红外光谱法结合判别分析法对抗风湿类中成药中替诺昔康的快速筛查
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摘要
以30种市售抗风湿类中成药作为试验样品,并从中选取不含替诺昔康的15种样品作为阴性样品,每种阴性样品各取0.2g左右,分别用甲醇10 mL超声提取,提取液经过滤后即得15种阴性样品溶液。取上述阴性样品15种,每种0.2g左右,3份,依次加入替诺昔康10,15,20mg,按上述方法用甲醇10mL提取,制得45种阳性样品溶液。称取30种试验样品,每种0.2g左右,按上述方法用甲醇10mL提取,制得30种待测样品溶液。分别移取上述样品溶液各5μL,采集其红外光谱图。用TQ Analyst 9.0软件及所得红外光谱图,按判别分析法建立模型,可对抗风湿类中成药中是否添加替诺昔康进行快速筛查。结果表明:所建模型的误判数为0,正确识别率为100%。
Thirty kinds of anti-rheumatic Chinese patent drugs sold on market were taken as testing samples,and 15 of which,proved to contain no tenoxicam,were selected as the negative samples.Portions of about 0.2 g were taken from each of the negative samples,and were extracted ultrasonically with 10 mL of methanol separately.After filtering,the solution of the 15 negative samples were thus obtained.Three portions(about 0.2 g each)of each of the 15 negative samples were taken and 10,15,20 mg of tenoxicam were added respectively.These samples were extracted each with 10 mL methanol as described for the negative samples.Thus the solution of 45 positive samples were obtained.About 0.2 g sample portion was then taken from each of the 30 testing samples and extracted with 10 mL of methanol as described for the negative samples.Thus the solution from the 30 testing samples were obtained.Aliquots of 5μL were taken from each of the above described solution and their IR spectra were taken.By using the soft ware TQ Analyst 9.0 and based on the IR spectra,discriminant analysis was applied to obtain the model for rapid screening of tenoxicam admixed into the anti-rheumatic Chinese patent drugs.It was found that by using the established model,rate of correct recognition of 100% was attained,with misdeeming case of zero.
Thirty kinds of anti-rheumatic Chinese patent drugs sold on market were taken as testing samples,and 15 of which,proved to contain no tenoxicam,were selected as the negative samples.Portions of about 0.2 g were taken from each of the negative samples,and were extracted ultrasonically with 10 mL of methanol separately.After filtering,the solution of the 15 negative samples were thus obtained.Three portions(about 0.2 g each)of each of the 15 negative samples were taken and 10,15,20 mg of tenoxicam were added respectively.These samples were extracted each with 10 mL methanol as described for the negative samples.Thus the solution of 45 positive samples were obtained.About 0.2 g sample portion was then taken from each of the 30 testing samples and extracted with 10 mL of methanol as described for the negative samples.Thus the solution from the 30 testing samples were obtained.Aliquots of 5μL were taken from each of the above described solution and their IR spectra were taken.By using the soft ware TQ Analyst 9.0 and based on the IR spectra,discriminant analysis was applied to obtain the model for rapid screening of tenoxicam admixed into the anti-rheumatic Chinese patent drugs.It was found that by using the established model,rate of correct recognition of 100% was attained,with misdeeming case of zero.
引文
[1]刘松林.替诺昔康Tenoxicam[J].药学进展,1988,12(1):46-47.
[2]丁晨光.阿司匹林和替诺昔康[J].药学情报通讯,1989,7(2):34-35.
[3]蒋建敏,许实波.替诺昔康的解热、镇痛、抗炎作用[J].中山大学学报(自然科学版),1999,38(增刊1):104-107.
[4]张鹤,黄子桐,朱禹彭,等.关于抗风湿类中药制剂中非法添加化学药品的检测方法研究[J].亚太传统医药,2010,6(6):31-32.
[5]刘惠民,王仕平,刘卿.吡罗昔康与美洛昔康的快速检测[J].中国现代应用药学,2010,27(12):1130-1133.
[6]邓树勇,马启龙,丁燕,等.TLC法快速筛选抗风湿类中成药中的化学药品[J].药物分析杂志,2009,29(10):1719-1721.
[7]苏健.薄层色谱法检测抗风湿类中成药中解热镇痛非甾体抗炎药的非法添加[J].云南中医中药杂志,2013,35(5):74-74.
[8]黎雪清,谭渺,蒋创杰,等.HPLC法快速筛查抗风湿类中成药和保健食品中24种非法添加化学成分[J].中成药,2014,36(9):1891-1894.
[9]李存金,郭飞宇.HPLC检测抗风湿类中成药中非法添加非甾体类化学物质[J].中成药,2010,32(12):2191-2194.
[10]周建良,芳翠芬,陈勇,等.快速液相色谱-串联质谱联用同时检测抗风湿类中药制剂中13种添加化学药物成分[J].药物分析杂志,2013,33(2):281-285.
[11]李丹,文红梅,崔福春,等.LC-MS/MS法快速测定中成药、保健食品中非法添加的36种化学成分[J].药物分析杂志,2010,30(8):1527-1532.
[12]潘炜,顾鑫荣,刘志璋,等.LC-MS/MS法测定中成药制剂中23个非甾体抗炎药[J].药物分析杂志,2012,32(2):261-266.
[13]陈梓云,彭梦侠,梁奇峰,等.衰减全反射傅里叶红外光谱法建模快筛中成药“骨节灵”中的泼尼松龙和醋酸泼尼松龙[J].化学世界,2017,58(3):170-174.
[14]梅德强,王建华.红外光谱法研究中药进展[J].重庆大学学报(自然科学版),2003,26(11):56-59.
[15]周密,柯剑,李宝丽,等.傅里叶红外光谱结合化学计量学鉴别预包装纯菠萝汁中外源糖[J].光谱学与光谱分析,2015,35(10):2734-2739.
[16]王家俊,邱启杨,刘巍.FTIR-ATR指纹谱图的主成分分析-马氏距离法应用与烟用香精质量控制[J].光谱学与光谱分析,2007,27(5):895-898.
[2]丁晨光.阿司匹林和替诺昔康[J].药学情报通讯,1989,7(2):34-35.
[3]蒋建敏,许实波.替诺昔康的解热、镇痛、抗炎作用[J].中山大学学报(自然科学版),1999,38(增刊1):104-107.
[4]张鹤,黄子桐,朱禹彭,等.关于抗风湿类中药制剂中非法添加化学药品的检测方法研究[J].亚太传统医药,2010,6(6):31-32.
[5]刘惠民,王仕平,刘卿.吡罗昔康与美洛昔康的快速检测[J].中国现代应用药学,2010,27(12):1130-1133.
[6]邓树勇,马启龙,丁燕,等.TLC法快速筛选抗风湿类中成药中的化学药品[J].药物分析杂志,2009,29(10):1719-1721.
[7]苏健.薄层色谱法检测抗风湿类中成药中解热镇痛非甾体抗炎药的非法添加[J].云南中医中药杂志,2013,35(5):74-74.
[8]黎雪清,谭渺,蒋创杰,等.HPLC法快速筛查抗风湿类中成药和保健食品中24种非法添加化学成分[J].中成药,2014,36(9):1891-1894.
[9]李存金,郭飞宇.HPLC检测抗风湿类中成药中非法添加非甾体类化学物质[J].中成药,2010,32(12):2191-2194.
[10]周建良,芳翠芬,陈勇,等.快速液相色谱-串联质谱联用同时检测抗风湿类中药制剂中13种添加化学药物成分[J].药物分析杂志,2013,33(2):281-285.
[11]李丹,文红梅,崔福春,等.LC-MS/MS法快速测定中成药、保健食品中非法添加的36种化学成分[J].药物分析杂志,2010,30(8):1527-1532.
[12]潘炜,顾鑫荣,刘志璋,等.LC-MS/MS法测定中成药制剂中23个非甾体抗炎药[J].药物分析杂志,2012,32(2):261-266.
[13]陈梓云,彭梦侠,梁奇峰,等.衰减全反射傅里叶红外光谱法建模快筛中成药“骨节灵”中的泼尼松龙和醋酸泼尼松龙[J].化学世界,2017,58(3):170-174.
[14]梅德强,王建华.红外光谱法研究中药进展[J].重庆大学学报(自然科学版),2003,26(11):56-59.
[15]周密,柯剑,李宝丽,等.傅里叶红外光谱结合化学计量学鉴别预包装纯菠萝汁中外源糖[J].光谱学与光谱分析,2015,35(10):2734-2739.
[16]王家俊,邱启杨,刘巍.FTIR-ATR指纹谱图的主成分分析-马氏距离法应用与烟用香精质量控制[J].光谱学与光谱分析,2007,27(5):895-898.