十枣汤联合顺铂干预恶性腹水的作用机制研究
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摘要
目的:探讨十枣汤联合顺铂干预恶性腹水的作用机制。方法:将50只昆明种小鼠随机分为空白组、模型组、顺铂组、中药组、中药+顺铂组各10只。除空白组,其他各组复制艾氏腹水瘤模型,建模24 h后开始给药,给药10 d后处死小鼠,记录小鼠体质量,测量腹水量及脾和胸腺的质量,酶联免疫吸附测定(ELISA)法检测小鼠血清中白细胞介素2(IL-2)、干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、血管内皮生长因子(VEGF)含量。结果:给药干预后,与模型组比较,除空白组外,各治疗组腹水明显减少(P<0.01),中药+顺铂组效果最佳,IL-2、IFN-γ含量显著升高(P<0.01),TNF-α、VEGF含量显著降低(P<0.01)。结论:十枣汤与顺铂联用能够明显抑制恶性腹水,其机制可能与升高IL-2、IFN-γ含量及降低TNF-α、VEGF含量有关。
Objective:To investigate the mechanism of Shizao Decoction combined with cisplatin in the treatment of malignant ascites. Methods:Fifty Kunming mice were randomly divided into blank group,model group,cisplatin group,Chinese medicinal group,and Chinese medicinal + cisplatin group. Except the blank group,in all groups the Ehrlich ascites tumor model was established,and the drug was administered 24 hours after modeling. The mice were sacrificed 10 days after the administration,and the weight was recorded. The amount of ascites and the weight of the spleen and thymus were measured. The serum levels of interleukin-2(IL-2),interferon γ(IFN-γ),tumor necrosis factor α(TNF-α),and vascular endothelial growth factor(VEGF) were detected in mouse serum using enzyme linked immunosorbent assay(ELISA). Results:After intervention,compared with the model group,the ascites of each treatment group was significantly decreased(P <0.01),and the effect in the Chinese medicinal + cisplatin group was the best,IL-2 and IFN-γ levels were significantly increased(P<0.01),TNF-α and VEGF levels were significantly decreased(P<0.01). Conclusion:The combination of Shizao Decoction and cisplatin can significantly inhibit the development of malignant ascites. The mechanism may be related to the increase of IL-2 and IFN-γlevels and the decrease of TNF-α and VEGF levels.
Objective:To investigate the mechanism of Shizao Decoction combined with cisplatin in the treatment of malignant ascites. Methods:Fifty Kunming mice were randomly divided into blank group,model group,cisplatin group,Chinese medicinal group,and Chinese medicinal + cisplatin group. Except the blank group,in all groups the Ehrlich ascites tumor model was established,and the drug was administered 24 hours after modeling. The mice were sacrificed 10 days after the administration,and the weight was recorded. The amount of ascites and the weight of the spleen and thymus were measured. The serum levels of interleukin-2(IL-2),interferon γ(IFN-γ),tumor necrosis factor α(TNF-α),and vascular endothelial growth factor(VEGF) were detected in mouse serum using enzyme linked immunosorbent assay(ELISA). Results:After intervention,compared with the model group,the ascites of each treatment group was significantly decreased(P <0.01),and the effect in the Chinese medicinal + cisplatin group was the best,IL-2 and IFN-γ levels were significantly increased(P<0.01),TNF-α and VEGF levels were significantly decreased(P<0.01). Conclusion:The combination of Shizao Decoction and cisplatin can significantly inhibit the development of malignant ascites. The mechanism may be related to the increase of IL-2 and IFN-γlevels and the decrease of TNF-α and VEGF levels.
引文
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[13]赵钢,侯风刚.通络利水中药结合白细胞介素-2治疗消化系统肿瘤癌性腹水32例[J].上海中医药杂志,2008(5):29-30.
[14]姚天琦,王伏生,路晓庆,等.干扰素-γ在肿瘤免疫中的双重作用[J].中国药物与临床,2018,18(4):544-547.
[15]余家俊,郭永连,李国灏,等.血管内皮生长因子及肿瘤坏死因子-α在膀胱癌中的表达及其相关性研究[J].中国医学前沿杂志(电子版),2015,7(6):153-155.
[16]金志超,施展,花宝金.恶性胸腔积液的机制研究进展及中医药干预靶点的思考[J].世界中西医结合杂志,2017,12(1):11-17.
[17]曾贞,邢枫,周爱民,等.消胀贴膏外敷对小鼠肝硬化腹水的消退作用及对腹腔血管通透性的影响[J].临床肝胆病杂志,2012,28(9):689-694.
[2]王怀碧,江飞龙,赖宗浪,等.恶性腹水的治疗现状及进展[J].中国中医急症,2017,26(12):2162-2164.
[3]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004(9):1069-1072.
[4]王宝丽,邹迪新,程钱,等.京大戟化学成分及药理作用研究概述[J].环球中医药,2016,9(7):896-900.
[5]赵雪艳,蔡霞,胡正海.甘遂生物学、化学成分和药理作用研究进展[J].中草药,2014,45(20):3029-3033.
[6]李玲芝,宋少江,高品一.芫花的化学成分及药理作用研究进展[J].沈阳药科大学学报,2007,24(9):587-592.
[7]吴国泰,何小飞,牛亭惠,等.大枣的化学成分、药理及应用[J].中国果菜,2016,36(10):25-28.
[8]李航森,肖曼丽.十枣汤对抑制小鼠艾氏腹水的实验研究[J].中医药临床杂志,2012,24(8):771-773.
[9]毛庆琳.消瘤祛毒饮对H22肝癌小鼠瘤重、胸腺指数、脾指数及淋巴细胞转化功能的影响[D].长春:吉林大学,2013.
[10]王云飞,杨小明,李月英,等.银杏酚对SMMC-7721肝癌细胞和荷H22肝癌小鼠的抗癌作用[J].江苏大学学报:医学版,2013,23(3):234-237.
[11]赵定亮,单风平.白细胞介素-2最新研究进展[J].微生物学杂志,2013,33(4):77-83.
[12]陈林.联合检测IFN-γ、TNF-α和IL-2对结核性胸腔积液和恶性胸腔积液鉴别诊断的价值[J].基因组学与应用生物学,2018,37(2):570-575.
[13]赵钢,侯风刚.通络利水中药结合白细胞介素-2治疗消化系统肿瘤癌性腹水32例[J].上海中医药杂志,2008(5):29-30.
[14]姚天琦,王伏生,路晓庆,等.干扰素-γ在肿瘤免疫中的双重作用[J].中国药物与临床,2018,18(4):544-547.
[15]余家俊,郭永连,李国灏,等.血管内皮生长因子及肿瘤坏死因子-α在膀胱癌中的表达及其相关性研究[J].中国医学前沿杂志(电子版),2015,7(6):153-155.
[16]金志超,施展,花宝金.恶性胸腔积液的机制研究进展及中医药干预靶点的思考[J].世界中西医结合杂志,2017,12(1):11-17.
[17]曾贞,邢枫,周爱民,等.消胀贴膏外敷对小鼠肝硬化腹水的消退作用及对腹腔血管通透性的影响[J].临床肝胆病杂志,2012,28(9):689-694.