2种应激诱导大鼠迷走复合体神经元的Fos表达
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摘要
将大鼠随机分为束缚浸水应激组、束缚应激组和对照组,经1 h应激处理后,采用免疫组化法标记迷走复合体中神经元的Fos基因表达,在亚区和亚核水平上进行组间比较,研究2种应激对大鼠迷走复合体神经元Fos表达的影响。结果表明:束缚浸水应激组迷走背核的吻、中和尾3段Fos阳性神经数量均显著增加(P<0.01或<0.05),而束缚应激组仅尾段Fos表达显著增加(P<0.01);在孤束核的吻、中和尾3段,2种应激引起不同孤束亚核的神经元Fos表达的增加(P<0.01);不同应激处理后迷走复合体的不同亚区或亚核神经元的活动会增强,可能与不同应激性胃肠机能障碍的表现密切相关。
The effects of restraint water-immersion stress( RWIS) and restraint stress( RS) on neuronal activities of the dorsal vagal complex( DVC) including the nucleus tractus solitarious( NTS) and the dorsal motor nucleus of the vagus( DMV) were compared.An immunohistochemical detection of Fos protein was utilized to assess neuronal activation. The results show that RWIS induces the increase of Fos expressions in all the rostral,intermediate and caudal DMV in the RWIS group( P < 0. 01 or 0. 05) while only in the caudal DMV in the RS group( P < 0. 01). In addition,RWIS and RS induce the increase of neuronal Fos expressions in different sulnucleus of NTS. Differences of neuronal Fos expressions induced by these two stress are apparent in the medial,ventrolateral,dorsolateral and commissural NTS( P < 0. 01). These results indicate that activated neurons separately induce by RWIS and RS distribute in different subdivisions of the DVC,which may be responsible for the different stress-induced effects on gastric function.
The effects of restraint water-immersion stress( RWIS) and restraint stress( RS) on neuronal activities of the dorsal vagal complex( DVC) including the nucleus tractus solitarious( NTS) and the dorsal motor nucleus of the vagus( DMV) were compared.An immunohistochemical detection of Fos protein was utilized to assess neuronal activation. The results show that RWIS induces the increase of Fos expressions in all the rostral,intermediate and caudal DMV in the RWIS group( P < 0. 01 or 0. 05) while only in the caudal DMV in the RS group( P < 0. 01). In addition,RWIS and RS induce the increase of neuronal Fos expressions in different sulnucleus of NTS. Differences of neuronal Fos expressions induced by these two stress are apparent in the medial,ventrolateral,dorsolateral and commissural NTS( P < 0. 01). These results indicate that activated neurons separately induce by RWIS and RS distribute in different subdivisions of the DVC,which may be responsible for the different stress-induced effects on gastric function.
引文
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[3]BORNSTEIN J C,COSTA M,GRIDER J R.Enteric motor and interneuronal circuits controlling motility[J].Neurogastroenterol Motil,2004,16(SupplⅠ):34-38.
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[14]韩济生.神经科学原理:上[M].2版.北京:北京医科大学出版社,1999:410-417.
[15]ZHANG Y Y,CAO G H,ZHU W X,et al.Comparative study of cFos expression in rat dorsal vagal complex and nucleus ambiguus induced by different durations of restraint water-immersion stress[J].Chin J Physiol,2009,52(3):143-150.
[16]TAKAHASHI C,OHATA H,SHIBASAKI T.Corticotropin-releasing factor(CRF)receptor subtypes in mediating neuronal activation of brain areas involved in responses to intracerebroventricular CRF and stress in rats[J].Peptides,2011,32(12):2384-2393.
[17]BONAZ B,TACHE Y.Induction of Fos immunoreactivity in the rat brain after cold-restraint induced gastric lesions and fecal excretion[J].Brain Res,1994,652(1):56-64.
[18]宿长军,段丽,饶志仁.大鼠束缚后脑内Fos蛋白的表达[J].解剖学报,2005,36(2):123-126.
[19]PACK K,PALKOVITS M.Stressor specificity of central neuroendocrine responses:implications for stress-related disorders[J].Endocrine Reviews,2001,22(4):502-548.
[20]CRUZ M T,MURPHY E C,SAHIBZADA N,et al.A reevaluation of the effects of stimulation of the dorsal motor nucleus of the vagus on gastric motility in the rat[J].Am J Physiol Regul Integr Comp Physiol,2007,292(1):R291-R307.
[21]ZHOU S Y,LU Y X,YAO H R,et al.Spatial organization of neurons in the dorsal motor nucleus of the vagus synapsing with intragastric cholinergic and nitric oxide/VIP neurons in the rat[J].Am J Physiol Gastrointest Liver Physiol,2008,294(5):G1201-G1209.
[22]朱长庚.神经解剖学[M].2版.北京:人民卫生出版社,2009:454-459.
[23]PAXINOS G,WATSON C.The rat brain in stereotaxic coordinates[M].5th ed.Burlington:Elsevier Academic Press,2005.
[24]ZHAO D Q,AI H B.Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain[J].Plos One,2011,6(8):e233622.
[2]COSTA M,BROOKES S J H,HENNIG G W.Anatomy and physiology of the enteric nervous system[J].Gut,2000,47(SupplⅣ):15-19.
[3]BORNSTEIN J C,COSTA M,GRIDER J R.Enteric motor and interneuronal circuits controlling motility[J].Neurogastroenterol Motil,2004,16(SupplⅠ):34-38.
[4]CHANG H Y,MASHIMO H,GOYAL R K.Musings on the wanderer:what’s new in our understanding of vago-vagal reflex?Ⅳ.current concepts of vagal efferent projections to the gut[J].Ajp Gastrointest&Liver Physiology,2003,284(3):G357-G366.
[5]TAKEDA H,OHNISHI S,MUTO S,et al.Mo1782 association of abnormal ghrelin dynamics with delayed gastric emptying in acute restraint-stressed mice[J].Gastroenterology,2014,146(5 Suppl1):S-658.
[6]NAKADE Y,TSUCHIDA D,FUKUDA H,et al.Restraint stress augments postprandial gastric contractions but impairs antropyloric coordination in conscious rats[J].Am J Physiol Regul Integr Comp Physiol,2006,290(3):R616-R624.
[7]MING R,YU B,XU L,et al.Attenuation of stress-induced gastrointestinal motility disorder by gentiopicroside,from Gentiana macrophylla Pall[J].Fitoterapia,2015(103):265-276.
[8]LANDEIRA-FERNANDEZ J.Participation of NMDA receptors in the lateral hypothalamus in gastric erosion induced by cold-water restraint[J].Physiol Behav,2015(140):209-214.
[9]JOHAN D S.Physiology of the gastrointestinal tract[M].5th ed.Amsterdam:Elsevier Academic Press,2012:1979-2000.
[10]BROWNING K N,BABIC T,TOTI L,et al.Plasticity in the brainstem vagal circuits controlling gastric motor function triggered by corticotropin releasing factor[J].J Physiol,2014,592(20):4591-4605.
[11]JUNG Y S,KIM M Y,LEE H S,et al.Effect of DA-9701,a novel prokinetic agent,on stress-induced delayed gastric emptying and hormonal changes in rats[J].Neurogastroenterol Motil,2013,25(3):254-259,e166.
[12]COLLARES-BUZATO C B,COLLARES E F,FERNANDES G A.Effect of paraventricular nucleus lesion and cold restraint stress on gastric emptying of a liquid meal in rats[J].Braz J Med Biol Res,1993,26(9):1009-1014.
[13]MA S,MORILAK D A.Induction of Fos expression by acute immobilization stress is reduced in locus coeruleus and medial amygdala of wistar-kyoto rats compared to sprague-dawley rats[J].Neuroscience,2004,124(4):963-972.
[14]韩济生.神经科学原理:上[M].2版.北京:北京医科大学出版社,1999:410-417.
[15]ZHANG Y Y,CAO G H,ZHU W X,et al.Comparative study of cFos expression in rat dorsal vagal complex and nucleus ambiguus induced by different durations of restraint water-immersion stress[J].Chin J Physiol,2009,52(3):143-150.
[16]TAKAHASHI C,OHATA H,SHIBASAKI T.Corticotropin-releasing factor(CRF)receptor subtypes in mediating neuronal activation of brain areas involved in responses to intracerebroventricular CRF and stress in rats[J].Peptides,2011,32(12):2384-2393.
[17]BONAZ B,TACHE Y.Induction of Fos immunoreactivity in the rat brain after cold-restraint induced gastric lesions and fecal excretion[J].Brain Res,1994,652(1):56-64.
[18]宿长军,段丽,饶志仁.大鼠束缚后脑内Fos蛋白的表达[J].解剖学报,2005,36(2):123-126.
[19]PACK K,PALKOVITS M.Stressor specificity of central neuroendocrine responses:implications for stress-related disorders[J].Endocrine Reviews,2001,22(4):502-548.
[20]CRUZ M T,MURPHY E C,SAHIBZADA N,et al.A reevaluation of the effects of stimulation of the dorsal motor nucleus of the vagus on gastric motility in the rat[J].Am J Physiol Regul Integr Comp Physiol,2007,292(1):R291-R307.
[21]ZHOU S Y,LU Y X,YAO H R,et al.Spatial organization of neurons in the dorsal motor nucleus of the vagus synapsing with intragastric cholinergic and nitric oxide/VIP neurons in the rat[J].Am J Physiol Gastrointest Liver Physiol,2008,294(5):G1201-G1209.
[22]朱长庚.神经解剖学[M].2版.北京:人民卫生出版社,2009:454-459.
[23]PAXINOS G,WATSON C.The rat brain in stereotaxic coordinates[M].5th ed.Burlington:Elsevier Academic Press,2005.
[24]ZHAO D Q,AI H B.Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain[J].Plos One,2011,6(8):e233622.