马铃薯种质资源的晚疫病抗性鉴定
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摘要
采用离体叶片接种法,对43份马铃薯种质资源进行晚疫病抗性鉴定、比较和评价。以接种5d后的感病品种‘Désirée’和抗病品种‘加湘1号’叶片症状为对照,鉴定出5份表现为高抗的种质资源,其中包含3个Solanum phurejia和2个S. tuberosum ssp. andigena材料。另外,还鉴定出14份中抗材料(No. 7~20)。结果表明,野生种和安第斯山栽培亚种马铃薯资源的抗性材料较为丰富,可作为晚疫病抗病育种的亲本。
In this study, 43 potato germplasm were evaluated by inoculating Phytophthora infestans on leaves in vitro, and ‘Désirée' and ‘Jiaxiang 1' were used as susceptible and resistance controls,respectively. The results showed that 5 germplasm with high resistance to P. infestans were identified,including 3 Solanum phurejia germplasm(No. 1, No.2, and No. 3) and 2 S. tuberosum ssp. andigena germplasm(No. 4 and No. 5), according to the disease symptoms of ‘Désirée' and ‘Jiaxiang 1' leaves after inoculation for 5 days. In addition, 14 moderate resistant materials(No. 7 to No. 20) were identified. The results indicated that there were plenty of disease resistance germplasm in wild and S.tuberosum ssp. andigena germplasm, which could be used as parent material in late blight resistance breeding.
In this study, 43 potato germplasm were evaluated by inoculating Phytophthora infestans on leaves in vitro, and ‘Désirée' and ‘Jiaxiang 1' were used as susceptible and resistance controls,respectively. The results showed that 5 germplasm with high resistance to P. infestans were identified,including 3 Solanum phurejia germplasm(No. 1, No.2, and No. 3) and 2 S. tuberosum ssp. andigena germplasm(No. 4 and No. 5), according to the disease symptoms of ‘Désirée' and ‘Jiaxiang 1' leaves after inoculation for 5 days. In addition, 14 moderate resistant materials(No. 7 to No. 20) were identified. The results indicated that there were plenty of disease resistance germplasm in wild and S.tuberosum ssp. andigena germplasm, which could be used as parent material in late blight resistance breeding.
引文
[1]罗彦涛,孟润杰,赵建江,韩秀英,马志强,王文桥,张小风.马铃薯晚疫病菌对氟吡菌胺抗性突变体的获得及其生物学性状[J].中国农业科学,2016,49(19):3733-3745.
[2]谢从华.马铃薯产业的现状与发展[J].华中农业大学学报(社会科学版),2012(1):1-4.
[3]Haverkort A J,Boonekamp P M,Rutten R,Jacobsen E,Lotz L A P,Kessel G J T,Visser R G F,van der Vossen E A G.Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification[J].Potato Research,2008,51:47-57.
[4]何烈干,汤洁,熊正葵,马辉刚.马铃薯种质资源对晚疫病的室内及田间抗性鉴定[J].南方农业学报,2018,49(5):898-904.
[5]娄树宝,李庆全,田国奎,徐洪岩,王海燕,王立春,盛万民.马铃薯种质资源晚疫病抗性鉴定与评价[J].黑龙江农业科学,2012(12):11-13.
[6]先丹,郭国雄,冯明议,袁红筑.马铃薯不同品种(系)对马铃薯晚疫病的田间抗性[J].安徽农业科学,2014,42(35):12516-12519.
[7]卢丽丽,曹继芬,潘哲超,刘凌云,赵志坚,杨琼芬.云南省马铃薯主要品种晚疫病抗性评价[J].中国马铃薯,2016,30(6):356-361.
[8]刘甜甜,秦玉芝,林丽婷,熊兴耀,周倩.马铃薯种质资源的晚疫病抗性评价[J].分子植物育种,2018,16(4):1289-1293.
[9]晁祥健,孔维国,张煜,刘生祥,杨煜,李广存.二倍体马铃薯资源现状及应用研究进展[J].山东农业科学,2008,7:49-53.
[10]Tiwari J K,Siddappa S,Singh B P,Kaushik S K,Chakrabarti S K,Bhardwaj V,Chandel P.Molecular markers for late blight resistance breeding of potato:an update[J].Plant Breeding,2013,132(3):237-245.
[11]Li G,Huang S,Guo X,Li Y,Yang Y,Guo Z,Kuang H,Rietman H,Bergervoet M,Vleeshouwers V G,van der Vossen EA,Qu D,Visser R G,Jacobsen E,Vossen J H.Cloning and characterization of R3b;members of the R3 superfamily of late blight resistance genes show sequence and functional divergence[J].Molecular Plant-Microbe Interactions,2011,24(10):1132-1142.
[12]Rodewald J,Trognitz B.Solanum resistance genes against Phytophthora infestans and their corresponding avirulence genes[J].Molecular Plant Pathology,2013,14(7):740-757.
[13]Vossen J H,van Arkel G,Bergervoet M,Jo K R,Jacobsen E,Visser R G.The Solanum demissum R8 late blight resistance gene is an Sw-5 homologue that has been deployed worldwide in late blight resistant varieties[J].Theoretical and Applied Genetics,2016,129(9):1785-1796.
[14]Du Y,Berg J,Govers F,Bouwmeester K.Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1[J].New Phytologist,2015,207(3):735-747.
[15]Song J,Bradeen J M,Naess S K,Raasch J A,Wielgus S M,Haberlach G T,Liu J,Kuang H,Austin-Phillips S,Buell C R,Helgeson J P,Jiang J.Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(16):9128-9133.
[16]Lokossou A A,Park T H,van Arkel G,Arens M,Ruyte-Spira C,Morales J,Whisson S C,Birch P R,Visser R G,Jacobsen E,van der Vossen E A.Exploiting knowledge of R/Avr genes to rapidly clone a new LZ-NBS-LRR family of late blight resistance genes from potato linkage group IV[J].Molecular Plant-Microbe Interactions,2009,22(6):630-641.
[17]Oh S K,Young C,Lee M,Oliva R,Bozkurt T O,Cano L M,Win J,Bos J I,Liu H Y,van Damme M,Morgan W,Choi D,Van der Vossen E A,Vleeshouwers V G,Kamoun S.In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes,including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2[J].Plant Cell,2009,21(9):2928-2947.
[18]Pel M A,Foster S J,Park T H,Rietman H,van Arkel G,Jones J D,Van Eck H J,Jacobsen E,Visser R G,Van der Vossen E A.Mapping and cloning of late blight resistance genes from Solanum venturii using an interspecific candidate gene approach[J].Molecular Plant-Microbe Interactions,2009,22(5):601-615.
[19]Cooke D E,Cano L M,Raffaele S,Bain R A,Cooke L R,Etherington G J,Deahl K L,Farrer R A,Gilroy E M,Goss E M,Grünwald N J,Hein I,Mac Lean D,Mc Nicol J W,Randall E,Oliva R F,Pel M A,Shaw D S,Squires J N,Taylor M C,Vleeshouwers V G,Birch P R,Lees A K,Kamoun S.Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen[J].PLoS Pathogens,2012,8(10):e1002940.
[20]Raffaele S,Win J,Cano L M,Kamoun S.Analyses of genome architecture and gene expression reveal novel candidate virulence factors in the secretome of Phytophthora infestans[J].BMC Genomics,2010,11:637.
[21]王洪洋,田振东.基于晚疫病菌效应子识别策略挖掘马铃薯潜在抗病基因资源[J].园艺学报,2018,45(7):1305-1313.
[22]Vleeshouwers V G A A,Dooijeweert W V,Keizer L C P,Sijpkes L,Govers F,Colon L T.A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation[J].European Journal of Plant Pathology,1999,105(3):241-250.
[23]鲁绍凤,杨艳丽,胡先奇,罗文富,李丽娟.云南省马铃薯主栽品种对晚疫病的抗性分析[J].云南农业大学学报,2006,21(5):586-590.
[24]娜仁,张笑宇,张之为,杜衍平,赵君.马铃薯不同品种(系)对晚疫病抗性鉴定[J].作物杂志,2010(4):59-61.
[25]杨丽娜,段国华,覃雁瑜,祝雯,潘哲超,隋启君,詹家馁.2012年云南省会泽县马铃薯晚疫病菌小种结构分析[J].热带作物学报,2016,37(1):158-163.
[26]Kim H J,Lee H R,Jo K R,Mortazavian S M,Huigen D J,Evenhuis B,Kessel G,Visser R G,Jacobsen E,Vossen J H.Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked Rgenes[J].Theoretical and Applied Genetics,2012,124(5):923-935.
[2]谢从华.马铃薯产业的现状与发展[J].华中农业大学学报(社会科学版),2012(1):1-4.
[3]Haverkort A J,Boonekamp P M,Rutten R,Jacobsen E,Lotz L A P,Kessel G J T,Visser R G F,van der Vossen E A G.Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification[J].Potato Research,2008,51:47-57.
[4]何烈干,汤洁,熊正葵,马辉刚.马铃薯种质资源对晚疫病的室内及田间抗性鉴定[J].南方农业学报,2018,49(5):898-904.
[5]娄树宝,李庆全,田国奎,徐洪岩,王海燕,王立春,盛万民.马铃薯种质资源晚疫病抗性鉴定与评价[J].黑龙江农业科学,2012(12):11-13.
[6]先丹,郭国雄,冯明议,袁红筑.马铃薯不同品种(系)对马铃薯晚疫病的田间抗性[J].安徽农业科学,2014,42(35):12516-12519.
[7]卢丽丽,曹继芬,潘哲超,刘凌云,赵志坚,杨琼芬.云南省马铃薯主要品种晚疫病抗性评价[J].中国马铃薯,2016,30(6):356-361.
[8]刘甜甜,秦玉芝,林丽婷,熊兴耀,周倩.马铃薯种质资源的晚疫病抗性评价[J].分子植物育种,2018,16(4):1289-1293.
[9]晁祥健,孔维国,张煜,刘生祥,杨煜,李广存.二倍体马铃薯资源现状及应用研究进展[J].山东农业科学,2008,7:49-53.
[10]Tiwari J K,Siddappa S,Singh B P,Kaushik S K,Chakrabarti S K,Bhardwaj V,Chandel P.Molecular markers for late blight resistance breeding of potato:an update[J].Plant Breeding,2013,132(3):237-245.
[11]Li G,Huang S,Guo X,Li Y,Yang Y,Guo Z,Kuang H,Rietman H,Bergervoet M,Vleeshouwers V G,van der Vossen EA,Qu D,Visser R G,Jacobsen E,Vossen J H.Cloning and characterization of R3b;members of the R3 superfamily of late blight resistance genes show sequence and functional divergence[J].Molecular Plant-Microbe Interactions,2011,24(10):1132-1142.
[12]Rodewald J,Trognitz B.Solanum resistance genes against Phytophthora infestans and their corresponding avirulence genes[J].Molecular Plant Pathology,2013,14(7):740-757.
[13]Vossen J H,van Arkel G,Bergervoet M,Jo K R,Jacobsen E,Visser R G.The Solanum demissum R8 late blight resistance gene is an Sw-5 homologue that has been deployed worldwide in late blight resistant varieties[J].Theoretical and Applied Genetics,2016,129(9):1785-1796.
[14]Du Y,Berg J,Govers F,Bouwmeester K.Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1[J].New Phytologist,2015,207(3):735-747.
[15]Song J,Bradeen J M,Naess S K,Raasch J A,Wielgus S M,Haberlach G T,Liu J,Kuang H,Austin-Phillips S,Buell C R,Helgeson J P,Jiang J.Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(16):9128-9133.
[16]Lokossou A A,Park T H,van Arkel G,Arens M,Ruyte-Spira C,Morales J,Whisson S C,Birch P R,Visser R G,Jacobsen E,van der Vossen E A.Exploiting knowledge of R/Avr genes to rapidly clone a new LZ-NBS-LRR family of late blight resistance genes from potato linkage group IV[J].Molecular Plant-Microbe Interactions,2009,22(6):630-641.
[17]Oh S K,Young C,Lee M,Oliva R,Bozkurt T O,Cano L M,Win J,Bos J I,Liu H Y,van Damme M,Morgan W,Choi D,Van der Vossen E A,Vleeshouwers V G,Kamoun S.In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes,including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2[J].Plant Cell,2009,21(9):2928-2947.
[18]Pel M A,Foster S J,Park T H,Rietman H,van Arkel G,Jones J D,Van Eck H J,Jacobsen E,Visser R G,Van der Vossen E A.Mapping and cloning of late blight resistance genes from Solanum venturii using an interspecific candidate gene approach[J].Molecular Plant-Microbe Interactions,2009,22(5):601-615.
[19]Cooke D E,Cano L M,Raffaele S,Bain R A,Cooke L R,Etherington G J,Deahl K L,Farrer R A,Gilroy E M,Goss E M,Grünwald N J,Hein I,Mac Lean D,Mc Nicol J W,Randall E,Oliva R F,Pel M A,Shaw D S,Squires J N,Taylor M C,Vleeshouwers V G,Birch P R,Lees A K,Kamoun S.Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen[J].PLoS Pathogens,2012,8(10):e1002940.
[20]Raffaele S,Win J,Cano L M,Kamoun S.Analyses of genome architecture and gene expression reveal novel candidate virulence factors in the secretome of Phytophthora infestans[J].BMC Genomics,2010,11:637.
[21]王洪洋,田振东.基于晚疫病菌效应子识别策略挖掘马铃薯潜在抗病基因资源[J].园艺学报,2018,45(7):1305-1313.
[22]Vleeshouwers V G A A,Dooijeweert W V,Keizer L C P,Sijpkes L,Govers F,Colon L T.A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation[J].European Journal of Plant Pathology,1999,105(3):241-250.
[23]鲁绍凤,杨艳丽,胡先奇,罗文富,李丽娟.云南省马铃薯主栽品种对晚疫病的抗性分析[J].云南农业大学学报,2006,21(5):586-590.
[24]娜仁,张笑宇,张之为,杜衍平,赵君.马铃薯不同品种(系)对晚疫病抗性鉴定[J].作物杂志,2010(4):59-61.
[25]杨丽娜,段国华,覃雁瑜,祝雯,潘哲超,隋启君,詹家馁.2012年云南省会泽县马铃薯晚疫病菌小种结构分析[J].热带作物学报,2016,37(1):158-163.
[26]Kim H J,Lee H R,Jo K R,Mortazavian S M,Huigen D J,Evenhuis B,Kessel G,Visser R G,Jacobsen E,Vossen J H.Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked Rgenes[J].Theoretical and Applied Genetics,2012,124(5):923-935.