钙对母鼠氟暴露致仔鼠自主性活动和学习记忆的影响
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摘要
目的探讨饲料钙对母鼠氟暴露致仔鼠自主性活动和学习记忆的影响。方法选用健康初断乳SD雌性大鼠75只,雄性25只,雌鼠随机分为对照组、高氟组、低钙组、低钙高氟组和高钙高氟组,饲养3个月后,雌雄鼠合笼交配产仔鼠,取14及28日龄仔鼠进行开场行为和Morris水迷宫实验。结果开场行为观测结果表明,与对比组相比,各日龄高氟组与高氟低钙组焦虑感增强,兴奋性或自发性活动能力下降,差异有统计学意义(P<0.05或P<0.01);水迷宫学习记忆观测结果显示,与对照组相比,各日龄高氟组与高氟低钙组学习记忆下降,差异有统计学意义(P<0.05或P<0.01);低钙组与高氟高钙组学习记忆改善有所改善。结论高钙饲料能明显改善母鼠氟暴露致仔鼠学习记忆的损伤,母鼠氟暴露致子代学习记忆损伤有性别差异,雌性仔鼠的损伤大于雄性仔鼠。
Objective To disscuss the effects of feed calcium on the autonomic activity and learning and memory in neonatal offspring rats induced by fluoride exposure of mother rats before pregnancy. Methods Seventy-five newly weaned healthy Sprague-Dawley female rats and twenty-five male rats, female rats were randomly divided into control group, high fluoride group, low calcium group, low calcium with high fluoride group and high calcium with high fluoride group. After 3-month fluroride exposure by drinking water, the female rats were mated to male rats and delivered naturally. And the 14 and 28-day-old offspring rats were examined by the opening behavior and the Morris water maze test. Result The observations of the opening behavior showed that,compared with the control group,the anxiety of the high fluoride group and the high fluoride with low calcium group increased(P<0.05, P<0.01), and the excitability or spontaneous activity decreased(P<0.05, P<0.01). Water maze learning and memory observations showed that,compared with the control group,learning and memory significantly decreased in high fluoride group and high fluoride with low calcium group(P<0.05, P<0.01). In low calcium group and high fluoride with high calcium group, learning memory improvement has been improved. Conclusion High calcium diet can significantly improve the learning and memory impairment of neonatal offspring rats induced by fluoride exposure in mother rats before pregnancy. There is gender difference in the learning and memory impairment of the offspring caused by fluoride exposure in mother rats before pregnancy. The impairment of female rats is greater than that of male rats.
Objective To disscuss the effects of feed calcium on the autonomic activity and learning and memory in neonatal offspring rats induced by fluoride exposure of mother rats before pregnancy. Methods Seventy-five newly weaned healthy Sprague-Dawley female rats and twenty-five male rats, female rats were randomly divided into control group, high fluoride group, low calcium group, low calcium with high fluoride group and high calcium with high fluoride group. After 3-month fluroride exposure by drinking water, the female rats were mated to male rats and delivered naturally. And the 14 and 28-day-old offspring rats were examined by the opening behavior and the Morris water maze test. Result The observations of the opening behavior showed that,compared with the control group,the anxiety of the high fluoride group and the high fluoride with low calcium group increased(P<0.05, P<0.01), and the excitability or spontaneous activity decreased(P<0.05, P<0.01). Water maze learning and memory observations showed that,compared with the control group,learning and memory significantly decreased in high fluoride group and high fluoride with low calcium group(P<0.05, P<0.01). In low calcium group and high fluoride with high calcium group, learning memory improvement has been improved. Conclusion High calcium diet can significantly improve the learning and memory impairment of neonatal offspring rats induced by fluoride exposure in mother rats before pregnancy. There is gender difference in the learning and memory impairment of the offspring caused by fluoride exposure in mother rats before pregnancy. The impairment of female rats is greater than that of male rats.
引文
[1] Choubisa SL. Status of fluorosis in animals[J]. Proc Natl Acad Sci India Sect B Biol Sci, 2012, 82(3): 331-339.
[2] 吴益,赵倩,章子贵,等. 慢性氟中毒致大鼠肾脏损伤用[J]. 中国公共卫生,2010, 26(9): 66-67.
[3] 孙天水, 席淑华. 高氟对中枢神经系统损伤机制的研究进展[J]. 环境与健康杂志, 2016, 33(4): 368-371.
[4] 张璟, 祝文静, 章仔贵. 孕鼠氟暴露对仔鼠学习记忆能力的影响[J]. 中国公共卫生, 2009, 25(11): 1347-1348.
[5] Basha PM, Rai P, Begum S. Evaluation of fluoride-induced oxidative stress in rat brain: a multigeneration study[J]. Biol Trace Elem Res, 2011, 142(3): 623-637.
[6] 谢颖, 钟才高. 对化学毒物诱导肝细胞线粒体损伤的保护作用机制研究进展[J]. 中国药理学与毒理学杂志, 2012, 26(5): 684-687.
[7] Shanthakumari D, Srinivasalu S, Subramanian S. Effeet of fluoride intoxicationon lipidperoxidationand antioxidatnt status in experimental rats[J]. Toxicology, 2004, 204(2-3):219-228.
[8] Fujita T, Palmieri GMA. Calcium paradox disease:Calcium deficiency prompting secondary hyperparathyroidism and cellular calcium overload[J]. J Bone Miner Metab, 2000, 18(3): 109-125.
[9] 万桂敏, 陈志, 莫志亚, 等. 地方性氟中毒患者多项检验指标的测定及分析[J]. 中国地方病学杂志, 2001, 20(2): 137-139.
[10] 张文岚, 孙玲, 薛立娟, 等. 营养性低钙与慢性氟中毒大鼠细胞内钙超载相关性研究[J]. 中国地方病学杂志, 2006, 25(6): 622-624.
[11] 韩爱军, 刘盘龙, 梅予锋. 慢性氟中毒对大鼠硬组织氟含量的影响[J]. 中国医药导报, 2013, 10(3): 29-30, 34.
[12] Lou LD, Guan ZZ, Liu YJ, et al. The inuence of chronic uorosis on mitochondrial dynamics morphology and distribution in cortical neurons of the rat brain[J]. Arch Toxicol, 2013, 87(3): 449-457.
[13] 葛亚明, 宁红梅, 刘俊伟, 等. 氟对不同世代大鼠大脑皮质细胞凋亡的影响[J]. 中国畜牧兽医, 2010, 37(7): 44-46.
[14]Gao Q,Liu YJ,Guan ZZ.Decreased learning and memory ability in rats with flurosis:Increases oxidative stress and reduced cholinesterase activity in the brain[J].Fluoride,2009,42:277-285.
[15]Lea RC,Inaer MR,Cludiao DC,et al.Subchronic flueoride intake induces impairment in habituation and active avoidance task in rats[J].Eur J Pharmacol,2008,579(1/3):196-201.
[16]章子贵,徐晓虹,申秀英,等.慢性氟中毒对小鼠学习记忆行为和运动耐力的影响[J].中国公共卫生,1999,15(3):186-187.
[17]Zheng Z,Sun Y,Ke L,et al.Molecular mechanism of brain impairment caused by drinking-acquired fluorosis and selenium intervention[J].Environ Toxicol Pharmacol,2016,43:134-139.
[18]霍梅俊,王金明,姚冰芬,等.不同钙水平对氟中毒大鼠肾脏功能损伤的影响[J].畜牧兽医学报,2012,43(9):1483-1490.
[19]徐辉,赵志涛,井玲,等.氟中毒大鼠骨组织中内质网应激实验研究[J].中国地方病学杂志,2009,28(1):36-40.
[2] 吴益,赵倩,章子贵,等. 慢性氟中毒致大鼠肾脏损伤用[J]. 中国公共卫生,2010, 26(9): 66-67.
[3] 孙天水, 席淑华. 高氟对中枢神经系统损伤机制的研究进展[J]. 环境与健康杂志, 2016, 33(4): 368-371.
[4] 张璟, 祝文静, 章仔贵. 孕鼠氟暴露对仔鼠学习记忆能力的影响[J]. 中国公共卫生, 2009, 25(11): 1347-1348.
[5] Basha PM, Rai P, Begum S. Evaluation of fluoride-induced oxidative stress in rat brain: a multigeneration study[J]. Biol Trace Elem Res, 2011, 142(3): 623-637.
[6] 谢颖, 钟才高. 对化学毒物诱导肝细胞线粒体损伤的保护作用机制研究进展[J]. 中国药理学与毒理学杂志, 2012, 26(5): 684-687.
[7] Shanthakumari D, Srinivasalu S, Subramanian S. Effeet of fluoride intoxicationon lipidperoxidationand antioxidatnt status in experimental rats[J]. Toxicology, 2004, 204(2-3):219-228.
[8] Fujita T, Palmieri GMA. Calcium paradox disease:Calcium deficiency prompting secondary hyperparathyroidism and cellular calcium overload[J]. J Bone Miner Metab, 2000, 18(3): 109-125.
[9] 万桂敏, 陈志, 莫志亚, 等. 地方性氟中毒患者多项检验指标的测定及分析[J]. 中国地方病学杂志, 2001, 20(2): 137-139.
[10] 张文岚, 孙玲, 薛立娟, 等. 营养性低钙与慢性氟中毒大鼠细胞内钙超载相关性研究[J]. 中国地方病学杂志, 2006, 25(6): 622-624.
[11] 韩爱军, 刘盘龙, 梅予锋. 慢性氟中毒对大鼠硬组织氟含量的影响[J]. 中国医药导报, 2013, 10(3): 29-30, 34.
[12] Lou LD, Guan ZZ, Liu YJ, et al. The inuence of chronic uorosis on mitochondrial dynamics morphology and distribution in cortical neurons of the rat brain[J]. Arch Toxicol, 2013, 87(3): 449-457.
[13] 葛亚明, 宁红梅, 刘俊伟, 等. 氟对不同世代大鼠大脑皮质细胞凋亡的影响[J]. 中国畜牧兽医, 2010, 37(7): 44-46.
[14]Gao Q,Liu YJ,Guan ZZ.Decreased learning and memory ability in rats with flurosis:Increases oxidative stress and reduced cholinesterase activity in the brain[J].Fluoride,2009,42:277-285.
[15]Lea RC,Inaer MR,Cludiao DC,et al.Subchronic flueoride intake induces impairment in habituation and active avoidance task in rats[J].Eur J Pharmacol,2008,579(1/3):196-201.
[16]章子贵,徐晓虹,申秀英,等.慢性氟中毒对小鼠学习记忆行为和运动耐力的影响[J].中国公共卫生,1999,15(3):186-187.
[17]Zheng Z,Sun Y,Ke L,et al.Molecular mechanism of brain impairment caused by drinking-acquired fluorosis and selenium intervention[J].Environ Toxicol Pharmacol,2016,43:134-139.
[18]霍梅俊,王金明,姚冰芬,等.不同钙水平对氟中毒大鼠肾脏功能损伤的影响[J].畜牧兽医学报,2012,43(9):1483-1490.
[19]徐辉,赵志涛,井玲,等.氟中毒大鼠骨组织中内质网应激实验研究[J].中国地方病学杂志,2009,28(1):36-40.