and the content of resverstrol was tested by colorimetric method. The results showed that the suitable extraction conditions for enzyme assisted extraction were p H 4.75, temperature 50 ℃,enzymolysis time 120 min, the additive amount of cellulasewas120 mg/g. In this optimal condition, the yield of resveratrol could reach114.37 μg/g, which was 1.72 times as much as rthanol extraction method only.
引文
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[18]张初署,禹山林,潘丽娟,等.响应面法优化花生根中白藜芦醇提取工艺研究[J].食品科学,2009,30(6):467-470.
[2]赵福诗,赵丽芹.我国华北地区海红果生产现状及发展前景[J].内蒙古农业科技,2008(2):94.
[3]肖林霞,卢其能,李润根.白藜芦醇的研究进展[J].现代农业科技,2015(24):264.
[4]韩晶晶,刘炜,毕玉平.白藜芦醇的研究进展[J].生物工程学报,2008,39(2):93-97.
[5]李洁,熊兴耀,曾建国,等.白藜芦醇的研究进展[J].中国现代中药,2013,15(2):100-108.
[6]NDIAYE M,JILIPPE C,MUKHTAR H,et al.The grape antioxidant resveratrol for skin disorders:promise,prospects,and challenges[J].Archives of Biochemistry and Biophysis,2011(2):164-170.
[7]TRINCHERI N F,NICOTRA G,FOLLO C.Resveratrol induces cell death in colorectal cancer cells by a novel pathway involving lysosomal cathepsin D[J].Carcinogenesis,2007,28(5):922-931.
[8]MEISHIANG J,LINING C,GEORGE OU,et al.Cancer chemo-preventive activity of resveratrol,anatural product derived from grapes[J].Science,1997(1):218-220.
[9]BRANDAMANTE S,BAREGHI L,VILLA A.Cardiovascular protective effects of resveratrol[J].Cardiovase Drug Rew,2014,22(3):169-188.
[10]丁勇.海红果酒中白藜芦醇及其糖苷的检测方法研究[J].食品研究与开发,2015,36(17):132-135.
[11]钱时权,石亚中.纤维素酶-超声波辅助有机溶剂提取山葡萄渣中白藜芦醇的研究[J].食品工业科技,2012,33(3):210-213.
[12]兰天路,朱宏吉,李少白.纤维素酶-微波提取虎杖中白藜芦醇的工艺[J].化学工业与工程,2008,25(5):395.
[13]李梦青,聂媛,张洁,等.酶解法提取虎杖中白藜芦醇、白藜芦醇苷、大黄素[J].精细化工,2008,25(5):467-470.
[14]汪开拓,马莉,卢霞.超声波辅助提取葡萄皮渣中白藜芦醇工艺的优化研究[J].西南农业学报,2013,29(3):1201-1206.
[15]毛俊霞,张文君,李曦墨.响应面优化葡萄叶中白藜芦醇的提取条件[J].天然产物的研究与开发,2014,26(6):2027-2032.
[16]刘小丽,张伟,符毅文,等.虎杖中白藜芦醇的超声提取及其抗氧化性研究[J].中成药,2011,33(1):150-153.
[17]陈琼玲,薛霖莉,樊迎,等.响应面法优化桑葚果渣中白藜芦醇的提取功率[J].山西农业科学,2016,44(6):836-839.
[18]张初署,禹山林,潘丽娟,等.响应面法优化花生根中白藜芦醇提取工艺研究[J].食品科学,2009,30(6):467-470.