超高效液相色谱法测定有色稻米中花色苷的含量
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摘要
采用盐酸甲醇溶液超声提取有色稻米花色苷,以超高效液相色谱-紫外检测器对有色稻米中主要花色苷矢车菊素-3-O-葡萄糖苷和芍药素-3-O-葡萄糖苷进行定量检测。结果表明,在0. 5～50. 0μg/mL浓度范围内线性关系良好,R~2> 0. 999;矢车菊素-3-O-葡萄糖苷回收率在93. 0%～98. 5%,RSD在0. 33%～3. 50%;芍药素-3-O-葡萄糖苷回收率在96. 0%～111. 7%,RSD在0. 42%～2. 18%。对四川重庆地区的4个黑米样品、1个紫米样品和5个红米样品的糙米和米糠中主要花色苷的测定结果表明,黑米、黑米糠、紫米和紫米糠中花色苷总量分别为0～1 051. 27 mg/kg,74. 06～206. 82 mg/kg,296. 18 mg/kg和116. 15 mg/kg;红米中未检出矢车菊素-3-O-葡萄糖苷和芍药素-3-O-葡萄糖苷,部分红米糠中检出矢车菊素-3-O-葡萄糖苷,含量为0～50. 94 mg/kg。
Anthocyanins from colored rice were extracted with hydrochloric acid-methanol solutions under ultrasonic wave extraction. The detection of cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride in brown rice and rice bran were carried out by UHPLC-UV visible detector. The samples were four black rice,one purple rice and five red rice collected in Sichuan and Chongqing provinces. The results showed that the method was well linearly correlated in the concentration range of 0. 5 to 50 μg/mL for anthocyanins( R~2> 0. 999); the recoveries of cyandin-3-O-glucoside chloride were 93. 0%-98. 5% with relative standard deviations( RSD) of 0. 33%-3. 50%; the recoveries of peonidin-3-O-glucoside chloride were 96. 0%-111. 7% with RSD of 0. 42%-2. 18%. The total amount of anthocyanins in black rice,black rice bran,purple rice and purple rice bran was 0-1 051. 27,74. 06-206. 82,296. 18 and 116. 15 mg/kg respectively. Cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride were not detected in red rice,but cyandin-3-O-glucoside chloride was detected in some red rice bran samples,and the content was from 0 to 50. 94 mg/kg.
Anthocyanins from colored rice were extracted with hydrochloric acid-methanol solutions under ultrasonic wave extraction. The detection of cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride in brown rice and rice bran were carried out by UHPLC-UV visible detector. The samples were four black rice,one purple rice and five red rice collected in Sichuan and Chongqing provinces. The results showed that the method was well linearly correlated in the concentration range of 0. 5 to 50 μg/mL for anthocyanins( R~2> 0. 999); the recoveries of cyandin-3-O-glucoside chloride were 93. 0%-98. 5% with relative standard deviations( RSD) of 0. 33%-3. 50%; the recoveries of peonidin-3-O-glucoside chloride were 96. 0%-111. 7% with RSD of 0. 42%-2. 18%. The total amount of anthocyanins in black rice,black rice bran,purple rice and purple rice bran was 0-1 051. 27,74. 06-206. 82,296. 18 and 116. 15 mg/kg respectively. Cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride were not detected in red rice,but cyandin-3-O-glucoside chloride was detected in some red rice bran samples,and the content was from 0 to 50. 94 mg/kg.
引文
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[13]梁征,熊思慧,郭莹,等.紫米蛋白-多酚复合物的结构组成及分子形貌[J].食品科学,2016,37(21):85-90.
[14]闫亚美,代彦满,冉林武,等.黑果枸杞与5种果蔬中花色苷组成及体外抗氧化活性比较[J].食品工业科技,2014,35(16):133-136.
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[17]张晓芳,高伟,邓慧颖,等.红米红中矢车菊-3-葡萄糖苷含量与色价的相关性分析[J].食品科学,2013,34(20):135-138.
[18]胡莉,仲伶俐,毛建霏,等.高效液相色谱测定粮食、蔬菜和水果中的花青素[J].分析试验室,2012,31(12):43-47.
[19]夏效东,凌文华,郑琳,等. HPLC法测定黑米皮提取物中花色苷成分及含量[J].食品科学,2006,27(2):206-208.
[20]林丽,李进,丁成丽,等.高效液相色谱法测定黑果枸杞果实中花色苷的含量[J].食品科学,2013,34(6):164-166.
[21]赵则胜.中国特种稻[M].上海:上海科学技术出版社,1995:34-36.
[22]张名位.特种稻米及其加工技术[M].北京:中国轻工业出版社,2000:34-89.
[23]陈廷文.稻谷黑米种皮的遗传特性研究[J].四川农业大学学报,1995(4):498-505.
[24] RYU S N,PARK S Z,HO C T. High performance liquid chromatographic determination of anthocyanin pigments in some varieties of black rice[J]. Journal of food and Drug Analysis,1998,6(4):729-736.
[25] YAWADIO R,TANIMORI S,MORITA N. Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities[J]. Food Chemistry,2007,101(4):1 616-1 625.
[26]孔令瑶,汪云,曹玉华,等.黑米色素的组成与结构分析[J].食品与生物技术学报,2008,27(2):25-29.
[2] WANG H,CAO G,PRIOR R L. Oxygen radical absorbing capacity of anthocyanins[J]. Journal of Agricultural and Food Chemistry,1997,45(2):304-309.
[3] PROTEGGENTE A R,PANNALA A S,PAGANGA G,et al. The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition[J]. Free Radical Research,2002,36(2):217-233.
[4] SEERAM N P,MOMIN R A,NAIR M G,et al. Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries[J]. Phytomedicine,2001,8(5):362-369.
[5] YOSHIMOTO M,OKUNO S,KUMAGAI T,et al. Distribution of antimutagenic components in colored sweetpotatoes[J]. Japan Agricultural Research Quarterly,1999,33(3):143-148.
[6] HAGIWARA A,MIYASHITA K,NAKANISHI T,et al.Pronounced inhibition by a natural anthocyanin, purple corn color,of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(Ph IP)-associated colorectal carcinogenesis in male F344 rats pretreated with 1,2-dimethylhydrazine[J].Cancer Letters,2001,171(1):17-25.
[7] AUGER C,CAPORICCIO B,LANDRAULT N,et al. Red wine phenolic compounds reduce plasma lipids and apolipoprotein B and prevent early aortic atherosclerosis in hypercholesterolemic golden syrian hamsters(Mesocricetus auratus)[J]. The Journal of Nutrition,2002,132(6):1 207-1 213.
[8] JANKOWSKI A,JANKOWSKA B,NIEDWOROK J. Theeffect of anthocyanin dye from grapes on experimental diabetes[J]. Folia Medica Cracoviensia,1999,41(3/4):5-15.
[9] WANG H,NAIR M G,STRASBURG G M,et al. Antioxidant and antiinflammatory activities of anthocyanins and their aglycon,cyanidin,from tart cherries[J]. Journal of natural products,1999,62(2):294-296.
[10] MATSUMOTO H,INABA H,KISHI M,et al. Orally administered delphinidin 3-rutinoside and cyanidin 3-rutinoside are directly absorbed in rats and humans and appear in the blood as the intact forms[J]. Journal of Agricultural and Food Chemistry,2001,49(3):1 546-1 551.
[11] TAKAHASHI A,TAKEDA K,OHNISHI T. Light-induced anthocyanin reduces the extent of damage to DNA in UV-irradiated Centaurea cyanus cells in culture[J].Plant and Cell Physiology,1991,32(4):541-547.
[12]郭红辉,王庆,秦玉,等.液质联用分析黑米皮提取物中花色苷化合物[J].韶关学院学报,2007,28(9):77-80.
[13]梁征,熊思慧,郭莹,等.紫米蛋白-多酚复合物的结构组成及分子形貌[J].食品科学,2016,37(21):85-90.
[14]闫亚美,代彦满,冉林武,等.黑果枸杞与5种果蔬中花色苷组成及体外抗氧化活性比较[J].食品工业科技,2014,35(16):133-136.
[15]应龙彬,俞桑桑,刘催萍,等.高效液相色谱法对黑米中花色苷成分的测定[J].杭州师范大学学报(自然科学版),2012,11(1):28-32.
[16]郭红辉,王庆,秦玉,等.液质联用分析黑米皮提取物中花色苷化合物[J].韶关学院学报,2007,28(9):77-80.
[17]张晓芳,高伟,邓慧颖,等.红米红中矢车菊-3-葡萄糖苷含量与色价的相关性分析[J].食品科学,2013,34(20):135-138.
[18]胡莉,仲伶俐,毛建霏,等.高效液相色谱测定粮食、蔬菜和水果中的花青素[J].分析试验室,2012,31(12):43-47.
[19]夏效东,凌文华,郑琳,等. HPLC法测定黑米皮提取物中花色苷成分及含量[J].食品科学,2006,27(2):206-208.
[20]林丽,李进,丁成丽,等.高效液相色谱法测定黑果枸杞果实中花色苷的含量[J].食品科学,2013,34(6):164-166.
[21]赵则胜.中国特种稻[M].上海:上海科学技术出版社,1995:34-36.
[22]张名位.特种稻米及其加工技术[M].北京:中国轻工业出版社,2000:34-89.
[23]陈廷文.稻谷黑米种皮的遗传特性研究[J].四川农业大学学报,1995(4):498-505.
[24] RYU S N,PARK S Z,HO C T. High performance liquid chromatographic determination of anthocyanin pigments in some varieties of black rice[J]. Journal of food and Drug Analysis,1998,6(4):729-736.
[25] YAWADIO R,TANIMORI S,MORITA N. Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities[J]. Food Chemistry,2007,101(4):1 616-1 625.
[26]孔令瑶,汪云,曹玉华,等.黑米色素的组成与结构分析[J].食品与生物技术学报,2008,27(2):25-29.