纳米银石墨烯修饰电极-电化学法测定血清中的过氧化氢
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摘要
建立纳米银–石墨烯修饰电极电化学法测定血清中过氧化氢的方法。在pH 7.0的磷酸缓冲溶液中,过氧化氢在–0.1 V处产生明显的还原峰。过氧化氢在纳米银–石墨烯修饰的电极上的反应是典型的表面控制反应过程。过氧化氢的浓度在0.5～2.7 mmol/L范围内与其还原峰峰电流呈良好的线性关系,线性相关系数r~2=0.993 0,检出限为0.17 mmol/L(信噪比S/N=3),测定结果的相对标准偏差小于5%(n=5),加标回收率为98%～103%。该方法灵敏度高,测定结果准确可靠,可用于血清中过氧化氢的测定。
The method for determination of hydroden peroxide(H_2O_2) in human serum by nanosilver graphene modified electrode–electrochemical method was established. At phosphate buffer solution with pH 7.0,it was found that H_2O_2 had an obvious reductive peak at –0.1 V and the reaction of H_2O_2 on the electrode was the surface control process. The results showed that the concentration of H_2O_2 had good linear relationship with the reductive peak current intensity in the range of 0.5–2.7 mmol/L,the linearly dependent coefficient(r~2) was 0.993 0,the detection limit was 0.17 mmol/L(S/N=3). The relative standard deviation of determination results was less than 5%(n=5) and the average recovery were 98%–103%. The method is acurate with high sensitivity,it can be applied to the determination of H_2O_2 in the human serum.
The method for determination of hydroden peroxide(H_2O_2) in human serum by nanosilver graphene modified electrode–electrochemical method was established. At phosphate buffer solution with pH 7.0,it was found that H_2O_2 had an obvious reductive peak at –0.1 V and the reaction of H_2O_2 on the electrode was the surface control process. The results showed that the concentration of H_2O_2 had good linear relationship with the reductive peak current intensity in the range of 0.5–2.7 mmol/L,the linearly dependent coefficient(r~2) was 0.993 0,the detection limit was 0.17 mmol/L(S/N=3). The relative standard deviation of determination results was less than 5%(n=5) and the average recovery were 98%–103%. The method is acurate with high sensitivity,it can be applied to the determination of H_2O_2 in the human serum.
引文
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[2]MENG W,XU S,DAI L,et al.An enhanced sensitivity towards H2O2 reduction based on a novel Cu metal-organic framework and acetylene black modified electrode[J].Electrochimica acta,2017,230:324-332.
[3]HSU Y K,CHEN Y C,LIN Y G.Spontaneous formation of CuOnanosheets on Cu foil for H2O2 detection[J].Applied surface science,2015,354:85-89.
[4]ASIF M,HAITAO W,SHUANG D,et al.Metal oxide intercalated layered double hydroxide nanosphere:With enhanced electrocatalyic activity towards H2O2 for biological applications[J].Sensors and actuators B:chemical,2017,239:243-252.
[5]HU Y F,ZHANG Z J,YANG C Y.The determination of hydrogen peroxide generated from cigarette smoke with an ultrasensitive and highly selective chemiluminescence method[J].Analytica chimica acta,2007,601(1):95-100.
[6]SUN Y,LUO M,QIN Y,ZHU S,et al.Atomic-thick PtNi nanowires assembled on graphene for high-sensitivity extracellular hydrogen peroxide sensors[J].ACS appl mater interfaces,2017,9(40):34 715-34 721.
[7]TIAN Y,WEI Z,ZHANG K,et al.Three-dimensional phosphorusdoped graphene as an efficient metal-free electrocatalyst for electrochemical sensing[J].Sensors and actuators B:chemical,2017,241:584-591.
[8]LIU M,LIU R,CHEN W.Graphene wrapped Cu2O nanocubes:non-enzymatic electrochemical sensors for the detection of glucose and hydrogen peroxide with enhanced stability[J].Biosens bioelectron,2013,45:206-212.
[9]肖勇,马卫东,孙志勇,等,石墨烯制备与应用研究进展[J].功能材料与器件学报,2017(5):125-131.
[10]黄旭,李双,邢倩荷,等.石墨烯薄膜声源声场特性测试及电路改进[J].电子设计工程,2018,26(8):10-14.
[11]尚永辉,杨会议,周琴,等,异烟肼在多壁碳纳米管修饰碳糊电极上的电化学行为及其应用[J].化学分析计量,2016,25(6):9-12.
[12]CASELLA I G,GIOIA D,RUTILO M.A multi-walled carbon nanotubes/cellulose acetate composite electrode(MWCNT/CA)as sensing probe for the amperometric determination of some catecholamines[J].Sensors and actuators B:chemical,2018,255:3533-3540.
[13]XU Y X,BAI H,LU G W,et al.Flexible graphene films via the filtration of water-soluble noncovalent functionalized graphene sheets[J].Journal of the american chemical society,2008,130(18):5 856-5 857.
[14]QI Y,BAI J,DING X,et al.Electrochemically prepared threedimensional porous nitrogen-doped graphene modified electrode for non-enzymatic detection of hydrogen peroxide[J].Electroanalysis,2017,29(9):2 083-2 089.
[15]祝佳杰.枝状Ag的电化学沉积及对H2O2的电催化性能研究[J].杭州电子科技大学学报,2015,35(6):103-107.