摘要
本研究制备载黑磷量子点(BPQDs)脂质体(liposome-BPQDs),探究其理化性质及用于宫颈癌光热治疗的效果。应用超声法制备BPQDs,薄膜分散法制备liposome-BPQDs,并对其形貌、粒径、电位和拉曼光谱等进行表征。采用CCK-8法检测该纳米粒对人宫颈癌细胞(HeLa)的毒性。使用激光共聚焦显微镜(CLSM)和荧光倒置显微镜分别观察HeLa细胞摄取和细胞凋亡情况。结果表明,扫描电镜下, liposome-BPQDs呈椭球状或球状;透射电镜观察显示liposome-BPQDs粒径约90~110 nm。粒径及电位测量结果表明liposome-BPQDs粒径为(104.2±0.35) nm, zeta电位(-11.3±3.01) mV。脂质体包封率为(84.40±2.13)%。在室外通风、温度范围25℃~34℃和相对湿度80%~82%自然条件下, liposome-BPQDs光热效应良好,降解较BPQDs缓慢。Liposome-BPQDs可被HeLa细胞所摄取;近红外激光照射后,载BPQDs量达20μg·mL~(-1)时, HeLa细胞死亡率大幅度上升。本研究表明, liposomeBPQDs稳定性较高,且具有良好的光热效应,有望应用于宫颈癌光热治疗。
In this study, black phosphorus quantum dots(BPQDs)-loaded liposomes(liposome-BPQDs) were prepared to explore physicochemical properties and photothermal effects on cervical cancer cells. BPQDs were fabricated by ultrasonic method. Liposome-BPQDs were prepared by thin film dispersion. Surface morphology,particle size, zeta potential and Raman spectra of liposome-BPQDs were characterized. The cytotoxicity of the liposome-BPQDs against human cervical cancer cells(HeLa) was examined by CCK-8 assay. Confocal laser scanning microscope(CLSM) and fluorescence microscopy were used to observe the uptake and apoptosis of HeLa cells. The results indicated that liposome-BPQDs were ellipsoidal or spherical under scanning electron microscope,TEM observation showed liposome-BPQDs were about 90-110 nm in diameter. The particle size measurements showed liposome-BPQDs were(104.2 ± 0.35) nm in diameter, and zeta potential were examined to be(-11.3 ±3.01) mV. The encapsulation efficiency was(84.40 ± 2.13)%. Under natural conditions with outdoor ventilation,temperature range of 25 ℃-34 ℃ and relative humidity of 80%-82%, the photothermal effects of liposomeBPQDs was better and the degradation denaturation of liposome-BPQDs were slower than those of BPQDs.The results also reflected that liposome-BPQDs could be uptaken by HeLa cells easily. After near-infrared laser irradiation, the mortality of HeLa cells rise significantly when the amount of BPQDs reach 20 μg·mL~(-1). In summary,liposome-BPQDs with high stability exhibited good photothermal effects, which can be expected to be applied to photothermal therapy of cervical carcinoma.
引文
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