高唐栝楼茎尖分生组织培养与快速繁殖技术研究
|
摘要
[目的]采用茎尖分生组织培养脱毒技术培育高唐栝楼的脱病毒试管苗。[方法]对添加不同浓度外源生长调节剂的培养基进行一系列优化试验,筛选出高唐栝楼脱毒试管苗最佳繁殖培养基。[结果]在MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0.10 mg/L KT培养基中诱导效果较好,技术上达到了快速繁殖规模生产的要求。经反复的病毒检测,证明脱毒试管苗脱病毒彻底。[结论]最终获得了高唐栝楼茎尖培养脱毒与诱导、增殖和生根的培养基,及其生长所需要的外部环境条件和相关配套技术。
[Objective]Virus-free seedling in test tube of Trichosanthes kirilowii Maxim. was obtained from stem-apex meristem culture in vitro. [Method]A series of optimization experiments for propagative medium composition and concentration of phytohormones were investigated with a view to accelerate the propagation of virus-free seedling in test tube of T. kirilowii Maxim. [Result]The most suitable rapid propagative medium of T. kirilowii Maxim. was MS + 2. 0 mg/L 6-BA + 0. 1 mg/L NAA + 0. 10 mg/L KT. These results could meet the technology requirements of rapid propagation in large scale production. Virus-free seedlings in test tube were tested repeatedly,which had no virus completely. [Conclusion]The cultural mediums of detoxification and induction,proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and matching technology.
[Objective]Virus-free seedling in test tube of Trichosanthes kirilowii Maxim. was obtained from stem-apex meristem culture in vitro. [Method]A series of optimization experiments for propagative medium composition and concentration of phytohormones were investigated with a view to accelerate the propagation of virus-free seedling in test tube of T. kirilowii Maxim. [Result]The most suitable rapid propagative medium of T. kirilowii Maxim. was MS + 2. 0 mg/L 6-BA + 0. 1 mg/L NAA + 0. 10 mg/L KT. These results could meet the technology requirements of rapid propagation in large scale production. Virus-free seedlings in test tube were tested repeatedly,which had no virus completely. [Conclusion]The cultural mediums of detoxification and induction,proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and matching technology.
引文
[1]李爱峰,张永清.栝楼的化学成分研究进展[J].安徽农业科学,2012,40(30):14713-14716.
[2]蔡宣梅,郭文杰,张洁,等.福建省道地瓜蒌组织培养体系的建立[J].福建热作科技,2016,41(2):1-3.
[3]邵春月,高山林,陈峰,等.怀地黄分生组织培养脱病毒及快速繁殖技术的研究[J].药物生物技术,2008,15(4):258-261.
[4]邓才生.人参果脱毒培养与快速繁殖研究[J].云南农业科技,2009(2):14-15.
[5]冯果,曾志乾,吴增光,等.基于综合评分法的不同产地加工方法对瓜蒌药材质量的影响[J].安徽农业科学,2017,45(29):120-123.
[6]韩琳娜,聂金娥,周凤琴,等.栝楼丛生芽快繁体系的建立[J].山东农业科学,2014,46(3):46-48.
[7]崔必波,王伟义,顾闽峰,等.瓜蒌套种麦子节本高产栽培技术初探[J].安徽农业科学,2015,43(28):75-76,101.
[8]马维平,黄连超,顾红卫,等.荆半夏组织培养及快速繁殖的研究[J].西北药学杂志,2006,21(2):57-59.
[2]蔡宣梅,郭文杰,张洁,等.福建省道地瓜蒌组织培养体系的建立[J].福建热作科技,2016,41(2):1-3.
[3]邵春月,高山林,陈峰,等.怀地黄分生组织培养脱病毒及快速繁殖技术的研究[J].药物生物技术,2008,15(4):258-261.
[4]邓才生.人参果脱毒培养与快速繁殖研究[J].云南农业科技,2009(2):14-15.
[5]冯果,曾志乾,吴增光,等.基于综合评分法的不同产地加工方法对瓜蒌药材质量的影响[J].安徽农业科学,2017,45(29):120-123.
[6]韩琳娜,聂金娥,周凤琴,等.栝楼丛生芽快繁体系的建立[J].山东农业科学,2014,46(3):46-48.
[7]崔必波,王伟义,顾闽峰,等.瓜蒌套种麦子节本高产栽培技术初探[J].安徽农业科学,2015,43(28):75-76,101.
[8]马维平,黄连超,顾红卫,等.荆半夏组织培养及快速繁殖的研究[J].西北药学杂志,2006,21(2):57-59.