急性低温胁迫对条纹锯鮨血清生化指标的影响
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摘要
[目的]分析不同急性低温胁迫强度、胁迫持续时间下条纹锯鮨血清生化指标的变化,为其健康养殖和耐寒机制研究提供理论依据。[方法]以条纹锯鮨为研究对象,分别从暂养水温13℃急性胁迫到3、5、7℃,分析3个急性低温胁迫强度、不同胁迫时间(0、2、6、12、24、48 h)对条纹锯鮨血清生化指标的影响。[结果]Ⅰ、Ⅱ组谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)活性呈先升后降的趋势,各组碱性磷酸酶(ALP)活性均显著高于胁迫前(P<0.05);Ⅰ、Ⅱ组血清总胆固醇(TC)和甘油三酯(TG)先降后升至胁迫前水平,12 h后Ⅲ组总蛋白(TP)、TG、TC含量均显著低于胁迫前水平(P<0.05),Ⅰ、Ⅱ组葡萄糖(GLU)含量48 h时显著上升(P<0.05);Ⅰ、Ⅱ组48 h时血清离子与胁迫前无显著差异(P>0.05),Ⅲ组K~+和Ca~(2+)含量显著下降(P<0.05),Na~+和Cl~-含量显著上升(P<0.05)。[结论]在条纹锯鮨养殖生产和低温驯养过程中,应尽量降低胁迫强度和缩短胁迫时间。
[Objective] To analyze the changes of serum biochemical parameters in Centropristis striata under different acute low temperature stress and the duration of the stress and provide theoretical basis for healthy breeding and cold-resistant mechanism of C. striata. [Method] Using C. striata as research subject, the effects of three acute cooling stress intensities( from the temporary culture temperature 13 ℃ to 3, 5, 7 ℃ in group Ⅰ,Ⅱ and Ⅲ respectively) and different duration(0, 2, 6, 12, 24 and 48 h) on the serum biochemical parameters of C. striata were analyzed. [Result] The activities of serum ALT, AST, LDH and CK-MB in Ⅰ,Ⅱ groups showed the change trend of first increasing and then decreasing.ALP activity in each group was significantly higher than that before the stress(P<0.05). TC and TG in the serum in Ⅰ,Ⅱ groups first decreased and then increased up to the level before the stress, and TP, TG, TC contents in Ⅲ group after 12 h were significantly lower than those before the stress(P<0.05). The glucose(GLU) content in Ⅱ group at 48 h significantly increased(P<0.05), serum ion in Ⅰ and Ⅱ groups at 48 h had no significant difference with that before the stress(P>0.05). The contents of K~+, Ca~(2+) in group Ⅲ decreased significantly(P<0.05), Na~+ and Cl~- contents significantly increased(P<0.05).[Conclusion] The stress intensity and stress time should be reduced as possible, in the process of breeding production and low temperature domestication.
[Objective] To analyze the changes of serum biochemical parameters in Centropristis striata under different acute low temperature stress and the duration of the stress and provide theoretical basis for healthy breeding and cold-resistant mechanism of C. striata. [Method] Using C. striata as research subject, the effects of three acute cooling stress intensities( from the temporary culture temperature 13 ℃ to 3, 5, 7 ℃ in group Ⅰ,Ⅱ and Ⅲ respectively) and different duration(0, 2, 6, 12, 24 and 48 h) on the serum biochemical parameters of C. striata were analyzed. [Result] The activities of serum ALT, AST, LDH and CK-MB in Ⅰ,Ⅱ groups showed the change trend of first increasing and then decreasing.ALP activity in each group was significantly higher than that before the stress(P<0.05). TC and TG in the serum in Ⅰ,Ⅱ groups first decreased and then increased up to the level before the stress, and TP, TG, TC contents in Ⅲ group after 12 h were significantly lower than those before the stress(P<0.05). The glucose(GLU) content in Ⅱ group at 48 h significantly increased(P<0.05), serum ion in Ⅰ and Ⅱ groups at 48 h had no significant difference with that before the stress(P>0.05). The contents of K~+, Ca~(2+) in group Ⅲ decreased significantly(P<0.05), Na~+ and Cl~- contents significantly increased(P<0.05).[Conclusion] The stress intensity and stress time should be reduced as possible, in the process of breeding production and low temperature domestication.
引文
[1] 雷霁霖,卢继武.美洲黑石斑鱼的品种优势和养殖前景[J].海洋水产研究,2007,28(5):110-115.
[2] 刘瑞棠.美洲黑石斑鱼海水网箱养殖技术[J].海洋与渔业,2012(4):59.
[3] 郑国洪.美洲黑石斑与对虾、花蛤混养试验[J].海洋与渔业,2012(1):62-63.
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[5] 毕浩,侯文杰.陆基循环水养殖美洲黑石斑技术[J].水产科技情报,2017,44(3):128-130.
[6] 潘元潮,杨心德,陆波.美洲黑石斑大规格鱼种培育技术[J].水产养殖,2013(2):31-32.
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[9] 韩龙江,刘清华,官曙光,等.条纹锯鮨精液超低温冷冻保存研究[J].水产学报,2014,38(10):1714-1721.
[10] 陈超,贾瑞锦,李炎璐,等.条纹锯鮨精子超微结构及其入卵过程的电镜观察[J].渔业科学进展,2014,35(5):97-103.
[11] 林星.条纹锯鮨幼鱼配合饲料适宜的蛋白质、脂类含量及能蛋比[J].福建农林大学学报(自然科学版),2011,40(4):401-406.
[12] 林星.条纹锯鮨肌肉营养成分分析与品质评价[J].养殖与饲料,2009(9):43-46.
[13] 党冉,竺俊全,邱新志.美洲黑石斑鱼含肉率及肌肉营养成分分析[J].海洋学研究,2010,28(2):60-66.
[14] 郑磊,马振宁,吴斌,等.美洲黑石斑鱼血清IgM纯化及其兔抗血清部分特性[J].上海海洋大学学报,2011,20(4):494-498.
[15] 陈超,施兆鸿,薛宝贵,等.低温胁迫对七带石斑鱼幼鱼血清生化指标的影响[J].水产学报,2012,36(8):1249-1255.
[16] 朱文彬,刘浩亮,陈作志,等.低温胁迫对马来西亚红罗非鱼血清生化指标的影响[J].水产学杂志,2013,26(5):16-20.
[17] 冀德伟,李明云,王天柱,等.不同低温胁迫时间对大黄鱼血清生化指标的影响[J].水产科学,2009,28(1):1-4.
[18] 宁军号,秦宇博,胡伦超,等.水温骤降和缓降胁迫对褐篮子鱼血液生理生化指标的影响[J].大连海洋大学学报,2017,32(3):294-301.
[19] 何福林,向建国,李常健,等.水温对虹鳟血液学指标影响的初步研究[J].水生生物学报,2007,31(3):363-369.
[20] 臧坤,徐永江,柳学周,等.两种养殖条件下牙鲆的血液生理生化指标比较分析[J].渔业科学进展,2013,34(5):36-42.
[21] PANSERAT S,PERRIN A,KAUSHIK S.High dietary lipids induce liver glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss)[J].The journal of nutrition,2002,132(2):137-141.
[22] 邵同先,张苏亚,康健,等.低温环境对家兔血清蛋白、血糖和钙含量的影响[J].环境与健康杂志,2002,19(5):379-380.
[23] 刘波,王美垚,谢骏,等.低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响[J].生态学报,2011,31(17):4866-4873.
[24] 常玉梅,匡友谊,曹鼎臣,等.低温胁迫对鲤血液学和血清生化指标的影响[J].水产学报,2006,30(5):701-706.
[25] 谢明媚.急性温度胁迫对银鲳幼鱼各项生化指标的影响[D].上海:上海海洋大学,2016.
[26] 尾崎久雄.鱼类血液与循环生理[M].上海:上海科学技术出版社,1982:151-154.
[27] SANO T.Haematological studies of the culture fishes in Japan[J].Journal of Tokyo University of Fisheries,1962,48:105-109.
[28] HOLMES W N,DONALDSON E M.The body compartments and the distribution of electrolytes [M ]//HOAR W S,RANDALL D J.Fish physiology.New York:Academic Press,1969:1-89.
[29] LEBRETON G T O,BEAMISH F W H.The influence of salinity on ionic concentrations and osmolarity of blood serum in lake sturgeon,Acipenser fulvescens[J].Environmental biology of fishes,1998,52:477-482.
[2] 刘瑞棠.美洲黑石斑鱼海水网箱养殖技术[J].海洋与渔业,2012(4):59.
[3] 郑国洪.美洲黑石斑与对虾、花蛤混养试验[J].海洋与渔业,2012(1):62-63.
[4] 谢书秋.条纹锯鮨海水网箱养殖技术[J].渔业信息与战略,2014,29(4):285-288.
[5] 毕浩,侯文杰.陆基循环水养殖美洲黑石斑技术[J].水产科技情报,2017,44(3):128-130.
[6] 潘元潮,杨心德,陆波.美洲黑石斑大规格鱼种培育技术[J].水产养殖,2013(2):31-32.
[7] 张廷廷,陈超,邵彦翔,等.美洲黑石斑鱼(Centropristis striata)消化系统胚后发育的组织学观察[J].渔业科学进展,2017,38(3):78-85.
[8] 贾瑞锦,王鲁,赵从明,等.条纹锯鱼旨胚胎发育及卵黄囊仔鱼形态变化的观察[J].渔业科学进展,2012,33(4):11-17.
[9] 韩龙江,刘清华,官曙光,等.条纹锯鮨精液超低温冷冻保存研究[J].水产学报,2014,38(10):1714-1721.
[10] 陈超,贾瑞锦,李炎璐,等.条纹锯鮨精子超微结构及其入卵过程的电镜观察[J].渔业科学进展,2014,35(5):97-103.
[11] 林星.条纹锯鮨幼鱼配合饲料适宜的蛋白质、脂类含量及能蛋比[J].福建农林大学学报(自然科学版),2011,40(4):401-406.
[12] 林星.条纹锯鮨肌肉营养成分分析与品质评价[J].养殖与饲料,2009(9):43-46.
[13] 党冉,竺俊全,邱新志.美洲黑石斑鱼含肉率及肌肉营养成分分析[J].海洋学研究,2010,28(2):60-66.
[14] 郑磊,马振宁,吴斌,等.美洲黑石斑鱼血清IgM纯化及其兔抗血清部分特性[J].上海海洋大学学报,2011,20(4):494-498.
[15] 陈超,施兆鸿,薛宝贵,等.低温胁迫对七带石斑鱼幼鱼血清生化指标的影响[J].水产学报,2012,36(8):1249-1255.
[16] 朱文彬,刘浩亮,陈作志,等.低温胁迫对马来西亚红罗非鱼血清生化指标的影响[J].水产学杂志,2013,26(5):16-20.
[17] 冀德伟,李明云,王天柱,等.不同低温胁迫时间对大黄鱼血清生化指标的影响[J].水产科学,2009,28(1):1-4.
[18] 宁军号,秦宇博,胡伦超,等.水温骤降和缓降胁迫对褐篮子鱼血液生理生化指标的影响[J].大连海洋大学学报,2017,32(3):294-301.
[19] 何福林,向建国,李常健,等.水温对虹鳟血液学指标影响的初步研究[J].水生生物学报,2007,31(3):363-369.
[20] 臧坤,徐永江,柳学周,等.两种养殖条件下牙鲆的血液生理生化指标比较分析[J].渔业科学进展,2013,34(5):36-42.
[21] PANSERAT S,PERRIN A,KAUSHIK S.High dietary lipids induce liver glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss)[J].The journal of nutrition,2002,132(2):137-141.
[22] 邵同先,张苏亚,康健,等.低温环境对家兔血清蛋白、血糖和钙含量的影响[J].环境与健康杂志,2002,19(5):379-380.
[23] 刘波,王美垚,谢骏,等.低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响[J].生态学报,2011,31(17):4866-4873.
[24] 常玉梅,匡友谊,曹鼎臣,等.低温胁迫对鲤血液学和血清生化指标的影响[J].水产学报,2006,30(5):701-706.
[25] 谢明媚.急性温度胁迫对银鲳幼鱼各项生化指标的影响[D].上海:上海海洋大学,2016.
[26] 尾崎久雄.鱼类血液与循环生理[M].上海:上海科学技术出版社,1982:151-154.
[27] SANO T.Haematological studies of the culture fishes in Japan[J].Journal of Tokyo University of Fisheries,1962,48:105-109.
[28] HOLMES W N,DONALDSON E M.The body compartments and the distribution of electrolytes [M ]//HOAR W S,RANDALL D J.Fish physiology.New York:Academic Press,1969:1-89.
[29] LEBRETON G T O,BEAMISH F W H.The influence of salinity on ionic concentrations and osmolarity of blood serum in lake sturgeon,Acipenser fulvescens[J].Environmental biology of fishes,1998,52:477-482.