植物乳杆菌LPL10的体外益生特性研究
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摘要
[目的]在筛选到一株食品源植物乳杆菌LPL10的基础上,通过对其耐酸性、耐胆盐、抑菌能力和产酸能力进行研究,确定其益生特性。[方法]采用平板活菌计数法测定LPL10的耐酸、耐胆盐性能,通过抑菌圈法评价菌株的抑菌能力,高效液相色谱法测定LPL10发酵产有机酸能力。[结果]植物乳杆菌LPL10具有良好的低酸耐受能力,在pH 3.5条件下培养24 h活菌数为1.2×108 cfu/mL,存活率为148%;LPL10受0.1%胆盐抑制影响较小,培养24 h后活菌数为3.60×108 cfu/mL,存活率为356%;植物乳杆菌LPL10对指示菌金黄色葡萄球菌、藤黄微球菌和绿脓杆菌均有良好的抑制作用;LPL10发酵上清液中产的主要有机酸为乳酸和乙酸,相对含量分别为20.33、25.26 mg/L。[结论]体外益生特性研究表明,植物乳杆菌LPL10可以作为潜在的益生性菌株。
[Objective]The probiotic characteristics of Lactobacillus plantarum LPL10 obtained from food products were evaluated through acid tolerance, bile salt resistance, antibacterial activity and acid-producing ability. [Method]The resistance to acid and bile salt of LPL10 were researched by plate counting method, its antibacterial property by the inhibition zone method, the acid-producing ability by high performance liquid chromatography.[Result]Lactobacillus plantarum LPL10 had a good acid-lowering tolerance, and the viable count was 1.2×10~8 cfu/mL in 24 h at pH 3.5, the survival rate was 148%. There was low suppression on containing 0.1% bile salt for LPL10, the viable count was 3.60×10~8 cfu/mL after the culture of 24 h and its survival rate was 356%; LPL10 played an inhibitory role in Staphylococcus aureus, Micrococcus luteus,and Pseudomonas aeruginosa.Lactic acid and acetic acid were the main organic acids produced by fermenlatin broth of LPL10, the relative contents were 20.33 and 25.26 mg/L, respectively. [Conclusion]The research results showed that Lactobacillus plantarum LPL10 could be a potential probiotic strain.
[Objective]The probiotic characteristics of Lactobacillus plantarum LPL10 obtained from food products were evaluated through acid tolerance, bile salt resistance, antibacterial activity and acid-producing ability. [Method]The resistance to acid and bile salt of LPL10 were researched by plate counting method, its antibacterial property by the inhibition zone method, the acid-producing ability by high performance liquid chromatography.[Result]Lactobacillus plantarum LPL10 had a good acid-lowering tolerance, and the viable count was 1.2×10~8 cfu/mL in 24 h at pH 3.5, the survival rate was 148%. There was low suppression on containing 0.1% bile salt for LPL10, the viable count was 3.60×10~8 cfu/mL after the culture of 24 h and its survival rate was 356%; LPL10 played an inhibitory role in Staphylococcus aureus, Micrococcus luteus,and Pseudomonas aeruginosa.Lactic acid and acetic acid were the main organic acids produced by fermenlatin broth of LPL10, the relative contents were 20.33 and 25.26 mg/L, respectively. [Conclusion]The research results showed that Lactobacillus plantarum LPL10 could be a potential probiotic strain.
引文
[1] 王水泉,包艳,董喜梅,等.植物乳杆菌的生理功能及应用[J].中国农业科技导报,2010,12(4):49-55.
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[3] 黄秀敏,张宜靖,李学优,等.植物乳杆菌发酵培养基的优化[J].安徽农业科学,2016,44(24):6-7,22.
[4] LIU Y W,LIONG M T,CHIEH T Y.New perspectives of Lactobacillus plantarum as a probiotic: The gut-heart-brain axis[J]. J Microbiol,2018,56(9):601-613.
[5] 王春光,张雪,李达,等.酸菜来源植物乳杆菌的分离鉴定与耐受性研究[J].食品科技,2010,35(10):35-38.
[6] 唐雅茹,于上富,国立东,等.一株降胆固醇乳杆菌的筛选及其益生作用的研究[J].食品工业科技,2016,37(1):142-144,152.
[7] 侯颖,王维宇,牛明福,等.自然发酵泡菜汁中植物乳杆菌的分离鉴定与体外益生特性研究[J].动物营养学报,2017,29(10):3611-3619.
[8] 甘祥武,黄魁英,曹丁,等.3株植物乳杆菌LPL04、LPL05、LPL14的益生特性[J].食品科技,2017,42(12):2-5.
[9] 林龙镇,邹卫玲,李安章,等.产酸、耐酸乳酸菌的分离鉴定及益生特性[J].华南农业大学学报,2018,39(2):95-102.
[2] 靳志强,王延样.植物乳杆菌在人体肠道的益生特性[J].中国乳品工业,2007,35(9):30-34.
[3] 黄秀敏,张宜靖,李学优,等.植物乳杆菌发酵培养基的优化[J].安徽农业科学,2016,44(24):6-7,22.
[4] LIU Y W,LIONG M T,CHIEH T Y.New perspectives of Lactobacillus plantarum as a probiotic: The gut-heart-brain axis[J]. J Microbiol,2018,56(9):601-613.
[5] 王春光,张雪,李达,等.酸菜来源植物乳杆菌的分离鉴定与耐受性研究[J].食品科技,2010,35(10):35-38.
[6] 唐雅茹,于上富,国立东,等.一株降胆固醇乳杆菌的筛选及其益生作用的研究[J].食品工业科技,2016,37(1):142-144,152.
[7] 侯颖,王维宇,牛明福,等.自然发酵泡菜汁中植物乳杆菌的分离鉴定与体外益生特性研究[J].动物营养学报,2017,29(10):3611-3619.
[8] 甘祥武,黄魁英,曹丁,等.3株植物乳杆菌LPL04、LPL05、LPL14的益生特性[J].食品科技,2017,42(12):2-5.
[9] 林龙镇,邹卫玲,李安章,等.产酸、耐酸乳酸菌的分离鉴定及益生特性[J].华南农业大学学报,2018,39(2):95-102.