摘要
为了探明辐照对降解透明质酸理化特性的影响,采用电子束对0.5g·100mL-1透明质酸氯化钠溶液进行辐照处理,测定透明质酸辐照前后分子量、黏度特性、pH值及抗氧化性。结果表明:透明质酸的分子量、黏度特性、pH值随辐照剂量的增加而降低;对羟自由基(·OH)及超氧阴离子自由基(O2·-)清除作用随着剂量的增大逐渐减弱,对DPPH·自由基清除作用和还原能力随着剂量的增大逐渐增强;辐照前后透明质酸外观品质没有明显的变化,流动性增强。本试验对拓宽透明质酸的应用领域具有重要的意义。
Hyaluronic acid Sodium Chloride Solution of 0. 5 g·( 100 m L)- 1was irradiated by The electron beam,which weight,viscosity,p H value and oxidation resistance were measured before and after irradiation. The results showed that the molecular weight,the viscosity,p H value of hyaluronic acid were decreased with the increase of irradiation dose;scavenging effect of the hydroxyl free radical( ·OH) and superoxide anion free radical( O2·-) were gradually weakened with the dose increasing; The DPPH free radical scavenging activity and reducing power were increased as the dose increasing; hyaluronic acid appearance quality did not change obviously before and after irradiation,which liquidity was enhanced. It has important significance to broaden the application fields of hyaluronic acid.
引文
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