贵州六盘水地区“红阳”猕猴桃软腐病病原菌分离鉴定及致病力差异测定
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摘要
以2016—2017年采集的"红阳"猕猴桃病果为试材,通过病原菌分离、病原菌鉴定及致病力测定,研究了"红阳"猕猴桃软腐病病原菌种类及其致病力差异,以期为"红阳"猕猴桃软腐病防控提供参考依据。结果表明:分离得到的23株病原菌,获得登录号分别为MF498063～498065,MG775300～775302和MG775283～775299;病原菌分属葡萄座腔菌Botryosphaeria sp.、交链孢菌Alternaria sp.、拟茎点霉菌Phomopsis sp.、小新壳梭孢菌Neofusicoccum sp.及间座壳菌Diaporthe sp.,各菌属检出率为30.4%、30.4%、26.1%、8.7%及4.4%;聚类分析将23株病原菌致病力分为弱致病力(26.09%)、中致病力(39.13%)、强致病力(26.09%)和较强致病力(8.69%)。
From 2016 to 2017,taking ripe rot kiwifruit collected from planting area in Guizhou Province Liupanshui city as raw material,and then the pathogens and pathogenicity differences were studied by pathogen isolation,pathogen identification,and pathogenicity identification in order to supply references for ripe rot prevention.The results showed that 23 pathogenic bacteria were isolated and obtained accession numbers MF498063-498065,MG775300-775302 and MG775283-775299,respectively the isolated pathogens were Botryosphaeria sp.,Alternaria sp.,Phomopsis sp.,Neofusicoccumsp.and Diaporthe sp.,the detection rates of respective genus were 30.4%,30.4%,26.1%,8.7% and4.4%;23 pathogenic bacteria experienced cluster analysis were divided into weak pathogenicity(26.09%),moderate pathogenicity(39.13%),strong pathogenicity(26.09%) and stronger pathogenicity(8.69%).
From 2016 to 2017,taking ripe rot kiwifruit collected from planting area in Guizhou Province Liupanshui city as raw material,and then the pathogens and pathogenicity differences were studied by pathogen isolation,pathogen identification,and pathogenicity identification in order to supply references for ripe rot prevention.The results showed that 23 pathogenic bacteria were isolated and obtained accession numbers MF498063-498065,MG775300-775302 and MG775283-775299,respectively the isolated pathogens were Botryosphaeria sp.,Alternaria sp.,Phomopsis sp.,Neofusicoccumsp.and Diaporthe sp.,the detection rates of respective genus were 30.4%,30.4%,26.1%,8.7% and4.4%;23 pathogenic bacteria experienced cluster analysis were divided into weak pathogenicity(26.09%),moderate pathogenicity(39.13%),strong pathogenicity(26.09%) and stronger pathogenicity(8.69%).
引文
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[19] 李媛,石凌波,费诺亚,等.蓝莓间座壳茎溃疡病病原鉴定及生物学特性研究[J].植物保护,2017,43(1):89-94.
[20] PHILIP R P.Development of resistance to picking wound entry Botrytis cinema storage rots in kiwifruit[J].New Zealand Journal of Experimental Agriculture,1994,22(4):387-392
[21] ZHU Y,DONG W,MA J,et al.Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China[J].BMC Veterinary Research,2017,13(1):94.
[22] 黄征宇,李传道.杨树溃疡病菌致病力分化及杨树抗病性评价[J].南京林业大学学报(自然科学版),1987(1):25-34.
[23] 张星耀,赵嘉平,梁军,等.树木枝干溃疡病菌致病力分化研究[J].中国森林病虫,2008,27(1):1-4.
[24] 张艳杰,许换平,沈凤英,等.我国葡萄灰霉病菌形态型和致病力分化[J].农业生物技术学报,2017,25(11):1740-1755.
[2] 王瑞玲.红阳猕猴桃采后病害生理及臭氧保鲜技术研究[D].雅安:四川农业大学,2010.
[3] HAWTHORNE B T.Fungi associated with leaf spots and post-harvest fruit rots of kiwifruit (Actinidia chinensis) in New Zealand[J].New Zealand Journal of Botany,1982,20(2):143-150.
[4] PENNYCOOK S R.Fungal fruit rots of Actinidia deliciosa (kiwifruit)[J].New Zealand Journal of Experimental Agriculture,1985,13 (4):289-299.
[5] LATORRE B A,ALVAREZ C,RIBEIRO O K.Phytophthora root rot of kiwifruit in Chile[J].Plant Disease,1991,75(9):949.
[6] LUONG L O.Phomopsis sp.associated with post-harvest fruit rot of kiwifruit in Italy[J].Journal of Plant Pathology,2011,93(1):205-209.
[7] 李黎,陈美艳,张鹏,等.猕猴桃软腐病的病原菌鉴定[J].植物保护学报,2016,43(3):527-528.
[8] PENNYCOOK S R,SAMUELS G J.Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand[J].Mycotaxon,1986(24):445-458.
[9] YOUNG J K.Postharvest fruit rots of kiwifruit (Actinidia deliciosa) in Korea[J].New Zealand Journal of Experimental Agriculture,2005,33(3):303-310.
[10] YOU Z,GONG G S,CUI Y L,et al.Identification of Botryosphaeriaceae species causing kiwifruit rot in Sichuan Province,China[J].Plant Disease,2015,99(5):150204092345006.
[11] 李黎,陈美艳,张鹏,等.猕猴桃果实软腐病菌鉴定及抗性品种初步筛选[C]//中国植物病理学会2015年学术年会论文集.2015.
[12] 周游.猕猴桃软腐病病原学研究[D].雅安:四川农业大学,2016.
[13] 王小洁,李士谣,李亚巍,等.猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J].植物保护学报,2017,44(5):826-832.
[14] 邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京:中国林业出版社,1984.
[15] SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(16):6241-6246.
[16] KUMAR S,STECHER G,TAMURA K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets.[J].Molecular Biology & Evolution,2016,33(7):1870.
[17] IVICA L,PEER B.Interactive tree of life (iTOL) v3:An online tool for the display and annotation of phylogenetic and other trees[J].Nucleic Acids Research,2016,44(Web Server issue):242-245.
[18] 程燕林,梁军,吕全,等.葡萄座腔菌科研究进展-鉴定,系统发育学和分子生态学[J].生态学报,2011,31(11):3197-3207.
[19] 李媛,石凌波,费诺亚,等.蓝莓间座壳茎溃疡病病原鉴定及生物学特性研究[J].植物保护,2017,43(1):89-94.
[20] PHILIP R P.Development of resistance to picking wound entry Botrytis cinema storage rots in kiwifruit[J].New Zealand Journal of Experimental Agriculture,1994,22(4):387-392
[21] ZHU Y,DONG W,MA J,et al.Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China[J].BMC Veterinary Research,2017,13(1):94.
[22] 黄征宇,李传道.杨树溃疡病菌致病力分化及杨树抗病性评价[J].南京林业大学学报(自然科学版),1987(1):25-34.
[23] 张星耀,赵嘉平,梁军,等.树木枝干溃疡病菌致病力分化研究[J].中国森林病虫,2008,27(1):1-4.
[24] 张艳杰,许换平,沈凤英,等.我国葡萄灰霉病菌形态型和致病力分化[J].农业生物技术学报,2017,25(11):1740-1755.