苦瓜中黄酮类物质提取及生物活性分析
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摘要
研究苦瓜中黄酮类物质提取方法,并对提取物质进行生物活性分析.使用冻干法得到苦瓜粉,采用不同的试剂、不同的方法提取其中的黄酮类物质并进行生物活性分析.结果表明:丙酮和75%的乙醇溶液作为提取剂,60℃,浸提和超声波震荡相结合的方法提取效果最好;定性分析结果表明提取物中含有黄酮类物质;生物活性分析结果表明丙酮和75%的乙醇的粗提物及聚酰胺的纯化产物对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌有不同程度的抑制作用,对三种菌的敏感性依次为:大肠杆菌<金黄色葡萄球菌<铜绿假单胞菌.
The extraction methods of flavonoids from bitter Gourd was studied and the biological activity of the extracted material was analyzed in this paper.Bitter Gourd powder was obtained by freezing.Different reagents and methods were studied to extract flavonoids and the biological activity was analyzed. The results show that the best method is using acetone and 75% of ethanol solution as extraction agent,liquid extraction combined with ultrasonic shock extraction at 60 ℃. The results of qualitative analysis show that the extract contains flavonoids.The results of biological activity analysis showed that the crude extracts of acetone and 75 % of ethanol and the purified products of polyamide had different degree of inhibitory effect on Escherichia coli、Staphylococcus aureus、Pseudomonas aeruginosa,and the sensitivity to the three bacteria was:Escherichia coli
The extraction methods of flavonoids from bitter Gourd was studied and the biological activity of the extracted material was analyzed in this paper.Bitter Gourd powder was obtained by freezing.Different reagents and methods were studied to extract flavonoids and the biological activity was analyzed. The results show that the best method is using acetone and 75% of ethanol solution as extraction agent,liquid extraction combined with ultrasonic shock extraction at 60 ℃. The results of qualitative analysis show that the extract contains flavonoids.The results of biological activity analysis showed that the crude extracts of acetone and 75 % of ethanol and the purified products of polyamide had different degree of inhibitory effect on Escherichia coli、Staphylococcus aureus、Pseudomonas aeruginosa,and the sensitivity to the three bacteria was:Escherichia coli
引文
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[5]邢秀芳,于宏芬.纤维素酶在葛根总黄酮提取中的应用[J].中草药,2001,32(1):37.
[6]张英,吴晓琴,俞卓裕.竹叶和银杏叶总黄酮含量及其抗自由基活性的比较研究[J].中国中药杂志,2002,27(4):254-257.
[7]骆祥峰.银杏黄酮类生物活性物质提取工艺研究[D].合肥:合肥工业大学,2000.
[8]洪志伟,孟令宇,葛雅琨,等.槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究[J].吉林化工学院学报,2017,34(5):38-41.
[9]杜文静,谢亚斌,葛雅琨,等.水飞蓟宾对金黄色葡萄球菌和大肠杆菌的体外抑菌作用的研究[J].吉林化工学院学报,2016,33(7):66-69.
[10]林维宣,孙学谦,佟绍芳.紫外分光光度法测定山楂中总黄酮的研究[J].大连轻工业学院学报,1993,12(2):42-50.
[11]冯涛,曹东旭,吕晓玲.竹叶总黄酮含量的测定[J].中国食品添加剂,2002(6):85-87.
[12]张正康.分光光度法测定刺五加片中总黄酮含量[J].时珍国医国药,2001,12(1):41-42.
[2]贾林甫,石建平.药用蔬菜—苦瓜[J].山西农业科学,1988(7):37.
[3]曾涛.苦瓜的营养、药用价值及开发利用[J].中国食物与营养,1998(4):29-30.
[4] Havesteen B.Flavonoids,a Class of Natural Products of High Pharmacological Potency[J]. Biochemical Pharmcology,1983,32(7):1141-1148.
[5]邢秀芳,于宏芬.纤维素酶在葛根总黄酮提取中的应用[J].中草药,2001,32(1):37.
[6]张英,吴晓琴,俞卓裕.竹叶和银杏叶总黄酮含量及其抗自由基活性的比较研究[J].中国中药杂志,2002,27(4):254-257.
[7]骆祥峰.银杏黄酮类生物活性物质提取工艺研究[D].合肥:合肥工业大学,2000.
[8]洪志伟,孟令宇,葛雅琨,等.槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究[J].吉林化工学院学报,2017,34(5):38-41.
[9]杜文静,谢亚斌,葛雅琨,等.水飞蓟宾对金黄色葡萄球菌和大肠杆菌的体外抑菌作用的研究[J].吉林化工学院学报,2016,33(7):66-69.
[10]林维宣,孙学谦,佟绍芳.紫外分光光度法测定山楂中总黄酮的研究[J].大连轻工业学院学报,1993,12(2):42-50.
[11]冯涛,曹东旭,吕晓玲.竹叶总黄酮含量的测定[J].中国食品添加剂,2002(6):85-87.
[12]张正康.分光光度法测定刺五加片中总黄酮含量[J].时珍国医国药,2001,12(1):41-42.