芍甘木瓜汤对脑卒中后痉挛大鼠神经递质的影响
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摘要
目的:实验采用芍甘木瓜汤灌胃脑卒中痉挛大鼠,以期为脑卒中术后患者痉挛的治疗提供参考。方法:60只大鼠分为对照组、假手术组、模型组和芍甘木瓜汤高、中、低剂量组,采用内囊电损毁法造模,电刺激法测定其偏瘫肢体伸直幅度,内囊中氨基酸类神经递质(谷氨酸、天门冬氨酸、甘氨酸和γ-氨基丁酸)采用高效毛细管电泳法检测,单胺类神经递质(5-羟色胺、多巴胺、去甲肾上腺素)采用荧光分光法检测。结果:与模型组比较,芍甘木瓜汤后脑卒有提高脑卒中痉挛大鼠偏瘫肢体伸直幅度和内囊中抑制性神经递质含量的作用,同时降低其内囊中单胺类神经递质含量。结论:对脑卒中痉挛大鼠模型灌胃的研究表明,芍甘木瓜汤可以通过改善脑受损后中枢神经系统中神经递质的代谢,抑制肌张力的提高,能有效缓解因脑卒中造成的肌痉挛现象。
Objective: In order to provide a reference for the treatment of convalescent spasm after stroke, the experiment used Shaogan Mugua decoction to treat spastic rats. Method: Muscle tension, amino acid neurotransmitters and monoamine neurotransmitters were detected. Result: Compared with the control group and sham-operated group, the muscle tension of the model rats increased significantly, the content of inhibitory neurotransmitters decreased significantly, and the content of excitatory neurotransmitters increased significantly. The inhibitory neurotransmitters increased, monoamine neurotransmitters decreased and muscle tension decreased significantly after gastric administration of sShaogan Mugua decoction. Conclusion: Shaogan Mugua decoction can improve the metabolism of neurotransmitters in the damaged central nervous system, inhibit the increase of muscle tension, and effectively alleviate the spasm caused by stroke.
Objective: In order to provide a reference for the treatment of convalescent spasm after stroke, the experiment used Shaogan Mugua decoction to treat spastic rats. Method: Muscle tension, amino acid neurotransmitters and monoamine neurotransmitters were detected. Result: Compared with the control group and sham-operated group, the muscle tension of the model rats increased significantly, the content of inhibitory neurotransmitters decreased significantly, and the content of excitatory neurotransmitters increased significantly. The inhibitory neurotransmitters increased, monoamine neurotransmitters decreased and muscle tension decreased significantly after gastric administration of sShaogan Mugua decoction. Conclusion: Shaogan Mugua decoction can improve the metabolism of neurotransmitters in the damaged central nervous system, inhibit the increase of muscle tension, and effectively alleviate the spasm caused by stroke.
引文
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[2] 张颖,杨坚,高宁沁.芍药甘草汤结合运动疗法治疗脑卒中后肘关节屈曲痉挛的临床研究田[J].上海中医药杂志,2012,46(11):47-48.
[3] 郑虎占,董泽宏,佘靖.中药现代研究与应用[M].北京:学苑出版社,1997:1256-1502.
[4] 唐世红,孙升云,姚奇.芍药甘草汤联合木瓜煎治疗小腿抽筋的临床体会[J].江西中医药大学学报,2018,30(3):13-14.
[5] 刘鸿燕,周奇志,蔡定均.构建大鼠内囊电毁损痉挛模型[J].四川生理科学杂志,2008,28(22):2214-2215.
[6] IA PIERRY,J ALIX,DG RAO.Longitudinal Diffusion-Weighted Whole-Body MRI Demonstrates Dynamic Changes in Muscle Integrity in Motor Neuron Disease[J].J.Neuromuscul Dis,2017,10.3233/JND-179002.
[7] VEVERKA T,HLU?TíK P,HOK P.Sensorimotor modulation by botulinum toxin A in post-stroke arm spasticity:Passive hand movement[J].Journal of the Neurological Sciences,2016,10.1016/j.jns,2015.12.049.
[8] 毛雪莲,秦思,金荣疆.电针对脑卒中肢体痉挛大鼠γ-氨基丁酸代谢酶的影响[J].中国康复医学杂志,2018,33(1):22-28.
[9] 王铖,王铖,刘风雨.在神经病理性疼痛大鼠中脊髓背角III层甘氨酸能神经元投射至II层放射状神经元的抑制性环路发生功能障碍[J].中国疼痛医学杂志,2017,23(2):100.
[10] 肖伟,章显宝,王震.针刺对卒中后抑郁大鼠脑组织神经递质及5-羟色胺转运体、5-羟色胺1A受体、去甲肾上腺素α2受体的影响[J].针刺研究,2016,41(6):528-534.