基于核酸适配子的CdTe发光量子点标记赭曲霉毒素A检测新方法研究
|
摘要
将赭曲霉毒素A(OTA)适配子与Fe3O4磁性纳米粒子结合,CdTe发光量子点标记于OTA适配子互补短链上,结合荧光分析技术、适配子识别技术与磁分离富集技术,建立一种OTA的高特异性和灵敏性的新型检测技术。
CdTe luminescent quantum dots(QDs) were labeled on the complementary short chains of ochratoxin A(OTA) aptamers by binding OTA aptamers to Fe_3 O_4 magnetic nanoparticles. A novel OTA detection technique with high specificity and sensitivity was developed by combining fluorescence analysis, aptamer recognition and magnetic separation and enrichment techniques.
CdTe luminescent quantum dots(QDs) were labeled on the complementary short chains of ochratoxin A(OTA) aptamers by binding OTA aptamers to Fe_3 O_4 magnetic nanoparticles. A novel OTA detection technique with high specificity and sensitivity was developed by combining fluorescence analysis, aptamer recognition and magnetic separation and enrichment techniques.
引文
[1]B R I A NM,M AT T O U S S IH,G O L D M A NER,e ta l.Preparationofquantumdot-biotinconjugatesandtheirusein immunochromatographyassays[J]. AnalylicalChemistry,2003,75(16):4043-4049.
[2]WANGLBAOJ,LUNWP,etal.One-potsynthesisand bioapplicationofamine-functionalizedmagnetitenanoparticles and hollow nanospheres[J]. Chemistry-A European Journal, 2006,12(24):6341-6347.
[3]DENGH,LIX,PENGQ,etal.Monodispersemagneticsinglecrystalferritemicrospheres[J]. AngewandteChemieIntemation Edition, 2005, 44(18):2782-2785.
[4] HENGLEIN A. Small-particle research:Physicochemical properties of extremely small colloidal metal and semiconductor particles[J].Cheminform, 1990, 21(17):1861-1873.
[5]李太山,刘绍璞,刘忠芳,等.碲化镉纳米晶溶液的荧光和共振瑞利散射特性及碲化镉纳米晶与氨基糖苷类抗生素相互作用[J].中国科学, 2008, 38(9):798-807.
[6]ZHANGH,ZHOUZ, YANGB,etal.Theinfluenceofcarboxyl groupsonthephotoluminescenceofmercaptocarboxylicacidstabilized CdTe nanoparticles[J]. Journal ofPhysical Chemistry B,2003, 107(1):8-13.
[7]刘兵波,刘旭辉,李德娜,等.复色量子点的制备与免疫分析应用[J].科学通报.2007, 52(24):2846-2851.
[2]WANGLBAOJ,LUNWP,etal.One-potsynthesisand bioapplicationofamine-functionalizedmagnetitenanoparticles and hollow nanospheres[J]. Chemistry-A European Journal, 2006,12(24):6341-6347.
[3]DENGH,LIX,PENGQ,etal.Monodispersemagneticsinglecrystalferritemicrospheres[J]. AngewandteChemieIntemation Edition, 2005, 44(18):2782-2785.
[4] HENGLEIN A. Small-particle research:Physicochemical properties of extremely small colloidal metal and semiconductor particles[J].Cheminform, 1990, 21(17):1861-1873.
[5]李太山,刘绍璞,刘忠芳,等.碲化镉纳米晶溶液的荧光和共振瑞利散射特性及碲化镉纳米晶与氨基糖苷类抗生素相互作用[J].中国科学, 2008, 38(9):798-807.
[6]ZHANGH,ZHOUZ, YANGB,etal.Theinfluenceofcarboxyl groupsonthephotoluminescenceofmercaptocarboxylicacidstabilized CdTe nanoparticles[J]. Journal ofPhysical Chemistry B,2003, 107(1):8-13.
[7]刘兵波,刘旭辉,李德娜,等.复色量子点的制备与免疫分析应用[J].科学通报.2007, 52(24):2846-2851.