枳实药材乙醇提取物的化学成分研究
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摘要
目的:研究枳实药材乙醇提取物的化学成分。方法:采用硅胶柱、开放ODS柱、制备液相色谱、HW-40F凝胶柱、大孔树脂柱对枳实药材乙醇提取物进行分离纯化,根据理化性质和波谱(质谱、氢谱、碳谱)数据分析鉴定化合物结构。结果:从枳实药材乙醇提取物中分离得到8个化合物,分别鉴定为Rimboxo(1)、胸苷(2)、尿嘧啶(3)、环-(亮氨酸1-异亮氨酸2-丙氨酸3-苏氨酸4-甘氨酸5-苏氨酸6-苯丙氨酸7)(4)、环-(亮氨酸1-亮氨酸2-脯氨酸3-酪氨酸4-甘氨酸5-丝氨酸6-脯氨酸7)(5)、水合橙皮内酯-β-D-葡萄糖苷(6)、伞形花内酯(7)、里那醇苷(8)。结论:化合物1、2、3、8为首次从柑橘属植物中分离得到,化合物4、5、6为首次从枳实药材中分离得到,该研究为枳实药材质量评价奠定了一定基础。
OBJECTIVE:To study the chemical constituents of ethanol extract from Cirtus aurantium. METHODS:The ethanol extract of C. aurantium was isolated and purified by silica gel column, open ODS column, preparation liquid chromatography,HW-40 F gel column and macroporous resin column. The structure of compounds was analyzed and identified according to physicochemical properties and spectral data(mass spectrum,hydrogen spectrum,carbon spectrum). RESULTS:Eight compounds were isolated from ethanol extract of C. aurantium,i.e. Rimboxo(1),Thymidine(2),Uracil(3),Cyclo-(Leu1-Ile2-Ala3-Thr4-Gly5-Thr6-Phe7)(4),Cyclo-(Leu1-Leu2-Pro3-Tyr4-Gly5-Ser6-Pro7)(5),Meranzin hydrate-β-D-glucoside(6),Umbelliferone(7),Linaloyl glucoside(8). CONCLUSIONS:Compound 1,2,3,8 are isolated from Citrus L. for the first time,compound 4,5,6 were isolated from C. aurantium for the first time. The study lays the foundation for quality evaluation of C.aurantium.
OBJECTIVE:To study the chemical constituents of ethanol extract from Cirtus aurantium. METHODS:The ethanol extract of C. aurantium was isolated and purified by silica gel column, open ODS column, preparation liquid chromatography,HW-40 F gel column and macroporous resin column. The structure of compounds was analyzed and identified according to physicochemical properties and spectral data(mass spectrum,hydrogen spectrum,carbon spectrum). RESULTS:Eight compounds were isolated from ethanol extract of C. aurantium,i.e. Rimboxo(1),Thymidine(2),Uracil(3),Cyclo-(Leu1-Ile2-Ala3-Thr4-Gly5-Thr6-Phe7)(4),Cyclo-(Leu1-Leu2-Pro3-Tyr4-Gly5-Ser6-Pro7)(5),Meranzin hydrate-β-D-glucoside(6),Umbelliferone(7),Linaloyl glucoside(8). CONCLUSIONS:Compound 1,2,3,8 are isolated from Citrus L. for the first time,compound 4,5,6 were isolated from C. aurantium for the first time. The study lays the foundation for quality evaluation of C.aurantium.
引文
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[2]张明智,隋海娟,张玲玲,等.橙皮苷对功能性消化不良大鼠胃肠运动及Ghrelin表达的影响[J].中药药理与临床,2014,30(2):30-34.
[3]苏名媛,牛江龙,李林,等.川陈皮素的体外抑癌活性及其机制研究[J].中成药,2011,33(9):1479-1483.
[4]YAPA AC,CHANB KG,SIMB KS,et al.A new oxolane from Enterobacter cloacae[J].Nat Prod Res,2016,30(7):783-788.
[5]邓慧颖,邢建广,罗都强.海莲内生真菌Pestalotiopsis clavispora代谢产物研究[J].菌物学报,2011,30(2):263-267.
[6]孙变娜,沈和定,吴洪喜,等.紫色疣石磺化学成分的分离与鉴定[J].中国药房,2014,25(11):1019-1021.
[7]彭文文,黄茂波,宋卫武,等.中药枳壳中环肽成分的研究[J].天然产物研究与开发,2014,26(9):1416-1420.
[8]MCHALE D,KHOPKAR PP,SHERIDAN JB.Coumarin glycosides from Citrus flavedo[J].Phytochemistry,1987,26(9):2547-2549.
[9]邓可众,丁邑强,周斌,等.枳壳化学成分的分离与鉴定[J].中国实验方剂学杂志,2015,21(14):36-38.
[10]SEO Y,SHIN J,CHA HJ,et al.A new monoterpene glucoside from portulaca oleracea[J].Cheminform,2004,35(10):1475-1477.