蒙药芯芭中6个环烯醚萜苷的HPLC定量分析
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摘要
目的建立芯芭中6个环烯醚萜苷的含量测定方法。方法采用高效液相色谱法对不同来源芯芭进行含量测定,色谱条件:色谱柱为Inertsil ODS-SP column(4. 6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,检测波长为210 nm,柱温为30℃。结果在选用实验色谱条件下,化合物(S1~S6)的校准曲线表现出良好的线性关系(r> 0. 999 4),平均回收率在95. 0%~98. 0%内,RSD均小于3. 0%。不同来源样品中S1、S2、S3、S4、S5和S6的含量随产地的不同而有显著差异。结论通过实验研究,建立了芯芭中6个环烯醚萜苷的含量测定方法,为综合评价该药材质量提供依据。
OBJECTIVE To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODS Cymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column( 4. 6 mm × 250 mm,5 μm) at 30 ℃ by gradient elution with CH_3 OH-H_2 O as the mobile phase,with the detection wavelength set at 210 nm. RESULTS Under the chromatographic conditions adopted in this study,all the calibration curves exhibited good linearity( r > 0. 999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95. 0%-98. 0%,and the RSD was less than 3%. The contents of all the compounds( S1-S6) in samples from different habitats varied significantly. CONCLUSION The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.
OBJECTIVE To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODS Cymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column( 4. 6 mm × 250 mm,5 μm) at 30 ℃ by gradient elution with CH_3 OH-H_2 O as the mobile phase,with the detection wavelength set at 210 nm. RESULTS Under the chromatographic conditions adopted in this study,all the calibration curves exhibited good linearity( r > 0. 999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95. 0%-98. 0%,and the RSD was less than 3%. The contents of all the compounds( S1-S6) in samples from different habitats varied significantly. CONCLUSION The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.
引文
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[2]ZHAO J P,TA N,HOU M.Determination of luteolin in Cymbaria dahurica by HPLC[J].Chin J Nat Med(中国民族医药杂志),2012,18(5):50-51.
[3]DAI J Q,LIU Z L,YANG L.Non-glycosidic iridoids from Cymbaria mongolica[J].Phytochemistry,2002,59(5):537-542.
[4]LI Z H,LONG P,BAI S,et al.Chemical constituents from Cymbaria dahurica L.(Scrophulariaceae)[J].Biochem Syst Ecol,2014,57(10):11-14.
[5]GUO J H,TIAN C W,LIU X,et al.Advances in research of iridoids occurring in Chinese materia medica[J].Drug Eval Res(药物评价研究),2011,34(4):293-297.
[6]MA Y M,WANG Y.Advances in research of biological activities of iridoids in plants[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,16(17):234-238.
[7]DONG Z,CHEN C X.Research progress on pharmacology of catalpol[J].Chin Tradit Pat Med(中成药),2013,35(5):1047-1051.
[8]CUI J L,GUO T T,WANG M L.Simultaneous determination of five active compounds in wild and culture materials of rhodiola crenulata by RP-HPLC[J].Chin Pharm J(中国药学杂志),2016,51(3):230-233.
[9]WU F M,YANG R S,ZHANG S D,et al.Simultaneous determination of three flavone constituents in Ophiopogonis Radix by HPLC method[J].Chin Pharm J(中国药学杂志),2016,51(8):655-658.
[10]ZOU C C,ZONG Q N,YAN H Y,et al.HPLC fingerprint and quantitative analysis of 6 components of Fructus Trichosanthis and its steamed products[J].Chin Pharm J(中国药学杂志),2017,52(7):597-601.