电子束对液体透明质酸分子量及抗氧化性的影响
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摘要
为了探明辐照对降解透明质酸理化特性的影响,采用电子束对0.5g·100mL-1透明质酸氯化钠溶液进行辐照处理,测定透明质酸辐照前后分子量、黏度特性、pH值及抗氧化性。结果表明:透明质酸的分子量、黏度特性、pH值随辐照剂量的增加而降低;对羟自由基(·OH)及超氧阴离子自由基(O2·-)清除作用随着剂量的增大逐渐减弱,对DPPH·自由基清除作用和还原能力随着剂量的增大逐渐增强;辐照前后透明质酸外观品质没有明显的变化,流动性增强。本试验对拓宽透明质酸的应用领域具有重要的意义。
Hyaluronic acid Sodium Chloride Solution of 0. 5 g·( 100 m L)- 1was irradiated by The electron beam,which weight,viscosity,p H value and oxidation resistance were measured before and after irradiation. The results showed that the molecular weight,the viscosity,p H value of hyaluronic acid were decreased with the increase of irradiation dose;scavenging effect of the hydroxyl free radical( ·OH) and superoxide anion free radical( O2·-) were gradually weakened with the dose increasing; The DPPH free radical scavenging activity and reducing power were increased as the dose increasing; hyaluronic acid appearance quality did not change obviously before and after irradiation,which liquidity was enhanced. It has important significance to broaden the application fields of hyaluronic acid.
Hyaluronic acid Sodium Chloride Solution of 0. 5 g·( 100 m L)- 1was irradiated by The electron beam,which weight,viscosity,p H value and oxidation resistance were measured before and after irradiation. The results showed that the molecular weight,the viscosity,p H value of hyaluronic acid were decreased with the increase of irradiation dose;scavenging effect of the hydroxyl free radical( ·OH) and superoxide anion free radical( O2·-) were gradually weakened with the dose increasing; The DPPH free radical scavenging activity and reducing power were increased as the dose increasing; hyaluronic acid appearance quality did not change obviously before and after irradiation,which liquidity was enhanced. It has important significance to broaden the application fields of hyaluronic acid.
引文
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[21]高春燕,田呈瑞,周默.枸杞多糖体清除自由基活性研究[J].三峡大学学报,2005,27(5):23-26
[22]李顺峰,张丽华,付娟妮,刘兴华.真姬菇子实体多糖体外抗氧化特性研究[J].西北农业学报,2008,17(4):302-305
[23]包玉威,罗兴武,江海艳,陈娜,程超.鸭跖草水溶性多糖的提取纯化及纯化前后抗氧化性能研究[J].现代食品科技,2008,24(9):880-882
[24]程超,李伟,汪兴平.平菇水溶液多糖结构表征与抗氧化作用[J].食品科学,2005,26(8):55-57
[25]邹朝晖,王强,邓钢桥,李淑荣,高美须.辐照对透明质酸抗氧化性及结构特性的影响[J].核农学报,2011,25(1):83-87
[2]Schiraldi C,Gatta A L,Ross M D.Biotcchnological production and application of hyaluronan[EB/OL].2012,http://www.intechopen.com/download/pdf/12157
[3]Liu Y C,Shu X Z,Prestwich G D.Biocompatibility and stability of disulfide-crosslinked hyaluronan films[J].Biomaterials,2005,26(23):4737-4746
[4]张文强,黄岳山,支晓兴.透明质酸在临床医学中的应用[J].中国组织工程研究与临床康复,2008,12(23):4515-4518
[5]Guechot J,Edrief S,Lasnier E,Kuper R Automated assay of hyaluronic acid in serum[J].Immuno-analyse Biologie Specialisee,2008,23(3):148-152
[6]赵玲.生物可降解材料在眼科临床中的应用[J].中国组织工程研究与临床康复,2011,15(51):9659-9662
[7]郑松柏,付德生.透明质酸钠治疗膝关节骨性关节炎60例分析[J].中国误诊杂志,2009,9(34):84-86
[8]凌沛学,张天民,李琦,荣晓花.玻璃酸研究与应用[M].北京:人民卫生出版社,2010:1-6
[9]孙嘉阳,秦华东,石铁峰,刘垚.几丁糖与透明质酸钠预防甲状腺术后粘连效果的对比研究[J].现代医学,2010,38(5):481-484
[10]Necas J,Bartosikova L,Brauner P,Kolar J.Hyaluronic acid(hyaluronan):a review[J].Veterinarni Medicina,2008,53(8):397-411
[11]何朝晖.高品质透明质酸在化妆品中的应用[J].中国高新技术企业,2013,251(8):251-252.
[12]宋磊,王腾飞.透明质酸的研究现状综述[J].山东轻工业学院学报,2012,26(2):15-18
[13]Tian W M,Zhang C,Hou S P,Yu X.Hyaluronic acid hydrogel as Nogo-66 receptor antibody delivery system forthe repairing of injured rat brain:in vitro[J].Journal of Controlled Release,2005,102(1):13-25
[14]陈伟,罗曼,徐永根,闵宗英.透明质酸钠在用丝裂霉素C的小梁切除术中的应用[J].中国实用眼科杂志,2008,26(12):1347-1349
[15]Choi K Y,Lee S,Park K,Kim K,Park J H,Kwon L C.Preparation and characterization of hyaluronic acid-based hydrogel nanoparticles[J].Journal of Physics and Chemistry of Solids,2008,69(5/6):1591-1595
[16]Elisabetta E,Enea M,Rita C.Hyaluronan-based microspheres as tools for drug delivery:a comparative study[J].International Journal of Clinical Pharmacology and Therapeutics,2005,288(1):35-49
[17]Zhang X,Duan X J,Tan W S.Mechanism for the effect of agitation on the molecular weight of hyaluronic aci produced by Steptococcus zooepidemicus[J].Food Chemistry,2010,119(4):1643-1646
[18]Marguerite R.Main properties and current application of some polysaccharides as biomaterials[J].Polymer International,2007,57(3):397-430
[19]陈奕涵,叶锐,钱悦,管世敏生物法制取透明质酸的研究进展[J].上海应用技术学院学报,2012,12(2):147-151
[20]冯建成,冯浩,牛成,范春蕾.高分子量透明质酸生产菌SH0201的诱变选育[J].中国酿造,2011,228(3):45-47
[21]高春燕,田呈瑞,周默.枸杞多糖体清除自由基活性研究[J].三峡大学学报,2005,27(5):23-26
[22]李顺峰,张丽华,付娟妮,刘兴华.真姬菇子实体多糖体外抗氧化特性研究[J].西北农业学报,2008,17(4):302-305
[23]包玉威,罗兴武,江海艳,陈娜,程超.鸭跖草水溶性多糖的提取纯化及纯化前后抗氧化性能研究[J].现代食品科技,2008,24(9):880-882
[24]程超,李伟,汪兴平.平菇水溶液多糖结构表征与抗氧化作用[J].食品科学,2005,26(8):55-57
[25]邹朝晖,王强,邓钢桥,李淑荣,高美须.辐照对透明质酸抗氧化性及结构特性的影响[J].核农学报,2011,25(1):83-87