苦杏仁中苦杏仁苷的闪式提取工艺研究
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摘要
目的:研究闪式提取法从苦杏仁中提取苦杏仁苷的最佳工艺。方法:采用单因素试验和L9(34)正交试验设计,以苦杏仁苷为指标,对闪式提取的影响因素进行考察,并与文献报道的方法进行比较。结果:闪式提取法的最佳工艺是70%乙醇,提取时间1 min,料液比1∶6。结论:此工艺简单、高效、节能,提取率高,有利于苦杏仁苷的提取。
Objective: To study the optimal technology conditions of smashing extraction for extracting amygdalin form Semen Armeniacae Amarum. Methods: The influence factors on the smashing extraction were investigated by single-factor experiment and L9(34) orthogonal design taking the extraction rate of amygdalin as the index. And smashing extraction were compared with other methods reported in literature. Results: The optimal technique of the smashing extraction was as follows:70% ethanol was taken as solvent,the ratio of material and liquid was 1: 6,extracting time was 1 minute. Conclusion: The extraction process is simple, efficient, energy saving and with high extraction rate, which is beneficial to the extraction of amygdalin.
Objective: To study the optimal technology conditions of smashing extraction for extracting amygdalin form Semen Armeniacae Amarum. Methods: The influence factors on the smashing extraction were investigated by single-factor experiment and L9(34) orthogonal design taking the extraction rate of amygdalin as the index. And smashing extraction were compared with other methods reported in literature. Results: The optimal technique of the smashing extraction was as follows:70% ethanol was taken as solvent,the ratio of material and liquid was 1: 6,extracting time was 1 minute. Conclusion: The extraction process is simple, efficient, energy saving and with high extraction rate, which is beneficial to the extraction of amygdalin.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015.
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[3]李寅超,郭琰,张金艳.苦杏仁和桔梗平喘作用的配伍研究[J].中药药理与临床,2012,28(2):111-114
[4]Deng Jiagang,Li Chunyang,Wang Hailian,et al.Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory Tcells[J].Biochem Biophys Res Commun,2011,411(3):523-529.
[5]蔡莹,李运曼,钟流.苦杏仁苷对实验性胃溃疡的作用[J].中国药科大学学报,2003,34(3):254-256.
[6]海科,宋福成,周欣,等.苦杏仁苷对博莱霉素诱导的大鼠肺纤维化血清蛋白标志物的影响[J].中华劳动卫生职业病杂志,2010,28(4):260-263.
[7]方伟蓉,李运曼,钟林霖.苦杏仁苷对佐剂性炎症影响的实验研究[J].中国临床药理学与治疗学,2004,9(3):289-293.
[8]郑巧玲,郭义曹,孙杰,等.苦杏仁苷促进人血T淋巴细胞早熟凝集染色体增殖的实验研究[J].环境与职业医学,2009,26(6):572-574.
[9]郭君其,盛明雄,谭建明,等.苦杏仁苷抑制大鼠肾脏纤维化的实验研究[J].实用医学杂志,2007,23(17):2628-2630.
[10]范学辉,张清安,刘梅,等.苦杏仁脱苦方法研究进展[J].食品工业科技,2014,35(7):396-399.
[11]杨炳川,方应权.马尾松松针苦杏仁苷的闪式提取研究[J].中成药,2014,36(6):1309-1311.
[12]孟庆举,刘晓谦,杨华,等.闪式提取技术的进展[J].中国实验方剂学杂志,2013,19(19):349-354.
[13]邓引梅,宋发军,崔永明,等.甘草叶苦杏仁苷提取工艺[J].中南民族大学学报(自然科学版),2008,27(1):41-43.
[14]邹小娟,谢和兵,钱芳.HPLC法测定苦杏仁中苦杏仁苷含量的方法研究[J].中国药事,2009,23(1):33-36.
[15]张守平,刘瑛丽,曹洪锦.苦杏仁3种提取工艺中苦杏仁苷提取率的比较[J].齐鲁药事,2010,29(11):656-657.
[16]王玥,杜守颖,吴清.白芍中芍药苷的闪式提取工艺研究[J].北京中医药大学学报,2013,36(12):845-847.
[17]李文烈,王一波,曹海芳.牛蒡子中牛蒡苷闪式提取的工艺研究[J].广西中医药,2015,38(2):75-78.
[18]邓康颖,谭晓梅,罗佳波.一种苦杏仁苷提取物的制备方法[J].中华中医药学刊,2010,28(10):2172-2174.
[19]张志军.正交试验法优选苦杏仁苷的提取工艺[J].中医药导报,2015,21(10):56-57.
[2]甘露.大鼠p EGFP-N1-BKβ1真核表达载体的构建及苦杏仁苷对支气管平滑肌细胞增殖的研究[D].武汉:华中科技大学,2007.
[3]李寅超,郭琰,张金艳.苦杏仁和桔梗平喘作用的配伍研究[J].中药药理与临床,2012,28(2):111-114
[4]Deng Jiagang,Li Chunyang,Wang Hailian,et al.Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory Tcells[J].Biochem Biophys Res Commun,2011,411(3):523-529.
[5]蔡莹,李运曼,钟流.苦杏仁苷对实验性胃溃疡的作用[J].中国药科大学学报,2003,34(3):254-256.
[6]海科,宋福成,周欣,等.苦杏仁苷对博莱霉素诱导的大鼠肺纤维化血清蛋白标志物的影响[J].中华劳动卫生职业病杂志,2010,28(4):260-263.
[7]方伟蓉,李运曼,钟林霖.苦杏仁苷对佐剂性炎症影响的实验研究[J].中国临床药理学与治疗学,2004,9(3):289-293.
[8]郑巧玲,郭义曹,孙杰,等.苦杏仁苷促进人血T淋巴细胞早熟凝集染色体增殖的实验研究[J].环境与职业医学,2009,26(6):572-574.
[9]郭君其,盛明雄,谭建明,等.苦杏仁苷抑制大鼠肾脏纤维化的实验研究[J].实用医学杂志,2007,23(17):2628-2630.
[10]范学辉,张清安,刘梅,等.苦杏仁脱苦方法研究进展[J].食品工业科技,2014,35(7):396-399.
[11]杨炳川,方应权.马尾松松针苦杏仁苷的闪式提取研究[J].中成药,2014,36(6):1309-1311.
[12]孟庆举,刘晓谦,杨华,等.闪式提取技术的进展[J].中国实验方剂学杂志,2013,19(19):349-354.
[13]邓引梅,宋发军,崔永明,等.甘草叶苦杏仁苷提取工艺[J].中南民族大学学报(自然科学版),2008,27(1):41-43.
[14]邹小娟,谢和兵,钱芳.HPLC法测定苦杏仁中苦杏仁苷含量的方法研究[J].中国药事,2009,23(1):33-36.
[15]张守平,刘瑛丽,曹洪锦.苦杏仁3种提取工艺中苦杏仁苷提取率的比较[J].齐鲁药事,2010,29(11):656-657.
[16]王玥,杜守颖,吴清.白芍中芍药苷的闪式提取工艺研究[J].北京中医药大学学报,2013,36(12):845-847.
[17]李文烈,王一波,曹海芳.牛蒡子中牛蒡苷闪式提取的工艺研究[J].广西中医药,2015,38(2):75-78.
[18]邓康颖,谭晓梅,罗佳波.一种苦杏仁苷提取物的制备方法[J].中华中医药学刊,2010,28(10):2172-2174.
[19]张志军.正交试验法优选苦杏仁苷的提取工艺[J].中医药导报,2015,21(10):56-57.