靖州茯苓三萜类成分对A549细胞中Nrf2信号通路的调控机制研究
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摘要
目的研究靖州茯苓三萜类成分体外对A549细胞增殖的影响及对核因子E2相关因子2(Nrf2)、谷胱甘肽巯基转移酶(GST)、人醌NADH脱氢酶1(NQO1)蛋白表达的影响。方法采用噻唑盐比色(MTT)法检测A549细胞的存活率,Western blot法测定Nrf2,GST,NQO1蛋白表达水平。结果茯苓三萜质量浓度大于15μg/mL时,A549细胞存活率开始降低,且质量浓度与存活率呈负相关;质量浓度大于30μg/mL时,A549细胞中Nrf2,GST,NQO1蛋白表达水平显著升高(P <0. 05),且此时质量浓度与蛋白表达水平呈正相关。结论靖州茯苓三萜类成分可通过下调Nrf2信号通路相关蛋白的表达抑制A549细胞的增殖。
Objective To study the effect of triterpenes from Poria cocos in Jingzhou on the proliferation of A549 cells in vitro and the expression of NF-E2-related factor 2( Nrf2),glutathione S-transferase( GST) and human NAD( P) H dehydrogenase,quinone 1( NQO1)protein. Methods The survival rate of A549 cells was measured by MTT assay, and the expression levels of Nrf2, GST and NQO1 protein were determined by Western blot. Results When the concentration of triterpenes from Poria cocos was more than 15 μg/mL,the survival rate of A549 cells began to decrease, and the concentration of triterpenes was negatively correlated with the survival rate. When the concentration of triterpenes from Poria cocos was more than 30 μg/mL,the expression levels of Nrf2, GST and NQO1 protein in A549 cells increased significantly( P < 0. 05),and the concentration of triterpenes was positively correlated with the expression levels of protein. Conclusion The triterpenes from Poria cocos in Jingzhou can inhibit the proliferation of A549 cells by down-regulating the expression of Nrf2 signaling pathway-related protein.
Objective To study the effect of triterpenes from Poria cocos in Jingzhou on the proliferation of A549 cells in vitro and the expression of NF-E2-related factor 2( Nrf2),glutathione S-transferase( GST) and human NAD( P) H dehydrogenase,quinone 1( NQO1)protein. Methods The survival rate of A549 cells was measured by MTT assay, and the expression levels of Nrf2, GST and NQO1 protein were determined by Western blot. Results When the concentration of triterpenes from Poria cocos was more than 15 μg/mL,the survival rate of A549 cells began to decrease, and the concentration of triterpenes was negatively correlated with the survival rate. When the concentration of triterpenes from Poria cocos was more than 30 μg/mL,the expression levels of Nrf2, GST and NQO1 protein in A549 cells increased significantly( P < 0. 05),and the concentration of triterpenes was positively correlated with the expression levels of protein. Conclusion The triterpenes from Poria cocos in Jingzhou can inhibit the proliferation of A549 cells by down-regulating the expression of Nrf2 signaling pathway-related protein.
引文
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[2]张先淑,胡先明.茯苓三萜化合物的药理作用及临床应用研究进展[J].重庆工贸职业技术学院学报,2011,24(4):46-50.
[3]张敏,高晓红,孙晓萌,等.茯苓的药理作用及研究进展[J].北华大学学报(自然科学版),2008,9(1):63-68.
[4]王晓菲,刘春琰,窦德强.中药茯苓抗肿瘤有效组分研究[J].辽宁中医杂志,2014,41(6):1240-1244.
[5]张新庄,萧伟,徐筱杰,等.基于网络药理学的桂枝茯苓胶囊治疗痛经、子宫肌瘤和盆腔炎的分子作用机制研究[J].中草药,2016,47(1):81-93.
[6]GARG R,GUPTA S,MARU GB.Dietary curcumin modulates transcriptional regulators of phaseⅠand phaseⅡenzymes in benzo[a]pyrene-treated mice:mechanism of its anti-initiating action[J].Carcinogenesis,2008,29(5):1022-1032.
[7]LIU XP,GOLDRING CE,COPPLE IM,et al.Extract of Ginkgo bi-loba induces the phase 2 genes through Keap1-Nrf2-AREsignaling pathway[J].Life Sci,2007,80(17):1586-1591.
[8]KODE A,RAJENDRASOZHAN S,CAITO S,et al.Resveratrol induces glutathione synthesis by activation of Nrf2 and protects against cigarette smoke-mediated oxidative stress in human lung epithelial cells[J].Am J Physiol Lung Cell Mol Physiol,2008,294(3):478-488.
[9]LIAN F,WANG XD.Enzymatic metabolites of lycopene induce Nrf2-mediated expression of phaseⅡdetoxifying/antioxidant enzymes in human bronchial epithelial cells[J].Int J Cancer,2008,123(6):1262-1268.
[10]OKADA K,SHODA J,TAQUCHI K,et al.Ursodeoxycholic acid stimulates Nrf2-mediated hepatocellular transport,detoxification and antioxidative stress systems in mice[J].Am J Physiol Lung Cell Mol Physiol,2008,295(4):735-747.
[11]张方,陈平.超声提取茯苓中三萜类成分的工艺研究[J].武汉工业学院学报,2013,32(4):5-10.
[12]徐硕,姜文清,邝咏梅,等.茯苓的化学成分及生物活性研究进展[J].西北药学杂志,2016,31(3):327-330.
[13]仲兆金,刘浚.茯苓有效成分三萜的研究进展[J].中成药,2001,23(1):58-62.
[14]张强,倪成剑,虞朋余.浙江省乐清市2012-2015年恶性肿瘤发病特征及趋势分析[J].上海预防医学,2017,29(4):300-303.