大鼠与鲫鱼肠道内含物中微生物菌群的研究
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摘要
目的对生活环境差异较大的动物肠道内含物中微生物菌群分布规律进行研究。方法通过提取SD大鼠与鲫鱼不同肠段的内含物菌液,用不同的选择性培养基进行分离培养,鉴定后进行平板计数。结果大鼠肠道中乳酸杆菌所占比重高于鲫鱼肠道,其余菌落(双歧杆菌、肠球菌、大肠杆菌)比重明显小于鲫鱼肠道;大鼠和鲫鱼肠道厌氧的双歧杆菌沿肠道向下数量递增。需氧的肠球菌在鲫鱼、大鼠整个肠道中的数量级相当。结论研究不同动物肠道的微生物信息可以为病理学、毒理学的研究提供理论信息。
Objective The distribution of intestinal microflora in different parts of animals,intestine which living environment are quite different. Method With taking the contents of different intestinal segments of mice and crucian carp, extracting and culturing with different selective medium, and then carring out the plate count. Result It was found that the proportion of Lactobacillus in the intestine of rats was higher than that of crucian carp, and the proportion of other colonies(Bifidobacterium, Enterococcus, Escherichia coli) was significantly smaller than that of crucian carp. The distribution of intestinal anaerobic Bifidobacterium in rats and crucian carp increase in the downward direction along the gut.The magnitude of aerobic Enterococcus in the intestine of rats and crucian carp was almost same. Conclusion The inestinal microbiological information of different animals can preovide some experimental basis for the study of pathology and toxicology.
Objective The distribution of intestinal microflora in different parts of animals,intestine which living environment are quite different. Method With taking the contents of different intestinal segments of mice and crucian carp, extracting and culturing with different selective medium, and then carring out the plate count. Result It was found that the proportion of Lactobacillus in the intestine of rats was higher than that of crucian carp, and the proportion of other colonies(Bifidobacterium, Enterococcus, Escherichia coli) was significantly smaller than that of crucian carp. The distribution of intestinal anaerobic Bifidobacterium in rats and crucian carp increase in the downward direction along the gut.The magnitude of aerobic Enterococcus in the intestine of rats and crucian carp was almost same. Conclusion The inestinal microbiological information of different animals can preovide some experimental basis for the study of pathology and toxicology.
引文
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[12] Liu J, Wu D, Ahmed A, et al. Comparison of the gut microbe profiles and numbers between patients with liver cirrhosis and healthy individuals[J]. Curr Microbio, 2012, 65(1):7-13.
[13] Tian Y, Zhang L, Wang Y, et al. Age-related topographical metabolic signatures for the rat gastrointestinal contents.[J]. J Proteome Res, 2012, 11(2):1397-411.
[14] Goel A, Gupta M, Aggarwal R. Gut microbiota and liver disease[J].J Gastroenterol Hepatol,2014, 29(6):1139-1148.
[15] Mei W, Ahrné S, Jeppsson B, et al. Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes[J].Fems microbiol Ecol,2005, 54(2):219-231.
[16] Hagi T, Tanaka D, Iwamura Y, et al. Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish.[J]. Aquaculture, 2004, 234(1-4):335-346.
[17] 尹军霞, 陈瑛, 孟丽丽. 益生菌剂对鲫鱼肠道菌群影响的初步研究[J]. 水产科学, 2007, 26(11):610-612.
[2] 魏晓, 刘威, 袁静,等. 人类肠道菌群与疾病关系的元基因组学研究进展[J]. 中国微生态学杂志, 2011,23(1):75-80.
[3] Zoetendal E G, Rajilic-Stojanovic M, De-Vos W M. High-throughput diversity and functionality analysis of the gas-trointestinal tract microbiota [J]. Gut, 2008, 57(11): 1605-1615.
[4] 翟齐啸, 田丰伟, 王刚,等. 肠道微生物与人体健康的研究进展[J]. 食品科学, 2013, 34(15):337-341.
[5] Liu J, Wu D, Ahmed A, et al. Comparison of the Gut Microbe Profiles and Numbers Between Patients with Liver Cirrhosis and Healthy Individuals[J].Curr Microbiol, 2012, 65(1):7-13.
[6] 陈恒, 叶盛, 左延文,等. 肠道微生物与肥胖关系研究进展[J]. 现代预防医学, 2008, 35(4):608-609.
[7] 王腊梅, 孔祥阳, 王昆华. 肠道菌群与艾滋病关系的研究进展[J]. 细胞与分子免疫学杂志, 2016, 32(6):859-862.
[8] 蔡林彬, 付轶群, 李明阳,等. 肠道微生物群与精神疾病的关系[J]. 国际生物制品学杂志, 2014,37(5):232-237.
[9] 梁效, 彭喜春. 饮食、肠道微生物与人体健康[J]. 肠外与肠内营养, 2011, 18(5):304-306.
[10] 强华, 王耿夏, 谢蔓凌. 卡那霉素ELB选择培养基分离肠道肠球菌[J]. 中国卫生检验杂志, 2003, 13(2):145-145.
[11] 蒋婕, 郭抗萧, 周赛男,等. 一种提取小鼠肠道中活体微生物的方法[J]. 中国微生态学杂志, 2013, 25(5):512-515.
[12] Liu J, Wu D, Ahmed A, et al. Comparison of the gut microbe profiles and numbers between patients with liver cirrhosis and healthy individuals[J]. Curr Microbio, 2012, 65(1):7-13.
[13] Tian Y, Zhang L, Wang Y, et al. Age-related topographical metabolic signatures for the rat gastrointestinal contents.[J]. J Proteome Res, 2012, 11(2):1397-411.
[14] Goel A, Gupta M, Aggarwal R. Gut microbiota and liver disease[J].J Gastroenterol Hepatol,2014, 29(6):1139-1148.
[15] Mei W, Ahrné S, Jeppsson B, et al. Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes[J].Fems microbiol Ecol,2005, 54(2):219-231.
[16] Hagi T, Tanaka D, Iwamura Y, et al. Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish.[J]. Aquaculture, 2004, 234(1-4):335-346.
[17] 尹军霞, 陈瑛, 孟丽丽. 益生菌剂对鲫鱼肠道菌群影响的初步研究[J]. 水产科学, 2007, 26(11):610-612.