禾谷镰孢菌FgCYP51B蛋白Y137H影响分生孢子及子囊孢子发育
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摘要
<正>小麦赤霉病(Fusarium Head Blight,FHB),主要是由镰刀菌属真菌引起的一种流行性毁灭性病害。在世界各小麦种植区尤其是温暖潮湿和半潮湿地区发生最为严重~([1,2])。它不仅能够降低小麦产量,其染病麦粒中还会产生对人畜健康严重危害的真菌毒素~([3])。在不同的地区由于气候因素和地理环境的不同,分布着不同的致病菌株。在我国,
Tyrosine 137 is one of key residues in selective binding of DMIs to FgCYP51B( Fusarium graminearum CYP51B),which consists of a drug binding active pocket and plays an important role in the interaction between CYP51 and DMIs. In this study,we investigated the FgCYP51B Y137 H biological phenotypes on conidiation,female fertility,colony morphology,growth rate,and pathogenicity. We found that the Y137 H mutation of FgCYP51B showed a significantly reduction on conidiation and influences on development of ascospores compared with the wild-type strain. However,colony morphology,growth rate,and pathogenicity of the Y137H mutation of FgCYP51B were consistent with that of the wild-type strain. These results suggest that the FgCYP51B Y137H mutation has influences on conidiation and development of ascospores,but is not associated with colony morphology,growth rate and pathogenicity.
Tyrosine 137 is one of key residues in selective binding of DMIs to FgCYP51B( Fusarium graminearum CYP51B),which consists of a drug binding active pocket and plays an important role in the interaction between CYP51 and DMIs. In this study,we investigated the FgCYP51B Y137 H biological phenotypes on conidiation,female fertility,colony morphology,growth rate,and pathogenicity. We found that the Y137 H mutation of FgCYP51B showed a significantly reduction on conidiation and influences on development of ascospores compared with the wild-type strain. However,colony morphology,growth rate,and pathogenicity of the Y137H mutation of FgCYP51B were consistent with that of the wild-type strain. These results suggest that the FgCYP51B Y137H mutation has influences on conidiation and development of ascospores,but is not associated with colony morphology,growth rate and pathogenicity.
引文
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[14]Qian,H W,Duan M L,Sun X M,et al.The binding mechanism betw een azoles and FgCYP51B,sterol14α-demethylase of Fusarium graminearum[J].Pest M anagement Science,2017,74(1):126-134.
[15]Qian,H W,Du J,Chi M Y,et al.The Y137H mutation in the FgCYP51B protein confers reduced sensitivity to tebuconazole in Fusarium graminearum[J].Pest M anagement Science,2017,DOI:10.1002/ps.4837.
[16]Chen C J,Yu J J,Bi C W,et al.Mutations in aβ-tubulin confer resistance of G ibberella zeae to benzimidazole fungicides[J].Phytopathology,2009,99(19):1403-1411.
[17]Zhang H,Li B,Fang Q,et al.SNARE protein FgVam7 controls grow th,asexual and sexual development,and plant infection in Fusarium graminearum[J].Molecular Plant Pathology,2016,17(1):108-119.
[18]Zhu Y Y,Liang X Y,Duan Y B,et al.F240 ofβ2 tubulin explains w hy Fusarium graminearum is less sensitive to carbendazim than Botrytis cinerea[J].Phytopathology,2017,Available at doi:10.1094/PHYTO-09-17-0295-R.
[19]Fan J R.Investigating the function of three paralogous CYP51 gene in Fusarium graminearum[D].Beijing:China Agricultural University,2013.
[2]An X,Zhang H J,Jiang F S,et al.Occurrence and control of Fusarium Head blight in Wheat[J].M odern Agricultural Science and Technology,2015,(22):136-137.
[3]Kang Z,Buchenauer H.Studies on the infection process of Fusarium culmorum in w heat spikes:Degradation of hoet cell w all components and localization of trichothecene toxins in infected tissue[J].European Journal of Plant Pathology,2002,108(7):653-660.
[4]Zhang J B,Li H R,Dang F J,et a1.Determination of the trichothecene mycotoxin chemotypes and associatod geographical distribution and phyloganetic species of the Fusarium graminearum clade from China[J].M ycological Research,2007,111(8):967-975.
[5]Tóth B,Mesterházy A,Horváth,Z,et al.Genetic variability of central European isolates of the Fusarium graminearum species complex[J].European Journal of Plant Pathology,2005,113(1):35-45.
[6]Waalwijk C,Kastelein P,Vries I D,et al.Major changes in Fusarium spp.in w heat in the Netherlands[J].European Journal of Plant Pathology,2003,109(7):743-754.
[7]Dean R,Van Kan J A L,Pretorius Z A,et al.The top10 fungal pathogens in molecular plant pathology[J].M olecular Plant Pathology,2012,13(4):414-430.
[8]Chen C J,Yu J J,Bi C W,et al.Mutations in aβ-tubulin confer resistance of G ibberella zeae to benzimidazole fungicides[J].Pytopathology,2009,99(12):1403.
[9]Yin Y,Liu X,Li B,et al.Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F.graminearum collected from w heat in China[J].Phytopathology,2009,99(5):487.
[10]Zhang C Q,Chen Y,Yin Y N,et al.A small molecule species specifically inhibits Fusarium myosin I[J].Environmental Microbiology,2015,17(8):2735-2746.
[11]Zheng Z T,Hou Y P,Cai Y Q,et al.Whole-genome sequencing reveals that mutations in myosin-5 confer resistance to the fungicide phenamacril in Fusarium graminearum[J].Scientific Reports,2015,5:8248.
[12]Fan J,Chen F,Diao Y,et al.The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides[J].Plant Pathology,2014,63(4),952-960.
[13]Becher R,Wirsel S G.Fungal cytochrome P450 streol14α-demethylase(CYP51)and azole resistance in plant and human pathogens[J].Applied M icrobiology&Biotechnology,2012,95(4):825-840.
[14]Qian,H W,Duan M L,Sun X M,et al.The binding mechanism betw een azoles and FgCYP51B,sterol14α-demethylase of Fusarium graminearum[J].Pest M anagement Science,2017,74(1):126-134.
[15]Qian,H W,Du J,Chi M Y,et al.The Y137H mutation in the FgCYP51B protein confers reduced sensitivity to tebuconazole in Fusarium graminearum[J].Pest M anagement Science,2017,DOI:10.1002/ps.4837.
[16]Chen C J,Yu J J,Bi C W,et al.Mutations in aβ-tubulin confer resistance of G ibberella zeae to benzimidazole fungicides[J].Phytopathology,2009,99(19):1403-1411.
[17]Zhang H,Li B,Fang Q,et al.SNARE protein FgVam7 controls grow th,asexual and sexual development,and plant infection in Fusarium graminearum[J].Molecular Plant Pathology,2016,17(1):108-119.
[18]Zhu Y Y,Liang X Y,Duan Y B,et al.F240 ofβ2 tubulin explains w hy Fusarium graminearum is less sensitive to carbendazim than Botrytis cinerea[J].Phytopathology,2017,Available at doi:10.1094/PHYTO-09-17-0295-R.
[19]Fan J R.Investigating the function of three paralogous CYP51 gene in Fusarium graminearum[D].Beijing:China Agricultural University,2013.