迷走复合体和迷走神经在炎症反应中的可塑性
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摘要
为了探究炎症反应过程中孤束核-迷走神经背核-迷走神经的可塑性变化,实验分内毒素脂多糖(LPS)组和对照组2组,LPS组大鼠股静脉注射LPS,对照组大鼠股静脉注射同体积的生理盐水,测定迷走神经放电频率、血压、血清肿瘤坏死因子α(TNF-α)水平以及孤束核和迷走神经背核内Fos阳性神经元表达情况。结果表明:LPS注射后,迷走神经放电频率明显增大,血压虽有降低但不明显;与对照组相比,LPS组神经放电频率明显增大,血清TNF-α水平显著升高,孤束核和迷走神经背核的Fos表达均显著增强,说明迷走神经和孤束核、迷走神经背核参与了炎症反应。
To explore plasticity changes of the nucleus tractus solitarious-dorsal motor nucleus of vagus-vagus nerve pathway in inflammatory response,the experiment was divided into two groups of lipopolysaccharide( LPS) group and control group. The rats in LPS group were vena femoralis injected LPS and the rats in control group were vena femoralis injected saline of the same volume. The frequency of vagus nerve discharge,arterial pressure,tumor necrosis factor α( TNF-α)levels as well as Fos positive neurons express in the nucleus tractus solitarious and dorsal motor nucleus of vagus were measured. The results show that compared with before LPS injection,the discharge frequency of the vagus nerve increases significantly after LPS injection,and the blood pressure decreases but not significant. Comparing with the control group,the frequency of vagus nerve discharge,serum TNF-α level,Fos expressionofbrain stem of nucleus tractus solitarious,and dorsal motor nucleus of vagus solitary enhance significantly in LPS group,which shows that the vagus nerve,nucleus tractus solitarious,and the dorsal motor nucleus of the vagus are involved in the inflammatory response.
To explore plasticity changes of the nucleus tractus solitarious-dorsal motor nucleus of vagus-vagus nerve pathway in inflammatory response,the experiment was divided into two groups of lipopolysaccharide( LPS) group and control group. The rats in LPS group were vena femoralis injected LPS and the rats in control group were vena femoralis injected saline of the same volume. The frequency of vagus nerve discharge,arterial pressure,tumor necrosis factor α( TNF-α)levels as well as Fos positive neurons express in the nucleus tractus solitarious and dorsal motor nucleus of vagus were measured. The results show that compared with before LPS injection,the discharge frequency of the vagus nerve increases significantly after LPS injection,and the blood pressure decreases but not significant. Comparing with the control group,the frequency of vagus nerve discharge,serum TNF-α level,Fos expressionofbrain stem of nucleus tractus solitarious,and dorsal motor nucleus of vagus solitary enhance significantly in LPS group,which shows that the vagus nerve,nucleus tractus solitarious,and the dorsal motor nucleus of the vagus are involved in the inflammatory response.
引文
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[2]项水英,丛文娟,刘自兵.胆碱能抗炎通路及其在中医药抗炎效应中的研究进展[J].辽宁中医药大学学报,2018,20(2):140-143.
[3]HERMANN G E,EMCH G S,TOVAR C A,et a1.c-Fos generation in the dorsal vagal complex after systemic endotoxin is not dependent on the vagus nerve[J].Am J Physiol Regul Integr Comp Physiol,2001,280(1):289-299.
[4]黄健,杨志焕,陈力勇,等.全身炎症反应大鼠迷走神经反射通路兴奋性的改变[J].重庆医学,2008,37(1):55-57.
[5]包伦,徐贤坤,曾宪垠.大鼠股动、静脉插管手术方法的建立及应用[J].中国科技信息,2005(18):235-237,236.
[6]石德光,胡森,姜小国,等.电刺激迷走神经对内毒素血症所致急性肺损伤的影响[J].中国危重病急救医学,2002,14(12):732-735.
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[12]SCHORR E C,ARNASON B G.Interactions between the sympathetic nervous system and the immune system[J].Brain Behav Immun,1999,13(4):271-278.
[13]HALL D M,SUO J L,WEBER R J.Opioid mediated effects on the immune system:sympathetic nervous system involvement[J].Journal of Neuroimmunology,1998,83(1/2):29-35.
[14]JONAKAIT G M,HART R P.Immune cytokine regulation of sympathetic ganglion response to injury[J].Neuroimmunomodulation,1995,2(4):236-240.
[15]KAWASHIMA K,FUJII T.Extraneuronal cholinergic system in lymphocytes[J].Pharmacol Ther,2000,86(1):29-48.
[16]路秀英,杨贵贞,姜文,等.膈下迷走神经切断降低LPS所致大鼠发热及室旁核神经元放电频率[J].中国应用生理学杂志,2002,18(4):337-339.
[17]雷玉华,赵劲波,张长江,等.颈迷走神经刺激对大鼠心肌缺血再灌注损伤心肌氧化应激和细胞自噬的影响研究[J].心肺血管病杂志,2018,37(2):153-158,168.
[18]李冰梅,常娜,马静.迷走神经电刺激对内毒素血症小鼠肠道上皮细胞紧密连接及肺的血气屏障的影响[J].广东医学,2017,38(16):2439-2443.
[19]THOMAS R B,STEVEN G F,MAHENDAR O,et al.Pharmacological stimulation of the cholinergic antinflammatory pathway[J].J Exp Med,2002,195(6):781-788.
[20]SAGAR S M,PRICE K J,KASTING N W,et al.Anatomic patterns of Fosimmunostaining in rat brain following systemic endotoxin administration[J].Brain Res Bull,1995,36(4):381-392.
[21]YOKOYAMA C,SASAKI K.Regional expressions of Fos-like immunoreactivity in rat cerebral cortex after stress:restraint and intraperitoneal lipopolysaccharide[J].Brain Res,1999,816(2):267-275.
[22]ELMQUIST J K,ACKERMANN M R,REGISTER K B,et al.Induction of Fos-like immunoreactivity in the rat brain following Pasteurella multocida endotoxin administration[J].Endocrinology,1993,133(6):3054-3057.
[23]韩秀引,王蕾,马玉琼,等.LPS对大鼠下丘脑视上核n NOS和c-fos表达的影响[J].神经解剖学杂志,2004,20(5):475-478.
[2]项水英,丛文娟,刘自兵.胆碱能抗炎通路及其在中医药抗炎效应中的研究进展[J].辽宁中医药大学学报,2018,20(2):140-143.
[3]HERMANN G E,EMCH G S,TOVAR C A,et a1.c-Fos generation in the dorsal vagal complex after systemic endotoxin is not dependent on the vagus nerve[J].Am J Physiol Regul Integr Comp Physiol,2001,280(1):289-299.
[4]黄健,杨志焕,陈力勇,等.全身炎症反应大鼠迷走神经反射通路兴奋性的改变[J].重庆医学,2008,37(1):55-57.
[5]包伦,徐贤坤,曾宪垠.大鼠股动、静脉插管手术方法的建立及应用[J].中国科技信息,2005(18):235-237,236.
[6]石德光,胡森,姜小国,等.电刺激迷走神经对内毒素血症所致急性肺损伤的影响[J].中国危重病急救医学,2002,14(12):732-735.
[7]MAIER S F.Bi-directional immunebrain communication:implications for understanding stress,pain,and cognition[J].2003,17(2):69-85.
[8]盛志勇,胡森.多器官功能障碍综合征[M].北京:科学出版社,1999:49-58.
[9]石德光,杜欣,胡森,等.电刺激迷走神经对内毒素休克大鼠血压的影响[J].中华医院感染学杂志,2004,14(11):1249-1250.
[10]MADDEN K S.Catecholamines,sympathetic inervation,and immunity[J].Brain Behav Immun,2003,17(11):5-10.
[11]ELENKOV I J,WILDER R L,CHROUSOS G P,et al.The sympathetic nerve:an integrative interface between two supersystems:the brain and the immune system[J].Pharmacol Rev,2000,52(4):595-638.
[12]SCHORR E C,ARNASON B G.Interactions between the sympathetic nervous system and the immune system[J].Brain Behav Immun,1999,13(4):271-278.
[13]HALL D M,SUO J L,WEBER R J.Opioid mediated effects on the immune system:sympathetic nervous system involvement[J].Journal of Neuroimmunology,1998,83(1/2):29-35.
[14]JONAKAIT G M,HART R P.Immune cytokine regulation of sympathetic ganglion response to injury[J].Neuroimmunomodulation,1995,2(4):236-240.
[15]KAWASHIMA K,FUJII T.Extraneuronal cholinergic system in lymphocytes[J].Pharmacol Ther,2000,86(1):29-48.
[16]路秀英,杨贵贞,姜文,等.膈下迷走神经切断降低LPS所致大鼠发热及室旁核神经元放电频率[J].中国应用生理学杂志,2002,18(4):337-339.
[17]雷玉华,赵劲波,张长江,等.颈迷走神经刺激对大鼠心肌缺血再灌注损伤心肌氧化应激和细胞自噬的影响研究[J].心肺血管病杂志,2018,37(2):153-158,168.
[18]李冰梅,常娜,马静.迷走神经电刺激对内毒素血症小鼠肠道上皮细胞紧密连接及肺的血气屏障的影响[J].广东医学,2017,38(16):2439-2443.
[19]THOMAS R B,STEVEN G F,MAHENDAR O,et al.Pharmacological stimulation of the cholinergic antinflammatory pathway[J].J Exp Med,2002,195(6):781-788.
[20]SAGAR S M,PRICE K J,KASTING N W,et al.Anatomic patterns of Fosimmunostaining in rat brain following systemic endotoxin administration[J].Brain Res Bull,1995,36(4):381-392.
[21]YOKOYAMA C,SASAKI K.Regional expressions of Fos-like immunoreactivity in rat cerebral cortex after stress:restraint and intraperitoneal lipopolysaccharide[J].Brain Res,1999,816(2):267-275.
[22]ELMQUIST J K,ACKERMANN M R,REGISTER K B,et al.Induction of Fos-like immunoreactivity in the rat brain following Pasteurella multocida endotoxin administration[J].Endocrinology,1993,133(6):3054-3057.
[23]韩秀引,王蕾,马玉琼,等.LPS对大鼠下丘脑视上核n NOS和c-fos表达的影响[J].神经解剖学杂志,2004,20(5):475-478.