犬急性阈上迷走神经刺激介导型心房纤颤模型心房重构的变化
|
摘要
目的对比2种非瓣膜病变急性房颤模型中心房肌电生理、结构指标、神经指标的变化,了解最佳模拟急性房颤模型心房重构的方法。方法 13只比格犬,随机分为2组,GroupI为心房快速起搏(rapid atria pacing,RAP)6h组,GroupII为RAP+迷走神经刺激(vagal nerve stimulation,VNS)6h组,2组均在1、3、6h后给予测量心房肌单相动作电位(monophasic action potential,MAP)、房颤诱发率;实验结束后取标本测量2组马松染色(MASSON染色)、糖原染色(PAS染色)、神经指标的变化。结果 2组心房肌MAP随着心房快速起搏时间的延长,出现振幅减小、时限缩短的趋势。2组房颤诱发率以GroupII为高。2组心房糖原染色、神经丛中酪氨酸羟化酶(Tyrosine hydroxylase,TH)和胆碱乙酰转氨酶(Choline acetyl transaminase,CHAT)染色和定量差异均有统计学意义(P均<0.05)。结论阈上迷走神经刺激的介入加剧心房重构的恶化,使用心房快速起搏+迷走神经刺激建立的房颤模型成功率高、周期短、重复性强,同时也为研究迷走神经在房颤发病及治疗中的地位和作用提供了重要的研究基础。
Objective To compare the changes of atrial remodeling values in the two types of acute atrial fibrillation(AF)models and thereby discuss the best models to imitate acute AF.Methods Thirteen adult Beagles were randomly allotted to two groups,Group I(n =6)underwent 6 hrapid atrial pacing(RAP)only;Group II(n =7)underwent 6 hvagal nerve stimulation(VNS)plus RAP.Monophasic action potential(MAP),AF induction rate and other remodeling values were assessed.Results The MAP of atrial muscle extended along with the time,action potential duration(APD)occurred and there was a significant tendency of APD90 shrinking.Nevertheless,the inducing rate of AF in the Group II interference was higher in these two groups.Abnormal results were found in the two groups of atrial glycogen staining,tyrosine hydroxylase(TH)and choline acetyl transaminase(CHAT)staining and quantitative analysis of ganglionic plexus.Conclusion The AF model established on the basis of VNS+RAP is more reliable with high success rate and strong repeatability.At the same time,for the study of the vagal nerve in the role of the pathogenesis and treatment of AF,it provides important studying foundation.
Objective To compare the changes of atrial remodeling values in the two types of acute atrial fibrillation(AF)models and thereby discuss the best models to imitate acute AF.Methods Thirteen adult Beagles were randomly allotted to two groups,Group I(n =6)underwent 6 hrapid atrial pacing(RAP)only;Group II(n =7)underwent 6 hvagal nerve stimulation(VNS)plus RAP.Monophasic action potential(MAP),AF induction rate and other remodeling values were assessed.Results The MAP of atrial muscle extended along with the time,action potential duration(APD)occurred and there was a significant tendency of APD90 shrinking.Nevertheless,the inducing rate of AF in the Group II interference was higher in these two groups.Abnormal results were found in the two groups of atrial glycogen staining,tyrosine hydroxylase(TH)and choline acetyl transaminase(CHAT)staining and quantitative analysis of ganglionic plexus.Conclusion The AF model established on the basis of VNS+RAP is more reliable with high success rate and strong repeatability.At the same time,for the study of the vagal nerve in the role of the pathogenesis and treatment of AF,it provides important studying foundation.
引文
[1]SCHNABEL R B,YIN X,GONA P,et al.5 0year trends in atrial fibrillation prevalence,incidence,risk factors,and mortality in the Framingham Heart Study:a cohort study[J].Lancet,2015,386(9989):154-162.
[2]ROGERS P A,BERNARD M L,MADIAS C,et al.Current evidence-based understanding of the epidemiology,prevention,and treatment of atrial fibrillation[J].Curr Probl Cardiol,2018,43(6):241-283.
[3]NATTEL S.Therapeutic implications of atrial fibrillation mechanisms:can mechanistic insights be used to improve AF management[J].Cardiovasc Res,2002,54(2):347-360.
[4]MORILLO C A,KELEIN G J,JONES D L,et al.Chronic rapid atrial pacing.structural,functional,and electrophysiological characteristics of a new model of sustained atrial fibrillation[J].Circulation,1995,91(5):1588-1595.
[5]RASU A,JACQUEMET V,VESIN J M,et al.Influence of atrial substrate on local capture induced by rapid pacing of atrial fibrillation[J].Europace,2014,16(5):766-773.
[6]SHI Y,DUCHARME A,LI D,et al.Remodeling of atrial dimensions and emptying function in canine models of atrial fibrillation[J].Cardiovasc Res,2001,52(2):217-225.
[7]WIJFFELS M C,KIRCHHOF C J,DORLAND R,et al.Atrial fibrillation begets atrial fibrillation:a study in awake chronically instrumented goats[J].Circulation,1995,92(7):1954-1968.
[8]ACHOU C C,CHEN P S.New concepts in atrial fibrillation:mechanism and remodeling[J].Med Clin North Am,2008,92(1):53-63.
[9]NATTEL S,HARADA M.Atrial remodeling and atrial fibrillation:recent advances and translational perspectives[J].J Am Coll Cardiol,2014,63(22):2335-2345.
[10]VERHEULE S,WILSON E,BANTHIA S,et al.Directiondependent conduction abnormalities in a canine model of atrial fibrillation due to chronic atrial dilatation[J].Am J Physiol Heart Circ Physiol,2004,287(2):634-644.
[11]SHARIFOV O F,FEDOROV V V,BELOSHAPKO G G,et al.Roles of adrenergic and cholinergic stimulation in spontaneous atrial fibrillation in dogs[J].J Am Coll Cardiol,2004,43(3):483-490.
[12]YU L,LI X,HUANG B,et al.Atrial fibrillation in acute obstructive sleep apnea:autonomic nervous mechanism and modulation[J].J Am Heart Assoc,2017,6(9):e006264.
[13]TAKEMOTO Y,RAMIREZ R J,KAUR K,et al.Eplerenone reduces atrial fibrillation burden without preventing atrial electrical remodeling[J].J Am Coll Cardiol,2017,70(23):2893-2905.
[14]CHEN P S,TAN A Y.Autonomic nerve activity and atrial fibrillation[J].Heart Rhythm,2007,4(3Suppl):S61-64.
[15]储睿,殷瑛,周兴宇,等.雷诺定受体2在抑郁大鼠心房颤动中的作用[J].心脏起搏与心电生理杂志,2018,2(32):152-156.
[2]ROGERS P A,BERNARD M L,MADIAS C,et al.Current evidence-based understanding of the epidemiology,prevention,and treatment of atrial fibrillation[J].Curr Probl Cardiol,2018,43(6):241-283.
[3]NATTEL S.Therapeutic implications of atrial fibrillation mechanisms:can mechanistic insights be used to improve AF management[J].Cardiovasc Res,2002,54(2):347-360.
[4]MORILLO C A,KELEIN G J,JONES D L,et al.Chronic rapid atrial pacing.structural,functional,and electrophysiological characteristics of a new model of sustained atrial fibrillation[J].Circulation,1995,91(5):1588-1595.
[5]RASU A,JACQUEMET V,VESIN J M,et al.Influence of atrial substrate on local capture induced by rapid pacing of atrial fibrillation[J].Europace,2014,16(5):766-773.
[6]SHI Y,DUCHARME A,LI D,et al.Remodeling of atrial dimensions and emptying function in canine models of atrial fibrillation[J].Cardiovasc Res,2001,52(2):217-225.
[7]WIJFFELS M C,KIRCHHOF C J,DORLAND R,et al.Atrial fibrillation begets atrial fibrillation:a study in awake chronically instrumented goats[J].Circulation,1995,92(7):1954-1968.
[8]ACHOU C C,CHEN P S.New concepts in atrial fibrillation:mechanism and remodeling[J].Med Clin North Am,2008,92(1):53-63.
[9]NATTEL S,HARADA M.Atrial remodeling and atrial fibrillation:recent advances and translational perspectives[J].J Am Coll Cardiol,2014,63(22):2335-2345.
[10]VERHEULE S,WILSON E,BANTHIA S,et al.Directiondependent conduction abnormalities in a canine model of atrial fibrillation due to chronic atrial dilatation[J].Am J Physiol Heart Circ Physiol,2004,287(2):634-644.
[11]SHARIFOV O F,FEDOROV V V,BELOSHAPKO G G,et al.Roles of adrenergic and cholinergic stimulation in spontaneous atrial fibrillation in dogs[J].J Am Coll Cardiol,2004,43(3):483-490.
[12]YU L,LI X,HUANG B,et al.Atrial fibrillation in acute obstructive sleep apnea:autonomic nervous mechanism and modulation[J].J Am Heart Assoc,2017,6(9):e006264.
[13]TAKEMOTO Y,RAMIREZ R J,KAUR K,et al.Eplerenone reduces atrial fibrillation burden without preventing atrial electrical remodeling[J].J Am Coll Cardiol,2017,70(23):2893-2905.
[14]CHEN P S,TAN A Y.Autonomic nerve activity and atrial fibrillation[J].Heart Rhythm,2007,4(3Suppl):S61-64.
[15]储睿,殷瑛,周兴宇,等.雷诺定受体2在抑郁大鼠心房颤动中的作用[J].心脏起搏与心电生理杂志,2018,2(32):152-156.