响应面设计试验法优化沙棘固本速溶茶低温喷雾干燥工艺
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摘要
目的优化沙棘固本速溶茶最佳低温喷雾干燥工艺参数,为工业化生产提供依据。方法采用苯酚-硫酸比色法测定沙棘固本速溶茶干浸膏粉中多糖含量,HPLC测定沙棘固本速溶茶干浸膏粉中人参皂苷Re、Rg1、Rb1的含量,以干浸膏粉质量、多糖含量及人参皂苷Re、Rg1、Rb1含量为评价指标,单因素试验法及响应面设计试验法优化沙棘固本速溶茶低温喷雾干燥工艺参数。结果最优低温喷雾干燥工艺参数如下:药液相对密度1.08 g·mL-1,进风温度120℃,泵药速度20 RPM,风机速度25 m3·h-1,雾化压强0.4 Mpa。结论沙棘固本速溶茶提取浓缩液适合采用低温喷雾干燥法制备干浸膏粉,低温喷雾干燥系瞬间干燥,速度快,对功能性成分影响小;优选的最佳低温喷雾干燥工艺参数科学、合理、可行、成熟稳定,适合工业化生产。
OBJECTIVE To optimize parameters of low-temperature spray-drying process of Sea-buckthorn Strengthening Tea granules, provide the basis for industrialization. METHODS The polysaccharide content in Sea-buckthorn Strengthening Tea granules was measured by phenol-dense sulphuric acid method. The content of ginsenoside Re, Rg1, Rb1 in Sea-buckthorn Strengthening Tea granules were measured by HPLC. The amount percent of weight, polysaccharide content and the content of ginsenoside Re, Rg1, Rb1 of dry granulation were taken as the indexes of low-temperature spray-drying process evaluation. Then the low-temperature spray-drying process parameters of Sea-buckthorn Strengthening Tea granules were optimized by single factor experiment and response surface methodol. RESULTS These low-temperature spray-drying process parameters after optimized were as follows: relative density was 1.08 g·mL-1 in spray-drying, inlet air temperature was 120 ℃, speed of peristaltic pump was 20 RPM, fan speed was 25 m3·h-1, compression pressure was 0.4 Mpa. CONCLUSION Low-temperature spray-drying process is suit for extraction concentrate solution from Sea-buckthorn Strengthening Tea to prepare dry granules.Low-temperature spray-drying have smaller impact on functional ingredient with faster speed. These best low-temperature spray-drying process parameters after optimized of Sea-buckthorn Strengthening Tea are scientific, reasonable, feasible, mature,stable. This optimized low-temperature spray-drying process is suitable for its industrialized production.
OBJECTIVE To optimize parameters of low-temperature spray-drying process of Sea-buckthorn Strengthening Tea granules, provide the basis for industrialization. METHODS The polysaccharide content in Sea-buckthorn Strengthening Tea granules was measured by phenol-dense sulphuric acid method. The content of ginsenoside Re, Rg1, Rb1 in Sea-buckthorn Strengthening Tea granules were measured by HPLC. The amount percent of weight, polysaccharide content and the content of ginsenoside Re, Rg1, Rb1 of dry granulation were taken as the indexes of low-temperature spray-drying process evaluation. Then the low-temperature spray-drying process parameters of Sea-buckthorn Strengthening Tea granules were optimized by single factor experiment and response surface methodol. RESULTS These low-temperature spray-drying process parameters after optimized were as follows: relative density was 1.08 g·mL-1 in spray-drying, inlet air temperature was 120 ℃, speed of peristaltic pump was 20 RPM, fan speed was 25 m3·h-1, compression pressure was 0.4 Mpa. CONCLUSION Low-temperature spray-drying process is suit for extraction concentrate solution from Sea-buckthorn Strengthening Tea to prepare dry granules.Low-temperature spray-drying have smaller impact on functional ingredient with faster speed. These best low-temperature spray-drying process parameters after optimized of Sea-buckthorn Strengthening Tea are scientific, reasonable, feasible, mature,stable. This optimized low-temperature spray-drying process is suitable for its industrialized production.
引文
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[4]XIAO Z W,CHEN G Y,GE Y P,et al.Optimization of extraction process of compound Herb Tea Drink by response surface methodology[J].J Hunan Univ Chin Med(湖南中医药大学学报),2016,36(11):56-59.
[5]ZHONG M,CHEN G Y,YANG P,et al.Optimization of spray drying process for extracted,purified and concentrated liquid of sophora[J].China Pharmacist(中国药师),2015,18(9):1438-1441.
[6]GE Y P,XIAO Z W,LUO K,et al.Study on the desiccation method and forming process of the extracting solution of Instant Herb Tea P[J].J Hunan Univ Chin Med(湖南中医药大学学报),2015,35(5):27-30.
[7]刘艳清,汪洪武,蔡璇,等.响应面法优化栀子总黄酮提取工艺研究[J].中药材,2014,37(2):333-337.
[8]WU Y,XIAO X Y,GE F H.Optimization for supercritical co2extraction with response surface methodology and component analysis of Sapindus mukorossi Oil[J].J Chin Med Mater(中药材),2012,35(2):300-303.
[9]罗堃,周代俊,何述金,等.不同干燥方法对猴头菌-地龙生物转化物水提浓缩液抗凝血活性的影响[J].中药材,2017,40(1):167-170.