乙肝病毒血清标志物ECLIA法和ELISA法的比较分析
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摘要
目的比较ELISA法和ECLIA法检测乙肝血清标志物的检测性能。方法用ELISA法(酶联免疫吸附法)和ECLIA(电化学发光法)对HBV血清标志物进行检测,并对强阳性标本进行稀释。结果对120例患者的血清进行HBV五项的检测,ELISA法和ECLIA法对HBs Ag、HBs Ab、HBe Ag三项标志物的检出率无显著性差异(P>0.05),但对HBe Ab和HBc Ab的检测,电化学发光法检出率明显高于ELISA法(P<0.05)。结论乙肝病毒血清标志物的检测,ECLIA法检测血清乙肝标志物的灵敏度和自动化程度均高于ELISA法,并可动态观察疗效和监测病情。
Objective To compare the role of ELISA and electrochemiluminescence immunoassay( ECLIA) in detecting HBV serum markers. Methods ELISA and ECLIA methods were used to detect HBV serum markers,and dilution was conducted on strong positive samples. Results Five serum HBV markers were detected in 120 patients,and there was no significant difference in the detection rate of HBs Ag,Bs Ab and HBe Ag between ELISA and ECLIA( P > 0. 05),but for the detection rate of Hbe Ab and HBc Ab,ECLIA was higher than that of ELISA,( P < 0. 05). Conclusions For the detection of serum HBV markers,ECLIA is more sensitive and automatic than ELISA,and can be used to dynamically monitor clinical efficacy and disease progress.
Objective To compare the role of ELISA and electrochemiluminescence immunoassay( ECLIA) in detecting HBV serum markers. Methods ELISA and ECLIA methods were used to detect HBV serum markers,and dilution was conducted on strong positive samples. Results Five serum HBV markers were detected in 120 patients,and there was no significant difference in the detection rate of HBs Ag,Bs Ab and HBe Ag between ELISA and ECLIA( P > 0. 05),but for the detection rate of Hbe Ab and HBc Ab,ECLIA was higher than that of ELISA,( P < 0. 05). Conclusions For the detection of serum HBV markers,ECLIA is more sensitive and automatic than ELISA,and can be used to dynamically monitor clinical efficacy and disease progress.
引文
[1]程锦泉,马汉武,谢旭,等.深圳市常住人口乙型肝炎血清流行病学调查[J].中华流行病学杂志,2013,34(12):1179-1182.
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[7]唐中,张国元,凡瞿明,周彤,邓仁兵.1245例微粒子酶免化学发光分析技术检测乙肝两对半的结果分析[J].川北医学院学报,2003,18(3):83-84.
[8]Malik AH,Lee WM.Chronic hepatitis B virus infection:treatment strategies for the next millennium[J].Ann Inter Med,2000,132(9):723-731.
[9]中华医学会肝病学分会,中华医学会感染病学分会.慢性乙型肝炎防治指南[J].中国病原生物学杂志,2011,6(1):67-77.
[2]陶义训.免疫学和免疫学检验[M].北京:人民卫生出版社,2001,143-145.
[3]王岚,沈立松,等.不同方法检测乙型肝炎血清学标志物的结果研究[J].检验医学,2004,19(6):145-146.
[4]孙蓉,鲁军.电化学发光免疫分析法与酶联免疫吸附试验测定乙型肝炎标志物的比较与探讨[J].检验医学,2013,28(4):351-352.
[5]潘春琴,刘池波.微粒子酶免法检测HBV感染者血清学标志物的结果分析[J].江西医学检验,2002,7(3):69-70.
[6]许爱玲,林青,等.两种方法检测乙型肝炎血清学标志物的结果比较[J].实用医学杂志,2005,12(8):236-237.
[7]唐中,张国元,凡瞿明,周彤,邓仁兵.1245例微粒子酶免化学发光分析技术检测乙肝两对半的结果分析[J].川北医学院学报,2003,18(3):83-84.
[8]Malik AH,Lee WM.Chronic hepatitis B virus infection:treatment strategies for the next millennium[J].Ann Inter Med,2000,132(9):723-731.
[9]中华医学会肝病学分会,中华医学会感染病学分会.慢性乙型肝炎防治指南[J].中国病原生物学杂志,2011,6(1):67-77.