赛甫拉合剂对对乙酰氨基酚致大鼠肝损伤的保护作用
|
摘要
目的研究赛甫拉合剂对对乙酰氨基酚致大鼠肝损伤的保护作用。方法将SD大鼠随机分为对照组和实验组,实验组按公斤体质量灌胃对乙酰氨基酚750 mg,30 d后,将实验组随机分为对乙酰氨基酚肝损伤模型组(APAP模型组)、水飞蓟宾(按公斤体质量21 mg)组、赛甫拉合剂(以药材粉末计算)按公斤体质量低(150 mg)、中(300 mg)和高(600 mg)剂量组,每组12只。各组于每日上午给药,同时于每日下午(除对照组外)按公斤体质量给予对乙酰氨基酚400 mg,连续4周,于4周后取血液和肝脏测定相关指标。结果与对照组比较,APAP模型组血清ALT、AST、ALP、TNF-α、MDA和ROS水平均明显增高(P<0.01),GSH和SOD水平明显降低(P<0.01)。与APAP模型组比较,赛甫拉合剂各剂量组与水飞蓟宾组血清ALT、AST、ALP、TNF-α、MDA和ROS水平均降低(P<0.05);GSH和SOD水平升高(P<0.05)。结论赛甫拉合剂对对乙酰氨基酚造成的大鼠肝损伤有保护作用。
Objective To investigate the protective effects of SFL Mixture on rats with acetaminophen(APAP)-induced liver injury.Methods Rats were randomly divided into control group and experimental group,and the experimental group were according to body weight administrated with acetaminophen for 750 mg.After 30 d,the experimental group were randomly divided into APAP-induced liver injury group(APAP model),silibinin group(21 mg per kiogram) as well as SFL Mixture low dose(150 mg per kiogram),middle dose(300 mg per kiogram),and high dose(600 mg per kiogram),12 rats in each group.The SFL Mixture and silibinin group were given drug treatment in the morning.Meanwhile,the other groups(except the control group) continued to receive 400 mg acetaminophen in the afternoon for 4 weeks.4 weeks later,their blood and organs were collected and the related indicators of hepatic injury were measured.Results Compared with the control group,the serum ALT,AST,ALP,TNF-α,MDA and ROS levels of the APAP-induced liver injury group were increased significantly(P<0.01).And the serum GSH and SOD levels decreased significantly(P<0.01).Compared with the APAP model group,significantly decreased in the AST,ALT,ALP,TNF-α,MDA and ROS levels of SFL Misture and silibinin groups(P<0.05).And GSH and SOD levels increased significantly(P<0.05).Conclusion SFL Mixture can protect APAP-induced liver injury in rats.
Objective To investigate the protective effects of SFL Mixture on rats with acetaminophen(APAP)-induced liver injury.Methods Rats were randomly divided into control group and experimental group,and the experimental group were according to body weight administrated with acetaminophen for 750 mg.After 30 d,the experimental group were randomly divided into APAP-induced liver injury group(APAP model),silibinin group(21 mg per kiogram) as well as SFL Mixture low dose(150 mg per kiogram),middle dose(300 mg per kiogram),and high dose(600 mg per kiogram),12 rats in each group.The SFL Mixture and silibinin group were given drug treatment in the morning.Meanwhile,the other groups(except the control group) continued to receive 400 mg acetaminophen in the afternoon for 4 weeks.4 weeks later,their blood and organs were collected and the related indicators of hepatic injury were measured.Results Compared with the control group,the serum ALT,AST,ALP,TNF-α,MDA and ROS levels of the APAP-induced liver injury group were increased significantly(P<0.01).And the serum GSH and SOD levels decreased significantly(P<0.01).Compared with the APAP model group,significantly decreased in the AST,ALT,ALP,TNF-α,MDA and ROS levels of SFL Misture and silibinin groups(P<0.05).And GSH and SOD levels increased significantly(P<0.05).Conclusion SFL Mixture can protect APAP-induced liver injury in rats.
引文
[1] 杨伟俊,孙殿甲.对乙酰氨基酚研究进展[J].西北药学杂志,2002,17(6):283-285.
[2] Lin Z,Wu F,Lin S,et al.Adiponectin protects against acetaminophen-induced mitochondrial dysfunction and acute liver injury by promoting autophagy in mice[J].J Hepatol,2014,61(4):825-831.
[3] Baek H J,Lee Y M,Kim T H,et al.Caspase-3/7-mediated cleavage of β2-spectrin is required for acetaminophen-induced liver damage[J].Int J Biol Sci,2016,12(2):172-183.
[4] Uchida N S,Silva-Filho S E,Aguiar R P,et al.Protective effect of Cymbopogon citratus essential oil in experimental model of acet-aminophen-induced liver injury[J].Am J Chin Med,2017,45(3):515-532.
[5] 任东东,邓存良.药物性肝损伤的研究进展[J].西南军医,2014,16(6):670-672.
[6] 杨红洁,王霖.药物性肝损伤临床分析[J].中国医药指南,2012,10(9):203-204.
[7] 庄敏之,王亚平,郑昕,等.护肝解毒方对药物性肝损伤及相关炎症因子作用研究[J].中国中西医结合消化杂志,2016,24(1):26-28.
[8] 朱依谆,殷明,邹莉波,等.药理学[M].北京:人民卫生出版社,2014:316-317.
[9] Jaeschke H,McGill M R,Williams C D,et al.Current issues with acetaminophen hepatotoxicity-a clinically relevant model to test the efficacy of natural products[J].Life Sci,2011,88(17/18):737-745.
[10] Jaeschke H,McGill M R,Ramachandran A.Oxidant stress,mitochondria,and cell death mechanisms in drug-induced liver injury:lessons learned from acetaminophen hepatotoxicity[J].Drug Metab Rev,2012,44(1):88-106.
[11] Mason R P,Fischer V.Free radicals of acetaminophen:their subsequent reactions and toxicological significance[J].Fed Proc,1986,45(10):2493-2499.
[12] Knockaert L,Descatoire V,Vadrot N,et al.Mitochondrial CYP2E1 is sufficient to mediate oxidative stress and cytotoxicity induced by ethanol and acetaminophen[J].Toxiol In Vitro,2011,25(2):475-484.
[13] 刘燕,姚华,黄华.维吾尔药保肝作用的研究进展[J].西北药学杂志,2018,33(2):278-283.
[14] 孙园,李乐.天山堇菜化学成分及生物活性研究[J].海峡药学,2016,28(3):51-53.
[15] 刘勇民.维吾尔药志:上册[M].乌鲁木齐:新疆科技卫生出版社,1999:35-37.
[16] 国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:200.
[17] 姜君君,郭玉婷,施洋,等.维药小枝玫瑰花中没食子酸含量测定[J].新疆医科大学学报,2016,39(12):1563-1565.
[18] 米克热木·沙衣布扎提,蒋志惠,吾买尔江·牙合甫,等.传统维药骆驼刺刺糖有效成分及药理作用研究进展[J].中药材,2016,39(10):2397-2399.
[19] 吾买尔江·牙合甫.维药刺糖对小鼠急性肾损伤的保护作用初探[A]//姚刚.全国动物生理化第七届全国代表大会暨第十三次学术交流会论文集[C].北京:中国畜牧兽医学会,2014:280-281.
[20] Chen W T,Yang C L,Yin M C.Protective effects from Houttuynia cordata aqueous extract against acetaminophen-induced liver injury[J].Bio Med,2014,4(1):24-28.
[2] Lin Z,Wu F,Lin S,et al.Adiponectin protects against acetaminophen-induced mitochondrial dysfunction and acute liver injury by promoting autophagy in mice[J].J Hepatol,2014,61(4):825-831.
[3] Baek H J,Lee Y M,Kim T H,et al.Caspase-3/7-mediated cleavage of β2-spectrin is required for acetaminophen-induced liver damage[J].Int J Biol Sci,2016,12(2):172-183.
[4] Uchida N S,Silva-Filho S E,Aguiar R P,et al.Protective effect of Cymbopogon citratus essential oil in experimental model of acet-aminophen-induced liver injury[J].Am J Chin Med,2017,45(3):515-532.
[5] 任东东,邓存良.药物性肝损伤的研究进展[J].西南军医,2014,16(6):670-672.
[6] 杨红洁,王霖.药物性肝损伤临床分析[J].中国医药指南,2012,10(9):203-204.
[7] 庄敏之,王亚平,郑昕,等.护肝解毒方对药物性肝损伤及相关炎症因子作用研究[J].中国中西医结合消化杂志,2016,24(1):26-28.
[8] 朱依谆,殷明,邹莉波,等.药理学[M].北京:人民卫生出版社,2014:316-317.
[9] Jaeschke H,McGill M R,Williams C D,et al.Current issues with acetaminophen hepatotoxicity-a clinically relevant model to test the efficacy of natural products[J].Life Sci,2011,88(17/18):737-745.
[10] Jaeschke H,McGill M R,Ramachandran A.Oxidant stress,mitochondria,and cell death mechanisms in drug-induced liver injury:lessons learned from acetaminophen hepatotoxicity[J].Drug Metab Rev,2012,44(1):88-106.
[11] Mason R P,Fischer V.Free radicals of acetaminophen:their subsequent reactions and toxicological significance[J].Fed Proc,1986,45(10):2493-2499.
[12] Knockaert L,Descatoire V,Vadrot N,et al.Mitochondrial CYP2E1 is sufficient to mediate oxidative stress and cytotoxicity induced by ethanol and acetaminophen[J].Toxiol In Vitro,2011,25(2):475-484.
[13] 刘燕,姚华,黄华.维吾尔药保肝作用的研究进展[J].西北药学杂志,2018,33(2):278-283.
[14] 孙园,李乐.天山堇菜化学成分及生物活性研究[J].海峡药学,2016,28(3):51-53.
[15] 刘勇民.维吾尔药志:上册[M].乌鲁木齐:新疆科技卫生出版社,1999:35-37.
[16] 国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:200.
[17] 姜君君,郭玉婷,施洋,等.维药小枝玫瑰花中没食子酸含量测定[J].新疆医科大学学报,2016,39(12):1563-1565.
[18] 米克热木·沙衣布扎提,蒋志惠,吾买尔江·牙合甫,等.传统维药骆驼刺刺糖有效成分及药理作用研究进展[J].中药材,2016,39(10):2397-2399.
[19] 吾买尔江·牙合甫.维药刺糖对小鼠急性肾损伤的保护作用初探[A]//姚刚.全国动物生理化第七届全国代表大会暨第十三次学术交流会论文集[C].北京:中国畜牧兽医学会,2014:280-281.
[20] Chen W T,Yang C L,Yin M C.Protective effects from Houttuynia cordata aqueous extract against acetaminophen-induced liver injury[J].Bio Med,2014,4(1):24-28.