塑料饲养大蜡螟幼虫肠道可培养细菌多样性
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摘要
【背景】昆虫肠道中存在大量的微生物,是昆虫正常生命活动所必需的。它们能够促进维生素的合成、脂肪和碳水化合物的吸收及利用,同时还可以保护宿主抵御天敌,忍受高温以及促进毒素或异生素的代谢,间接促进资源开发。【目的】研究PE塑料饲喂的大蜡螟幼虫肠道可培养细菌的多样性。【方法】利用16S rRNA基因序列分析技术,结合菌落形态和细胞形态及相关生理生化特征鉴定细菌种类。【结果】从大蜡螟幼虫肠道分离纯化的40株可培养细菌得到16种不同细菌遗传型,分别属于芽孢杆菌科(Bacillaceae)、肠球菌科(Enterococcaceae)、葡萄球菌科(Staphylococcaceae)、莫拉菌科(Moraxellaceae)4个科。其中芽孢杆菌科是肠道可培养细菌的优势细菌种类。结合菌落和细胞形态及生理生化特征,确定肠道可培养细菌为芽孢杆菌属9株、肠球菌属4株、葡萄球菌属2株以及不动杆菌属1株。【结论】通过研究大蜡螟幼虫肠道可培养细菌群落结构组成,可为开展大蜡螟肠道的微生态研究提供相关理论基础。
[Background] There are a large number of microorganisms in the intestinal tract of insects, which are necessary for the normal life activities of insects. They promote the synthesis of vitamins, absorption and utilization of fats, carbohydrates and also protect the host against natural enemies, endure high temperature and promote the metabolism of toxins or xenobiotics, and indirectly promote resource development.[Objective] To study the diversity of culturable bacteria isolated from the whole larvae gut of Galleria mellonella. [Methods] A molecular phylogeny of the 16 S rRNA gene, the morphology of the colonies and the cells as well as related physiological and biochemical characteristics were used to identify the bacterial species. [Results] A total of 40 culturable bacteria from the intestine of G. mellonella were classified into16 unique phylotypes. All sequenced bacteria strains were grouped into four families: Bacillaceae,Enterococcaceae, Staphylococcaceae and Moraxellaceae. Among them, Bacillus spp. is the most dominant species of culturable intestinal bacteria. It was determined that the culturable intestinal bacteria included9 strains of Bacillus, 4 strains of Enterococcus, 2 strains of Staphylococcus, and 1 strain of Acinetobacter.[Conclusion] By studying the composition of bacterial communities that can be cultured from the larvae intestine of G. mellonella, this study provides theoretical basis for the microecology research of the gut of G.mellonella.
[Background] There are a large number of microorganisms in the intestinal tract of insects, which are necessary for the normal life activities of insects. They promote the synthesis of vitamins, absorption and utilization of fats, carbohydrates and also protect the host against natural enemies, endure high temperature and promote the metabolism of toxins or xenobiotics, and indirectly promote resource development.[Objective] To study the diversity of culturable bacteria isolated from the whole larvae gut of Galleria mellonella. [Methods] A molecular phylogeny of the 16 S rRNA gene, the morphology of the colonies and the cells as well as related physiological and biochemical characteristics were used to identify the bacterial species. [Results] A total of 40 culturable bacteria from the intestine of G. mellonella were classified into16 unique phylotypes. All sequenced bacteria strains were grouped into four families: Bacillaceae,Enterococcaceae, Staphylococcaceae and Moraxellaceae. Among them, Bacillus spp. is the most dominant species of culturable intestinal bacteria. It was determined that the culturable intestinal bacteria included9 strains of Bacillus, 4 strains of Enterococcus, 2 strains of Staphylococcus, and 1 strain of Acinetobacter.[Conclusion] By studying the composition of bacterial communities that can be cultured from the larvae intestine of G. mellonella, this study provides theoretical basis for the microecology research of the gut of G.mellonella.
引文
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[22]Feng W.Different comparison and characterization of lipase-producing bacteria in the silkworm intestinal microflora[D].Chongqing:Master’s Thesis of Southwest University,2011(in Chinese)冯伟.家蚕肠道微生态产脂肪细菌的分离鉴定与差异性比较[D].重庆:西南大学硕士学位论文,2011
[23]Sun ZL.Effects on the intestinal microbial communities with virus infection and high temperature treatment in Silkworm,Bombyx mori[D].Suzhou:Master’s Thesis of Soochow University,2016(in Chinese)孙振丽.病毒感染和高温处理对家蚕肠道菌群结构的影响[D].苏州:苏州大学硕士学位论文,2016
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[26]Lin XL.Gut bacterial diversity of the diamondback moth and insecticidal activity to larvae by two cultivable bacteria[D].Xianyang:Doctoral Dissertation of Northwest A&F University,2014(in Chinese)林晓丽.小菜蛾肠道细菌多样性分析及两株细菌的杀虫活性研究[D].咸阳:西北农林科技大学博士学位论文,2014
[27]Xia XF.Organizational diversity and functional characterization of microbiota in the midgut of diamondback moth,Plutella xylostella(L.)[D].Fuzhou:Doctoral Dissertation of Fujian Agriculture and Forestry University,2014(in Chinese)夏晓峰.小菜蛾中肠微生物多样性及其功能研究[D].福州:福建农林大学博士学位论文,2014
[28]Zheng DD.Comparative study on the diversity of bacterial communities in diamondback moth(Plutella xylostella)gut at different developmental stages[D].Fuzhou:Master’s Thesis of Fujian Agriculture and Forestry University,2013(in Chinese)郑丹丹.小菜蛾不同发育阶段肠道细菌多样性分析[D].福州:福建农林大学硕士学位论文,2013
[29]Lan BM.Diversity and function of gut bacterial symbions of Spodoptera litura[D].Fuzhou:Master’s Thesis of Fujian Agriculture and Forestry University,2016(in Chinese)蓝波妙.斜纹夜蛾肠道细菌多样性及其对功能研究[D].福州:福建农林大学硕士学位论文,2016
[30]Zou CR.Analysis of bacterial community and the screening and identification of enzyme-producing bacteria in intestine of Antheraer pernyi[D].Hefei:Anhui Agricultural University,2011(in Chinese)邹昌瑞.柞蚕肠道菌群分析及产酶菌的筛选与鉴定[D].合肥:安徽农业大学,2011
[31]Chen TS.Manufacture and Application of Microbial Culture Medium[M].Beijing:China Agriculture Press,1995:179-184(in Chinese)陈天寿.微生物培养基的制造与应用[M].北京:中国农业出版社,1995:179-184
[32]Zhang H,Jackson TA.Autochthonous bacterial flora indicated by PCR-DGGE of 16S r RNA gene fragments from the alimentary tract of Costelytra zealandica(Coleoptera:Scarabaeidae)[J].Journal of Applied Microbiology,2008,105(5):1277-1285
[33]Nangong ZY,Yang J,Wang QY,et al.Study on histology of the alimentary canal of great wax moth Galleria mellonella larvae[J].Journal of Agricultural University of Hebei,2015(5):63-67(in Chinese)南宫自艳,杨君,王勤英,等.大蜡螟幼虫消化道的组织学研究[J].河北农业大学学报,2015(5):63-67
[34]Calheiros CSC,Pereira SIA,Castro PML.Culturable bacteria associated to the rhizosphere and tissues of Iris pseudacorus plants growing in a treatment wetland for winery wastewater discharge[J].Ecological Engineering,2018,115:67-74
[35]Wang HX,Jin L,Chen QL,et al.Diversity and community structure of intestinal cultivable bacteria in adult worker-bee[J].Microbiology China,2017,44(3):620-630(in Chinese)王洪秀,靳亮,陈庆隆,等.蜜蜂成虫工蜂肠道可培养细菌群落结构分析[J].微生物学通报,2017,44(3):620-630
[36]Thompson JD,Higgins DG,Gibson TJ.CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J].Nucleic Acids Research,1994,22(22):4673-4680
[37]Kumar S,Stecher G,Tamura K.MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Molecular Biology&Evolution,2016,33(7):1870-1874
[38]Pandiarajan J,Krishnan M.Comparative bacterial survey in the gut of lepidopteran insects with different bionetwork[J].Microbiology,2018,87(1):103-115
[39]Demirci M,Sevim E,Demir I,et al.Culturable bacterial microbiota of Plagiodera versicolora(L.)(Coleoptera:Chrysomelidae)and virulence of the isolated strains[J].Folia Microbiologica,2013,58(3):201-210
[40]Hinojosa VS,Asenjo J,Leiva S.Agarolytic culturable bacteria associated with three antarctic subtidal macroalgae[J].World Journal of Microbiology&Biotechnology,2018,34(6):73
[41]Paniagua Voirol LR,Frago E,Kaltenpoth M,et al.Bacterial symbionts in Lepidoptera:Their diversity,transmission,and impact on the host[J].Frontiers in Microbiology,2018,9:556
[42]Gilliam M.Microbes from apiarian sources:Bacillus spp.in frass of the greater wax moth[J].Journal of Invertebrate Pathology,1985,45(2):218-224
[43]Jarosz J.Gut flora of Galleria mellonella suppressing ingested bacteria[J].Journal of Invertebrate Pathology,1979,34(2):192-198
[44]Dillon RJ,Charnley AK.Chemical barriers to gut infection in the desert locust:in vivo production of antimicrobial phenols associated with the bacterium Pantoea agglomerans[J].Journal of Invertebrate Pathology,1995,66(1):72-75
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[2]Smith TL.External morphology of the larva,pupa,and adult of the wax moth,Galleria mellonella L.[J].Journal of the Kansas Entomological Society,1965,38(3):287-310
[3]Reza J,Shila G,Shahram A.Control of the wax moth Galleria mellonella L.(Lepidoptera:Pyralidae)by the male sterile technique(MST)[J].Archives of Biological Sciences,2010,62(2):309-313
[4]Liu QZ,Tian L,Pu HH.Utilization status of the greater wax moth,Galleria mellonella,as an experimental insect[J].Chinese Bulletin of Entomology,2009,46(3):485-489(in Chinese)刘奇志,田里,蒲恒浒.大蜡螟作为试验昆虫资源的利用现状[J].昆虫知识,2009,46(3):485-489
[5]Liu R,Liu QZ.Status Quo and Prospects of research and industrial development on greater wax worm at home and abroad[J].Chinese Agricultural Science Bulletin,2015,31(28):280-284(in Chinese)刘瑞,刘奇志.国内外大蜡螟研究与产业发展现状及展望[J].中国农学通报,2015,31(28):280-284
[6]Senior NJ,Bagnall MC,Champion OL,et al.Galleria mellonella as an infection model for Campylobacter jejuni virulence[J].Journal of Medical Microbiology,2011,60(5):661-669
[7]Mukherjee K,Raju R,Fischer R,et al.Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen Listeria monocytogenes[A]//Advances in Biochemical Engineering/Biotechnology[M].Berlin,Heidelberg:Springer,2013:27-39
[8]McMillan S,Verner-Jeffreys D,Weeks J,et al.Larva of the greater wax moth,Galleria mellonella,is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum[J].BMC Microbiology,2015,15:127
[9]Ibrahim AA,Mohamed HF,El-Naggar SEM,et al.Isolation and selection of entomopathogenic Fungi as biocontrol agent against the greater wax moth,Galleria mellonella L.(Lepidoptera:Pyralidae)[J].Egyptian Journal of Pest Control,2016,26(2):249-253
[10]Bombelli P,Howe CJ,Bertocchini F.Polyethylene bio-degradation by caterpillars of the wax moth Galleria mellonella[J].Current Biology,2017,27(8):R292-R293
[11]Yang J,Yang Y,Wu WM,et al.Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms[J].Environmental Science&Technology,2014,48(23):13776-13784
[12]Sommer F,B?ckhed F.The gut microbiota--masters of host development and physiology[J].Nature Reviews Microbiology,2013,11(4):227-238
[13]Ventura M,Turroni F,Canchaya C,et al.Microbial diversity in the human intestine and novel insights from metagenomics[J].Frontiers in Bioscience,2009,14(9):3214-3221
[14]Kroiss J,Kaltenpoth M,Schneider B,et al.Symbiotic streptomycetes provide antibiotic combination prophylaxis for wasp offspring[J].Nature Chemical Biology,2010,6(4):261-263
[15]Dunbar HE,Wilson ACC,Ferguson NR,et al.Aphid thermal tolerance is governed by a Point mutation in bacterial symbionts[J].PLoS Biology,2007,5(5):e96
[16]Douglas AE.The microbial dimension in insect nutritional ecology[J].Functional Ecology,2009,23(1):38-47
[17]Tsuchida T,Koga R,Matsumoto S,et al.Interspecific symbiont transfection confers a novel ecological trait to the recipient insect[J].Biology Letters,2011,7(2):245-248
[18]Dillon RJ,Dillon VM.The gut bacteria of insects:nonpathogenic interactions[J].Annual Review of Entomology,2004,49(1):71-92
[19]Dillon RJ,Vennard CT,Buckling A,et al.Diversity of locust gut bacteria protects against pathogen invasion[J].Ecology Letters,2005,8(12):1291-1298
[20]Dillon RJ,Charnley K.Mutualism between the desert locust Schistocerca gregaria and its gut microbiota[J].Research in Microbiology,2002,153(8):503-509
[21]Yang H.Diversity of gut bacteria in larval of four lepidopteran insect species[D].Nanjing:Master’s Thesis of Nanjing Agricultural University,2012(in Chinese)杨焊.四种鳞翅目害虫肠道细菌多样性分析[D].南京:南京农业大学硕士学位论文,2012
[22]Feng W.Different comparison and characterization of lipase-producing bacteria in the silkworm intestinal microflora[D].Chongqing:Master’s Thesis of Southwest University,2011(in Chinese)冯伟.家蚕肠道微生态产脂肪细菌的分离鉴定与差异性比较[D].重庆:西南大学硕士学位论文,2011
[23]Sun ZL.Effects on the intestinal microbial communities with virus infection and high temperature treatment in Silkworm,Bombyx mori[D].Suzhou:Master’s Thesis of Soochow University,2016(in Chinese)孙振丽.病毒感染和高温处理对家蚕肠道菌群结构的影响[D].苏州:苏州大学硕士学位论文,2016
[24]Xiang YQ.Comparative study of the composition and type of dominant intestinal microflora in Silkworm reared with different forages[D].Chongqing:Master’s Thesis of Southwest University,2010(in Chinese)向芸庆.不同饲料饲养家蚕其肠道微生态优势菌群的类型组成及差异性研究[D].重庆:西南大学硕士学位论文,2010
[25]He C.Molecular identification of the intestinal bacterial community of Mythimna separate(Walker)larvae[D].Xianyang:Master’s Thesis of Northwest A&F University,2012(in Chinese)何彩.粘虫幼虫肠道细菌群的分子鉴定[D].咸阳:西北农林科技大学硕士学位论文,2012
[26]Lin XL.Gut bacterial diversity of the diamondback moth and insecticidal activity to larvae by two cultivable bacteria[D].Xianyang:Doctoral Dissertation of Northwest A&F University,2014(in Chinese)林晓丽.小菜蛾肠道细菌多样性分析及两株细菌的杀虫活性研究[D].咸阳:西北农林科技大学博士学位论文,2014
[27]Xia XF.Organizational diversity and functional characterization of microbiota in the midgut of diamondback moth,Plutella xylostella(L.)[D].Fuzhou:Doctoral Dissertation of Fujian Agriculture and Forestry University,2014(in Chinese)夏晓峰.小菜蛾中肠微生物多样性及其功能研究[D].福州:福建农林大学博士学位论文,2014
[28]Zheng DD.Comparative study on the diversity of bacterial communities in diamondback moth(Plutella xylostella)gut at different developmental stages[D].Fuzhou:Master’s Thesis of Fujian Agriculture and Forestry University,2013(in Chinese)郑丹丹.小菜蛾不同发育阶段肠道细菌多样性分析[D].福州:福建农林大学硕士学位论文,2013
[29]Lan BM.Diversity and function of gut bacterial symbions of Spodoptera litura[D].Fuzhou:Master’s Thesis of Fujian Agriculture and Forestry University,2016(in Chinese)蓝波妙.斜纹夜蛾肠道细菌多样性及其对功能研究[D].福州:福建农林大学硕士学位论文,2016
[30]Zou CR.Analysis of bacterial community and the screening and identification of enzyme-producing bacteria in intestine of Antheraer pernyi[D].Hefei:Anhui Agricultural University,2011(in Chinese)邹昌瑞.柞蚕肠道菌群分析及产酶菌的筛选与鉴定[D].合肥:安徽农业大学,2011
[31]Chen TS.Manufacture and Application of Microbial Culture Medium[M].Beijing:China Agriculture Press,1995:179-184(in Chinese)陈天寿.微生物培养基的制造与应用[M].北京:中国农业出版社,1995:179-184
[32]Zhang H,Jackson TA.Autochthonous bacterial flora indicated by PCR-DGGE of 16S r RNA gene fragments from the alimentary tract of Costelytra zealandica(Coleoptera:Scarabaeidae)[J].Journal of Applied Microbiology,2008,105(5):1277-1285
[33]Nangong ZY,Yang J,Wang QY,et al.Study on histology of the alimentary canal of great wax moth Galleria mellonella larvae[J].Journal of Agricultural University of Hebei,2015(5):63-67(in Chinese)南宫自艳,杨君,王勤英,等.大蜡螟幼虫消化道的组织学研究[J].河北农业大学学报,2015(5):63-67
[34]Calheiros CSC,Pereira SIA,Castro PML.Culturable bacteria associated to the rhizosphere and tissues of Iris pseudacorus plants growing in a treatment wetland for winery wastewater discharge[J].Ecological Engineering,2018,115:67-74
[35]Wang HX,Jin L,Chen QL,et al.Diversity and community structure of intestinal cultivable bacteria in adult worker-bee[J].Microbiology China,2017,44(3):620-630(in Chinese)王洪秀,靳亮,陈庆隆,等.蜜蜂成虫工蜂肠道可培养细菌群落结构分析[J].微生物学通报,2017,44(3):620-630
[36]Thompson JD,Higgins DG,Gibson TJ.CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J].Nucleic Acids Research,1994,22(22):4673-4680
[37]Kumar S,Stecher G,Tamura K.MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Molecular Biology&Evolution,2016,33(7):1870-1874
[38]Pandiarajan J,Krishnan M.Comparative bacterial survey in the gut of lepidopteran insects with different bionetwork[J].Microbiology,2018,87(1):103-115
[39]Demirci M,Sevim E,Demir I,et al.Culturable bacterial microbiota of Plagiodera versicolora(L.)(Coleoptera:Chrysomelidae)and virulence of the isolated strains[J].Folia Microbiologica,2013,58(3):201-210
[40]Hinojosa VS,Asenjo J,Leiva S.Agarolytic culturable bacteria associated with three antarctic subtidal macroalgae[J].World Journal of Microbiology&Biotechnology,2018,34(6):73
[41]Paniagua Voirol LR,Frago E,Kaltenpoth M,et al.Bacterial symbionts in Lepidoptera:Their diversity,transmission,and impact on the host[J].Frontiers in Microbiology,2018,9:556
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