番茄果实色泽和色素含量的遗传特征
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摘要
番茄的颜色和色素含量是决定果实品质的两个重要特性。在本研究中,使用绿色和紫色番茄植株分别作为母本和父本,分别产生了6个遗传群体,即P1、P2、F1、BC1、BC2和F2。通过数量性状的多代联合分析研究果实颜色和色素含量的遗传规律。结果表明,绿色和紫色果之间的果实颜色的最佳遗传模型为MX1-AD-ADI。BC1、BC2和F2代色度值的主基因遗传率分别为82%、59%和77%,多基因遗传率分别为8%、10%和13%。番茄红素含量的遗传符合MX2-ADI-AD模型,BC1、BC2和F2的主基因遗传率分别为64%、74%和93%,而多基因的遗传率分别为20%、0%和0%。叶绿素的遗传模型为MX1-AD-ADI,BC1、BC2和F2的主基因遗传率分别为59%、74%和59%,多基因的遗传率均为0%。胡萝卜素的遗传符合MX2-ADI-ADI模型,BC1、BC2和F2的主基因遗传率分别为76%、4%/5%和54%,而多基因的遗传率分别为0%、0%和13%。
The color and pigment content of tomato are two important characteristics that determine fruit quality.In this study, green and purple tomato plants were used as female and male parents, respectively, to make six genetic groups, namely P1, P2, F1, BC1, BC2, and F2. The genetic rule of fruit color and pigment content was studied by multi-generation joint analysis of quantitative traits. The results showed that the best genetic model of fruit color between green and purple fruits was MX1-AD-ADI. The heritability of the major genes of BC1, BC2, and F2 generations was 82%, 59%, and 77%, respectively, while the heritability of multiple genes was 8%, 10%, and 13%, respectively. The inheritance of lycopene content was consistent with the MX2-ADI-AD model, the heritability of the major genes of BC1, BC2, and F2 were 64%, 74%, and 93%, respectively, whereas the heritability of multiple genes was 20%, 0%, and 0%, respectively. The genetic model of chlorophyll was MX1-AD-ADI, and the heritability of BC1, BC2, and F2 were 59%, 74%, and 59%, respectively. The heritability of multiple genes was 0%. The inheritance of carotene conformed to the MX2-ADI-ADI model, the heritability of the major genes of BC1, BC2,and F2 were 76%, 4%/5%, and 54%, respectively, while the heritability of multiple genes was 0%、0%, and 13% respectively.
The color and pigment content of tomato are two important characteristics that determine fruit quality.In this study, green and purple tomato plants were used as female and male parents, respectively, to make six genetic groups, namely P1, P2, F1, BC1, BC2, and F2. The genetic rule of fruit color and pigment content was studied by multi-generation joint analysis of quantitative traits. The results showed that the best genetic model of fruit color between green and purple fruits was MX1-AD-ADI. The heritability of the major genes of BC1, BC2, and F2 generations was 82%, 59%, and 77%, respectively, while the heritability of multiple genes was 8%, 10%, and 13%, respectively. The inheritance of lycopene content was consistent with the MX2-ADI-AD model, the heritability of the major genes of BC1, BC2, and F2 were 64%, 74%, and 93%, respectively, whereas the heritability of multiple genes was 20%, 0%, and 0%, respectively. The genetic model of chlorophyll was MX1-AD-ADI, and the heritability of BC1, BC2, and F2 were 59%, 74%, and 59%, respectively. The heritability of multiple genes was 0%. The inheritance of carotene conformed to the MX2-ADI-ADI model, the heritability of the major genes of BC1, BC2,and F2 were 76%, 4%/5%, and 54%, respectively, while the heritability of multiple genes was 0%、0%, and 13% respectively.
引文
Borghesi E.,Ferrante A.,Gordillo B.,Rodríguez-Pulido F.J.,Cocetta G.,and Trivellini A.,2016,Comparative physiology during ripening in tomato rich-anthocyanins fruits,Plant Growth Regulation,80(2):207-214
Causse M.,Buret M.,Robini K.,and Verschave P.,2010,Inheritance of nutritional and sensory quality traits in fresh market tomato and relation to consumer preferences,Journal of Food Science,68(7):2342-2350
Gonzali S.,Mazzucato A.,and Perata P.,2009,Purple as a tomato:towards high anthocyanin tomatoes,Trends Plant Sci.,14(5):237-241
Iii A.P.B.,Robertson L.D.,Labate J.A.,King B.A.,and King DE.,2015,Physicochemical and morphological analysis of ten tomato varieties identifies quality traits more readily manipulated through breeding and traditional selection methods,Journal of Food Composition&Analysis,42:16-25
Lin T.,Jin-Quan L.I.,Huang Q.F.,Chen C.W.,and Lin J.X.,2013,Inheritance of cherry tomato fruit color,Fujian Journal of Agricultural Sciences,28(10):987-992
Liu L.,Shao Z.,Zhan M.,and Wang Q.,2015,Regulation of carotenoid metabolism in tomato,Mol.Plant,8(1):28-39
Manoharan R.K.,Jung H.J.,Hwang I.,Jeong N.,Kho K.H.Chung M.Y.,and Nou I.S.,2017,Molecular breeding of a novel orange-brown tomato fruit with enhanced betacarotene and chlorophyll accumulation,Hereditas,154(1):1
Pan Q.,Liu X.X.,Peng H.R.,Pu Y.D.,Zhang Y.Z.,Ye S.H.,Wu G.T.,Qing L.,and Sun X.C.,2017,Cloning,expression ana lysis of Solanum lycopersicum SYTA,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(15):2936-2945(潘琪,刘旭旭,彭浩然,蒲运丹,张永至,叶思涵,吴根土,青玲孙现超,2017,番茄SYTA的克隆及表达分析,中国农业科学,50(15):2936-2945)
Panthee D.R.,Labate J.A.,Mcgrath M.T.,Api B.,and Robertson L.D.,2013,Genotype and environmental interaction for fruit quality traits in vintage tomato varieties,Euphytica,193(2):169-182
Peng H.R.,Pan Q.,Wei Z.L.,Pu Y.D.,Zhang Y.Z.,Wu G.T.,Qing L.,and Sun X.C.,2017,Cloning,expression and anti-virus function analysis of tomato resistance-related gene SIHin1,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(7):1242-1251(彭浩然,潘琪,魏周玲,蒲运丹,张永至,吴根土,青玲,孙现超,2017,番茄抗性相关基因SIHin1的克隆、表达与抗病毒功能,中国农业科学,50(7):1242-1251)
Rêgo E.R.D.,Fingeri F.L.,Casali V.W.D.,and Cardoso A.A.,1999,Inheritance of fruit color and pigment changes in a yellow tomato(Lycopersicon esculentum Mill.)mutant,Genet.Mol.Biol.,22(1):101-104
Richardson C.,and Hobson G.E.,2010,Compositional changes in normal and mutant tomato fruit during ripening and storage,Journal of the Science of Food&Agriculture,40(3):245-252
Zhang Y.,Gai J.,and Wang J.,2000,Identification of two major genes plus polygenes mixed inheritance model of quantitative traits in b_(1)and b_(2),and f_(2),Journal of Biomathematics,15(3):358-366
Zhang Y.,Zhao G.Y.,Li Y.S.,Zhang J.,Shi M.J.,Muhammad T.,and Liang Y.,2017,Transcriptome profiling of tomato uncovers an involvement of cytochrome p450s and peroxidases in stigma color formation,Front.Plant Sci.,8:897
Zhang Y.M.,and Gai J.,2009,Methodologies for segregation analysis and qtl mapping in plants,Genetica,136(2):311-318
Zhao X.,Liu Y.Z.,Wang J.,Li T.L.,and Sun Z.P.,2017,Effect of aeroponics on root growth and nutrient absorption in tomato,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),29(5):767-772(赵旭,刘艳芝,王静,李天来,孙周平,2017,雾培对番茄根系生长及其营养吸收的影响,浙江农业学报,29(5):767-772)
Causse M.,Buret M.,Robini K.,and Verschave P.,2010,Inheritance of nutritional and sensory quality traits in fresh market tomato and relation to consumer preferences,Journal of Food Science,68(7):2342-2350
Gonzali S.,Mazzucato A.,and Perata P.,2009,Purple as a tomato:towards high anthocyanin tomatoes,Trends Plant Sci.,14(5):237-241
Iii A.P.B.,Robertson L.D.,Labate J.A.,King B.A.,and King DE.,2015,Physicochemical and morphological analysis of ten tomato varieties identifies quality traits more readily manipulated through breeding and traditional selection methods,Journal of Food Composition&Analysis,42:16-25
Lin T.,Jin-Quan L.I.,Huang Q.F.,Chen C.W.,and Lin J.X.,2013,Inheritance of cherry tomato fruit color,Fujian Journal of Agricultural Sciences,28(10):987-992
Liu L.,Shao Z.,Zhan M.,and Wang Q.,2015,Regulation of carotenoid metabolism in tomato,Mol.Plant,8(1):28-39
Manoharan R.K.,Jung H.J.,Hwang I.,Jeong N.,Kho K.H.Chung M.Y.,and Nou I.S.,2017,Molecular breeding of a novel orange-brown tomato fruit with enhanced betacarotene and chlorophyll accumulation,Hereditas,154(1):1
Pan Q.,Liu X.X.,Peng H.R.,Pu Y.D.,Zhang Y.Z.,Ye S.H.,Wu G.T.,Qing L.,and Sun X.C.,2017,Cloning,expression ana lysis of Solanum lycopersicum SYTA,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(15):2936-2945(潘琪,刘旭旭,彭浩然,蒲运丹,张永至,叶思涵,吴根土,青玲孙现超,2017,番茄SYTA的克隆及表达分析,中国农业科学,50(15):2936-2945)
Panthee D.R.,Labate J.A.,Mcgrath M.T.,Api B.,and Robertson L.D.,2013,Genotype and environmental interaction for fruit quality traits in vintage tomato varieties,Euphytica,193(2):169-182
Peng H.R.,Pan Q.,Wei Z.L.,Pu Y.D.,Zhang Y.Z.,Wu G.T.,Qing L.,and Sun X.C.,2017,Cloning,expression and anti-virus function analysis of tomato resistance-related gene SIHin1,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(7):1242-1251(彭浩然,潘琪,魏周玲,蒲运丹,张永至,吴根土,青玲,孙现超,2017,番茄抗性相关基因SIHin1的克隆、表达与抗病毒功能,中国农业科学,50(7):1242-1251)
Rêgo E.R.D.,Fingeri F.L.,Casali V.W.D.,and Cardoso A.A.,1999,Inheritance of fruit color and pigment changes in a yellow tomato(Lycopersicon esculentum Mill.)mutant,Genet.Mol.Biol.,22(1):101-104
Richardson C.,and Hobson G.E.,2010,Compositional changes in normal and mutant tomato fruit during ripening and storage,Journal of the Science of Food&Agriculture,40(3):245-252
Zhang Y.,Gai J.,and Wang J.,2000,Identification of two major genes plus polygenes mixed inheritance model of quantitative traits in b_(1)and b_(2),and f_(2),Journal of Biomathematics,15(3):358-366
Zhang Y.,Zhao G.Y.,Li Y.S.,Zhang J.,Shi M.J.,Muhammad T.,and Liang Y.,2017,Transcriptome profiling of tomato uncovers an involvement of cytochrome p450s and peroxidases in stigma color formation,Front.Plant Sci.,8:897
Zhang Y.M.,and Gai J.,2009,Methodologies for segregation analysis and qtl mapping in plants,Genetica,136(2):311-318
Zhao X.,Liu Y.Z.,Wang J.,Li T.L.,and Sun Z.P.,2017,Effect of aeroponics on root growth and nutrient absorption in tomato,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),29(5):767-772(赵旭,刘艳芝,王静,李天来,孙周平,2017,雾培对番茄根系生长及其营养吸收的影响,浙江农业学报,29(5):767-772)