鲤疱疹病毒2型研究进展
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摘要
鲤疱疹病毒2型(Cyprinid herpesvirus 2,CyHV-2)是引起鲫和金鱼造血器官坏死病的病原,在世界多个国家传播、流行,给鲫、金鱼和异育银鲫养殖业造成巨大经济损失。本文简要综述了鲤疱疹病毒2型的病原学、流行病学、诊断以及防控等研究进展,以期为鲤疱疹病毒2型基础研究和疾病防控策略提供参考。
Cyprinid herpesvirus 2(CyHV-2) as a causative virus of haematopoietic necrosis disease of goldfish and Carassius auratus has great harm to the breeding of goldfish and Carassius auratus gibelio in China and spread rapidly in many countries,causing enormous economic losses of goldfish and crucian carp farming. This review summarizes recent advances on Cy HV-2 research including pathogeny, epidemiology, diagnosis, and prevention and control of CyHV-2.
Cyprinid herpesvirus 2(CyHV-2) as a causative virus of haematopoietic necrosis disease of goldfish and Carassius auratus has great harm to the breeding of goldfish and Carassius auratus gibelio in China and spread rapidly in many countries,causing enormous economic losses of goldfish and crucian carp farming. This review summarizes recent advances on Cy HV-2 research including pathogeny, epidemiology, diagnosis, and prevention and control of CyHV-2.
引文
[1]Jung S J,Miyazaki T.Herpesviral haematopoietic necrosis of goldfish,Carassius auratus(L.)[J].Journal of Fish Diseases,1995,18(3):211-220.
[2]Waltzek T B,Kelley G O,Stone D M,et al.Koi herpesvirus representsathirdcyprinidherpesvirus(CyHV-3)inthefamily Herpesviridae[J].J Gen Virol,2005,86(6):1659-1667.
[3]Davision A J,Eberle R,Ehlers B,et al.The order Herpesvirales[J].Arch Virol,2009,154(1):171-177.
[4]Hanson L,Dishon A,Kotler M.Herpesviruses that infect fish viruses[J].Viruses,2011,3(11):2160-2191.
[5]Doszpoly A,Benko M,Csaba G,et al.Introduction of the Family Alloherpesviridae:the first molecular detection of herpesviruses of cyprinid fish in Hungary[J].Magyar Allatorvosok Lapja,2011,133(3):174-181.
[6]Xu J,Zeng L B,Zhang H,et al.Cyprinid herpesvirus 2 infection emerged in cultured gibel carp,Carassius gibelio in China[J].Veterinary Microbiology,2013,166(1-2):138-144.
[7]Groff J M,La Patra S E,Munn R J,et al.A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology[J].J Vet Diagn Invest,1998,10(4):375-378.
[8]Li L J,Luo Y Z,Gao Z X,et al.Molecular characterisation and prevalence of a new genotype of Cyprinid herpesvirus 2in mainland China[J].Can.J.Microbiol,2015,61(6):381-387.
[9]Davlson A J,Kurobe T,Gatherer D,et al.Comparative genomics of carp herpesviruses[J].Journal of Virology,2013,87(5):2908-2922.
[10]Zhou Y,Jiang N,Ma J,et al.Protective immunity in gibel carp,Carassius gibelio of the truncated proteins of cyprinid herpesvirus 2 expressed in Pichia pastoris[J].Fish&Shellfish Immunology,2015,47(2):1024-1031.
[11]Du M,Chen M L,Shen H F,et al.CyHV-2 ORF104 activates the p38 MAPK pathway[J].Fish&Shellfish Immunology,2015,46(2):268-273.
[12]Chang P H,Lee S H,Chiang H C,et al.Epizootic of herpes-like virus infection in goldfish,Carassius auratus in Taiwan[J].Fish Pathology,1999,34(4):209-210.
[13]Groff J M,La Patra S E,Munn R J,et al.A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology[J].J Vet Diagn Invest,1998,10(4):375-378[g1].
[14]Stephens F J,Raidal S R,Jones B.Haematopoietic necrosis in a goldfish(Carassius auratus)associated with an agent morphologically similar to herpesvirus[J].Australian Veterinary Journal,2004,82(3):167-169.
[15]Hine P M,Tham K M,Morrison R,et al.Cyprinid herpersvirus 2 in New Zealand goldfish[J].Surveillance,2006,33(4):3-5.
[16]Jeffery K R,Bateman K,Bayley A,et al.Isolation of a cyprinid herpesvirus 2 from goldfish Carassius auratus(L.),in the UK[J].J Fish Dis,2007,30(11):649-656.
[17]Doszpoly A,Benko M,Csaba G,et al.Introduction of the family Alloherpesviridae:the first molecular detection of herpesviruses of cyprinid fish in Hungary[J].Magyar Allatorvosok Lapja,2011,133(3):174-181.
[18]Wu T,Ding Z,Ren M,et al.The histo-and ultra-pathological studies on a fatal disease of Prussian carp(Carassius auratus)in mainland China associated with cyprinid herpesvirus 2(CyHV-2)[J].Aquaculture,2013,412-413(6):8-13.
[19]Daněk T,Kalous L,Vesel T,et al.Massive mortality of Prussian carp Carassius gibelio in the upper Elbe basin associated with herpesviral hematopoietic necrosis(CyHV-2)[J].Diseases of Aquatic Organisms,2012,102(2):87-95.
[20]Fichi G,Cardeti G,Cocumelli C,et al.Detection of cyprinid herpesvirus 2 in association with an Aeromonas sobria infection of Carassius auratus(L.),in Italy[J].Journal of Fish Diseases,2013,36(10):823-830.
[21]Boitard P-M,Baud M,Labrut S,et al.First detection of Cyprinid Herpesvirus 2(CyHV-2)in goldfish(Carassius auratus)in France[J].Journal of Fish Diseases,2016,39(6):673-680.
[22]Radosavljevic V,Adamek M,Milicevic V,et al.Occurrence of two novel viral pathogens(CEV and CyHV-2)affecting Serbian cyprinid aquaculture and ichthyofauna[J].Journal of Fish Diseases,2018,41(5):851-854.
[23]李莉娟,罗杨志,刘学芹,等.金鱼鲤疱疹病毒Ⅱ型的分子诊断[J].华中农业大学学报,2013,32(1):92-96.
[24]徐进,曾令兵,杨德国,等.鲤疱疹病毒2型武汉株的分离与鉴定[J].中国水产科学,2013,20(6):1303-1309.
[25]罗杨志,李莉娟,顾泽茂,等.鲤疱疹病毒Ⅱ型的分子特征及流行病学研究[C].中国水产学会鱼病专业委员会学术研讨会论文摘要汇编,2013.
[26]王璐.江苏地区鲫出血病病原的分离、鉴定及检测方法研究[D].南京:南京农业大学,2012.
[27]Ito T,Maeno Y.Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2(CyHV-2)[J].Veterinary Microbiology,2014,169(3-4):128-134.
[28]Wang L,He J,Liang L,et al.Mass mortality caused by Cyprinid Herpesvirus 2(Cy HV-2)in Prussian carp(Carassius gibelio)in China[J].Bull Eur Ass Fish Pathol,2012,32(5):163-173.
[29]吴霆,丁正峰,王文,等.异育银鲫鳃出血流行病学调查和研究[J].水产科学,2014,33(5):283-287.
[30]Goodwin A E,Sadler J,Merry G E,et al.Herpesviral haematopoietic necrosis virus(CyHV-2)infection:case studies from commercial goldfish farms[J].Journal of Fish Diseases,2009,32(3):271-278.
[31]Ding Z F,Xia S Y,Zhao Z M,et al.Histopathological characterization and fluorescence in situ hybridization of cyprinid herpesvirus 2 in cultured Prussian carp,Carassius auratus gibelio in China[J].Journal of Virological Methods,2014,206(7):76-83.
[32]Minamoto T,Pu X,Xie J,et al.Monitoring fish pathogenic viruses in natural lakes in Yunnan,China[J].Limnology,2015,16(1):69-77.
[33]Lovy J,Friend S E.Cyprinid herpesvirus-2 causing mass mortality in goldfish:applying electron microscopy to histological samples for diagnostic virology[J].Diseases of Aquatic Organisms,2014,108(1):1-9.
[34]林秀秀,叶元土,吴萍,等.鲤疱疹2型病毒(CyHV-2)感染对异育银鲫(Carassius auratus gibelio)组织器官的损伤作用[J].基因组学与应用生物学,2016,35(3):587-594.
[35]方进,邓院生,王俊,等.急性病毒性鲫鳃出血病的病理变化[J].中国水产科学,2016,23(2):336-343.
[36]Ito T,Kurita J,Ozaki A,et al.Growth of cyprinid herpesvirus 2(CyHV-2)in cell culture and experimental infection of goldfish Carassius auratus[J].Diseases of Aquatic Organisms,2013,105(3):193-202.
[37]Ma J,Jiang N,La Patra S E,et al.Establishment of a novel and highly permissive cell line for the efficient replication of cyprinid herpesvirus 2(CyHV-2)[J].Veterinary Microbiology,2015,177(3-4):315-325.
[38]罗丹,梁利国,谢骏,等.2型鲤疱疹病毒双重PCR快速检测方法的建立及应用[J].中国预防兽医学报,2014,36(5):379-382.
[39]Goodwin A E,Merry G E,Sadler J.Detection of the herpesviral hematopoietic necrosis disease agent(cyprinid herpesvirus 2)in moribund and healthy goldfish:validation of a quantitative PCR diagnostic method[J].Dis Aquat Org,2006,69(2-3):137-143.
[40]徐晔,段宏安,周毅,等.鲤疱疹病毒2型TaqMan荧光定量PCR检测方法的建立[J].中国预防兽医学报,2014,36(12):952-956.
[41]于力,王津津,史秀杰等.鲤科疱疹病毒2型Taqman实时荧光PCR方法的建立[J].中国动物检疫,2015,32(10):80-84.
[42]He J,Shi X,Yu L,et al.Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of cyprinid herpesvirus 2[J].Journal of Virological Method,2013,194(1-2):206-210.
[43]Liang L G,Xie J,Luo D.Development of a rapid cyprinid herpesvirus 2 detection method by loop-mediated isothermal amplification[J].Lett Appl Microbiol,2014,59(4):432-437.
[44]Zhu M,Liu B,Cao G,et al.Identification and rapid diagnosis of the pathogen responsible for haemorrhagic disease of the gill of allogynogenetic crucian carp[J].Journal of Virological Methods,2015,219:67-74.
[45]Zhang H,Zeng L,Fan Y,et al.A loop-mediated isothermal amplification assay for rapid detection of cyprinid herpesvirus 2 in gibel carp(Carassius auratus gibelio)[J].Scientific World Journal,2014,2014:doi:10.1155/2014/716413.
[46]郝中香,林华,佘容,等.鲤科疱疹病毒2型微滴式数字PCR快速检测方法的建立[J].中国兽医科学,2016,46(2):167-173.
[47]孔善云,姜有声,吕利群,等.一种基于II型鲤疱疹病毒衣壳蛋白72的免疫学检测方法[J].中国水产科学,2016,23(2):328-335.
[48]Kong S K,Jiang Y S,Lu L Q,et al.Detection methods of Cyprinid herpesvirus 2 infection in silver crucian carp(Carassius auratus gibelio)via a pORF72 monoclonal antibody[J].Journal of Fish Diseases,2017,40(12):1791-1798.
[49]陈超然,陈萱,陈昌福,等.酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用[J].华中农业大学学报,2003,22(4):380-384.
[50]陈昌福,方苹,胡明,等.酵母多糖对受免异育银鲫免疫保护力的增强作用[J].当代水产,2015(7):82-84.
[51]许淑英,李焕林,邓国成,等.草鱼出血病细胞培养弱毒疫苗的制备及其免疫效果[J].水产学报,1994,18(2):110-117.
[52]孙建和,陈怀青,路承平.嗜水气单胞菌疫苗的研制[J].畜牧与兽医,1995,27(5):202-205.
[53]肖慧,李军,王祥红,等.鲈鱼鳗弧菌病疫苗的制备及免疫防治效果[J].青岛海洋大学学报,2003,33(2):226-232.
[54]曹剑香,简纪常,吴灶和.溶藻弧菌疫苗对凡纳滨对虾免疫功能的影响[J].湛江海洋大学学报,2004,24(6):11-17.
[55]余晓丽,陈明,李莉萍.罗非鱼海豚链球菌疫苗及其免疫效果的研究[J].淡水渔业,2008,38(6):31-37.
[56]秦启伟,潘金培.创伤弧菌疫苗对青石斑鱼的免疫学效应和安全性[J].热带海洋,1996,15(2):7-13.
[57]Ito T,Ototake.Vaccination against cyprinid herpesvirus 2(CyHV-2)infection in goldfish Carassius auratus[J].Bull Eur Ass Fish Pathol,2013,33(5):158-164.
[58]Ito T,Maeno Y.Effect of booster shot and investigation of vaccination efficacy period against herpesviral haematopoietic necrosis(HVHN)in goldfish Carassius auratus[J].Veterinary Microbiology,2015,175(1):139-144.
[59]Zhang L,Ma J,Fan Y,et al.Immune response and protection in gibel carp,Carassius gibelio,after vaccination withβ-propiolactone inactivated cyprinid herpesvirus 2[J].Fish&Shellfish Immunology,2016,49(1):344-350.
[60]廖红,林华,郝中香,等.鲤疱疹病毒2型ORF5截短基因的克隆表达及免疫原性研究[J].中国兽医科学,2016(11):1394-1400.
[2]Waltzek T B,Kelley G O,Stone D M,et al.Koi herpesvirus representsathirdcyprinidherpesvirus(CyHV-3)inthefamily Herpesviridae[J].J Gen Virol,2005,86(6):1659-1667.
[3]Davision A J,Eberle R,Ehlers B,et al.The order Herpesvirales[J].Arch Virol,2009,154(1):171-177.
[4]Hanson L,Dishon A,Kotler M.Herpesviruses that infect fish viruses[J].Viruses,2011,3(11):2160-2191.
[5]Doszpoly A,Benko M,Csaba G,et al.Introduction of the Family Alloherpesviridae:the first molecular detection of herpesviruses of cyprinid fish in Hungary[J].Magyar Allatorvosok Lapja,2011,133(3):174-181.
[6]Xu J,Zeng L B,Zhang H,et al.Cyprinid herpesvirus 2 infection emerged in cultured gibel carp,Carassius gibelio in China[J].Veterinary Microbiology,2013,166(1-2):138-144.
[7]Groff J M,La Patra S E,Munn R J,et al.A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology[J].J Vet Diagn Invest,1998,10(4):375-378.
[8]Li L J,Luo Y Z,Gao Z X,et al.Molecular characterisation and prevalence of a new genotype of Cyprinid herpesvirus 2in mainland China[J].Can.J.Microbiol,2015,61(6):381-387.
[9]Davlson A J,Kurobe T,Gatherer D,et al.Comparative genomics of carp herpesviruses[J].Journal of Virology,2013,87(5):2908-2922.
[10]Zhou Y,Jiang N,Ma J,et al.Protective immunity in gibel carp,Carassius gibelio of the truncated proteins of cyprinid herpesvirus 2 expressed in Pichia pastoris[J].Fish&Shellfish Immunology,2015,47(2):1024-1031.
[11]Du M,Chen M L,Shen H F,et al.CyHV-2 ORF104 activates the p38 MAPK pathway[J].Fish&Shellfish Immunology,2015,46(2):268-273.
[12]Chang P H,Lee S H,Chiang H C,et al.Epizootic of herpes-like virus infection in goldfish,Carassius auratus in Taiwan[J].Fish Pathology,1999,34(4):209-210.
[13]Groff J M,La Patra S E,Munn R J,et al.A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology[J].J Vet Diagn Invest,1998,10(4):375-378[g1].
[14]Stephens F J,Raidal S R,Jones B.Haematopoietic necrosis in a goldfish(Carassius auratus)associated with an agent morphologically similar to herpesvirus[J].Australian Veterinary Journal,2004,82(3):167-169.
[15]Hine P M,Tham K M,Morrison R,et al.Cyprinid herpersvirus 2 in New Zealand goldfish[J].Surveillance,2006,33(4):3-5.
[16]Jeffery K R,Bateman K,Bayley A,et al.Isolation of a cyprinid herpesvirus 2 from goldfish Carassius auratus(L.),in the UK[J].J Fish Dis,2007,30(11):649-656.
[17]Doszpoly A,Benko M,Csaba G,et al.Introduction of the family Alloherpesviridae:the first molecular detection of herpesviruses of cyprinid fish in Hungary[J].Magyar Allatorvosok Lapja,2011,133(3):174-181.
[18]Wu T,Ding Z,Ren M,et al.The histo-and ultra-pathological studies on a fatal disease of Prussian carp(Carassius auratus)in mainland China associated with cyprinid herpesvirus 2(CyHV-2)[J].Aquaculture,2013,412-413(6):8-13.
[19]Daněk T,Kalous L,Vesel T,et al.Massive mortality of Prussian carp Carassius gibelio in the upper Elbe basin associated with herpesviral hematopoietic necrosis(CyHV-2)[J].Diseases of Aquatic Organisms,2012,102(2):87-95.
[20]Fichi G,Cardeti G,Cocumelli C,et al.Detection of cyprinid herpesvirus 2 in association with an Aeromonas sobria infection of Carassius auratus(L.),in Italy[J].Journal of Fish Diseases,2013,36(10):823-830.
[21]Boitard P-M,Baud M,Labrut S,et al.First detection of Cyprinid Herpesvirus 2(CyHV-2)in goldfish(Carassius auratus)in France[J].Journal of Fish Diseases,2016,39(6):673-680.
[22]Radosavljevic V,Adamek M,Milicevic V,et al.Occurrence of two novel viral pathogens(CEV and CyHV-2)affecting Serbian cyprinid aquaculture and ichthyofauna[J].Journal of Fish Diseases,2018,41(5):851-854.
[23]李莉娟,罗杨志,刘学芹,等.金鱼鲤疱疹病毒Ⅱ型的分子诊断[J].华中农业大学学报,2013,32(1):92-96.
[24]徐进,曾令兵,杨德国,等.鲤疱疹病毒2型武汉株的分离与鉴定[J].中国水产科学,2013,20(6):1303-1309.
[25]罗杨志,李莉娟,顾泽茂,等.鲤疱疹病毒Ⅱ型的分子特征及流行病学研究[C].中国水产学会鱼病专业委员会学术研讨会论文摘要汇编,2013.
[26]王璐.江苏地区鲫出血病病原的分离、鉴定及检测方法研究[D].南京:南京农业大学,2012.
[27]Ito T,Maeno Y.Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2(CyHV-2)[J].Veterinary Microbiology,2014,169(3-4):128-134.
[28]Wang L,He J,Liang L,et al.Mass mortality caused by Cyprinid Herpesvirus 2(Cy HV-2)in Prussian carp(Carassius gibelio)in China[J].Bull Eur Ass Fish Pathol,2012,32(5):163-173.
[29]吴霆,丁正峰,王文,等.异育银鲫鳃出血流行病学调查和研究[J].水产科学,2014,33(5):283-287.
[30]Goodwin A E,Sadler J,Merry G E,et al.Herpesviral haematopoietic necrosis virus(CyHV-2)infection:case studies from commercial goldfish farms[J].Journal of Fish Diseases,2009,32(3):271-278.
[31]Ding Z F,Xia S Y,Zhao Z M,et al.Histopathological characterization and fluorescence in situ hybridization of cyprinid herpesvirus 2 in cultured Prussian carp,Carassius auratus gibelio in China[J].Journal of Virological Methods,2014,206(7):76-83.
[32]Minamoto T,Pu X,Xie J,et al.Monitoring fish pathogenic viruses in natural lakes in Yunnan,China[J].Limnology,2015,16(1):69-77.
[33]Lovy J,Friend S E.Cyprinid herpesvirus-2 causing mass mortality in goldfish:applying electron microscopy to histological samples for diagnostic virology[J].Diseases of Aquatic Organisms,2014,108(1):1-9.
[34]林秀秀,叶元土,吴萍,等.鲤疱疹2型病毒(CyHV-2)感染对异育银鲫(Carassius auratus gibelio)组织器官的损伤作用[J].基因组学与应用生物学,2016,35(3):587-594.
[35]方进,邓院生,王俊,等.急性病毒性鲫鳃出血病的病理变化[J].中国水产科学,2016,23(2):336-343.
[36]Ito T,Kurita J,Ozaki A,et al.Growth of cyprinid herpesvirus 2(CyHV-2)in cell culture and experimental infection of goldfish Carassius auratus[J].Diseases of Aquatic Organisms,2013,105(3):193-202.
[37]Ma J,Jiang N,La Patra S E,et al.Establishment of a novel and highly permissive cell line for the efficient replication of cyprinid herpesvirus 2(CyHV-2)[J].Veterinary Microbiology,2015,177(3-4):315-325.
[38]罗丹,梁利国,谢骏,等.2型鲤疱疹病毒双重PCR快速检测方法的建立及应用[J].中国预防兽医学报,2014,36(5):379-382.
[39]Goodwin A E,Merry G E,Sadler J.Detection of the herpesviral hematopoietic necrosis disease agent(cyprinid herpesvirus 2)in moribund and healthy goldfish:validation of a quantitative PCR diagnostic method[J].Dis Aquat Org,2006,69(2-3):137-143.
[40]徐晔,段宏安,周毅,等.鲤疱疹病毒2型TaqMan荧光定量PCR检测方法的建立[J].中国预防兽医学报,2014,36(12):952-956.
[41]于力,王津津,史秀杰等.鲤科疱疹病毒2型Taqman实时荧光PCR方法的建立[J].中国动物检疫,2015,32(10):80-84.
[42]He J,Shi X,Yu L,et al.Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of cyprinid herpesvirus 2[J].Journal of Virological Method,2013,194(1-2):206-210.
[43]Liang L G,Xie J,Luo D.Development of a rapid cyprinid herpesvirus 2 detection method by loop-mediated isothermal amplification[J].Lett Appl Microbiol,2014,59(4):432-437.
[44]Zhu M,Liu B,Cao G,et al.Identification and rapid diagnosis of the pathogen responsible for haemorrhagic disease of the gill of allogynogenetic crucian carp[J].Journal of Virological Methods,2015,219:67-74.
[45]Zhang H,Zeng L,Fan Y,et al.A loop-mediated isothermal amplification assay for rapid detection of cyprinid herpesvirus 2 in gibel carp(Carassius auratus gibelio)[J].Scientific World Journal,2014,2014:doi:10.1155/2014/716413.
[46]郝中香,林华,佘容,等.鲤科疱疹病毒2型微滴式数字PCR快速检测方法的建立[J].中国兽医科学,2016,46(2):167-173.
[47]孔善云,姜有声,吕利群,等.一种基于II型鲤疱疹病毒衣壳蛋白72的免疫学检测方法[J].中国水产科学,2016,23(2):328-335.
[48]Kong S K,Jiang Y S,Lu L Q,et al.Detection methods of Cyprinid herpesvirus 2 infection in silver crucian carp(Carassius auratus gibelio)via a pORF72 monoclonal antibody[J].Journal of Fish Diseases,2017,40(12):1791-1798.
[49]陈超然,陈萱,陈昌福,等.酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用[J].华中农业大学学报,2003,22(4):380-384.
[50]陈昌福,方苹,胡明,等.酵母多糖对受免异育银鲫免疫保护力的增强作用[J].当代水产,2015(7):82-84.
[51]许淑英,李焕林,邓国成,等.草鱼出血病细胞培养弱毒疫苗的制备及其免疫效果[J].水产学报,1994,18(2):110-117.
[52]孙建和,陈怀青,路承平.嗜水气单胞菌疫苗的研制[J].畜牧与兽医,1995,27(5):202-205.
[53]肖慧,李军,王祥红,等.鲈鱼鳗弧菌病疫苗的制备及免疫防治效果[J].青岛海洋大学学报,2003,33(2):226-232.
[54]曹剑香,简纪常,吴灶和.溶藻弧菌疫苗对凡纳滨对虾免疫功能的影响[J].湛江海洋大学学报,2004,24(6):11-17.
[55]余晓丽,陈明,李莉萍.罗非鱼海豚链球菌疫苗及其免疫效果的研究[J].淡水渔业,2008,38(6):31-37.
[56]秦启伟,潘金培.创伤弧菌疫苗对青石斑鱼的免疫学效应和安全性[J].热带海洋,1996,15(2):7-13.
[57]Ito T,Ototake.Vaccination against cyprinid herpesvirus 2(CyHV-2)infection in goldfish Carassius auratus[J].Bull Eur Ass Fish Pathol,2013,33(5):158-164.
[58]Ito T,Maeno Y.Effect of booster shot and investigation of vaccination efficacy period against herpesviral haematopoietic necrosis(HVHN)in goldfish Carassius auratus[J].Veterinary Microbiology,2015,175(1):139-144.
[59]Zhang L,Ma J,Fan Y,et al.Immune response and protection in gibel carp,Carassius gibelio,after vaccination withβ-propiolactone inactivated cyprinid herpesvirus 2[J].Fish&Shellfish Immunology,2016,49(1):344-350.
[60]廖红,林华,郝中香,等.鲤疱疹病毒2型ORF5截短基因的克隆表达及免疫原性研究[J].中国兽医科学,2016(11):1394-1400.