武陵山区稻瘟病菌生理小种分子鉴定体系的建立
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摘要
[目的]建立武陵山区稻瘟病菌生理小种分子鉴定体系。[方法]选用RAPD、REMAP、Rep-PCR 3种分子标记和无毒基因检测,对武陵山区稻瘟病菌生理小种文库中2013—2015年分离的220个菌株进行指纹图谱的构建和聚类分析。[结果]共统计26对引物扩增得到的3年样本中稳定出现的97条具有多态性的条带。聚类分析结果显示,在0.75的相似性水平上可将220个菌株分为12个遗传谱系,谱系10为优势谱系,不同年份的稻瘟病菌群体遗传结构存在一定差异,但并未发生明显演化。[结论]建立了新的武陵山区稻瘟病菌生理小种分子鉴定体系,为该地区抗稻瘟病菌品种的选育和布局提供参考。
[Objective]To establish molecular identification system of Magnaporthe oryzae in the Wuling Mountain Area of China.[Method]Marker system including RAPD,REMAP,Rep-PCR and Avr genes was developed for fingerprint mapping and cluster analysis of 220 M.oryzae isolates collected from the Wuling Mountain Area during 2013-2015.[Result]A total of 97 polymorphic bands amplified by 26 pairs of primers were counted.The cluster analysis data showed that 220 strains could be divided into 12 genetic lineages at the 75% similarity level,and the lineage 10 was the dominant lineage.There were some differences in the genetic structure of M.oryzae populations in different years,but no significant evolution occurred.[Conclusion]The study established a new molecular identification system for M.oryzae in the Wuling Mountain Area, and provided a reference for the breeding and distribution of the M.oryzae resistant varieties in this area.
[Objective]To establish molecular identification system of Magnaporthe oryzae in the Wuling Mountain Area of China.[Method]Marker system including RAPD,REMAP,Rep-PCR and Avr genes was developed for fingerprint mapping and cluster analysis of 220 M.oryzae isolates collected from the Wuling Mountain Area during 2013-2015.[Result]A total of 97 polymorphic bands amplified by 26 pairs of primers were counted.The cluster analysis data showed that 220 strains could be divided into 12 genetic lineages at the 75% similarity level,and the lineage 10 was the dominant lineage.There were some differences in the genetic structure of M.oryzae populations in different years,but no significant evolution occurred.[Conclusion]The study established a new molecular identification system for M.oryzae in the Wuling Mountain Area, and provided a reference for the breeding and distribution of the M.oryzae resistant varieties in this area.
引文
[1] 龙飞.水稻常见病虫害防治技术[J].现代农村科技,2018(12):35.
[2] HU K M,QIU D Y,SHEN X L,et al.Isolation and manipulation of quantitative trait loci for disease resistance in rice using a candidate gene approach[J].Molecular plant,2008,1(5):786-793.
[3] KIYOSAWA S.Genetics and epidemiological modeling of breakdown of plant disease resistance[J].Annual review of phytopathology,1982,20(1):93-117.
[4] XU X,YANG W,TIAN K,et al.Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain area of China[J].European journal of plant pathology,2019,153(3):731-742.
[5] 杨小林.湖北省稻瘟病重发区病菌群体致病性分化及水稻抗瘟基因的分析[D].武汉:华中农业大学,2012.
[6] 周延清.DNA分子标记技术在植物研究中的应用[J].生命科学,2005,17(3):15-17.
[7] HUANG J,SI W N,DENG Q M,et al.Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae[J].BMC Genetics,2014,15:1-10.
[8] IMAM J,ALAM S,MANDAL N P,et al.Molecular identification and virulence analysis of AVR genes in rice blast pathogen,Magnaporthe oryzae from Eastern India[J].Euphytica,2015,206(1):21-31.
[9] SELISANA S M,YANORIA M J,QUIME B,et al.Avirulence (AVR) gene-based diagnosis complements existing pathogen surveillance tools for effective deployment of resistance(R) genes against rice blast disease[J].Phytopathology,2017,107(6):711-720.
[10] 徐世秀,秦大宗.武陵山区稻瘟病发病流行特点与防治对策[J].南方农业,2009,3(4):45-46.
[11] 李亮,李晓明.武陵山区水稻的引种与推广[J].南方农业,2015,9(15):56-57.
[12] 李泌,王春台,周杰,等.2011年鄂西地区水稻稻瘟病菌遗传多样性的分析[J].安徽农业科学,2014,42(14):4249-4251.
[13] 田珂,杨武,李泌,等.鄂西南地区2012-2014年稻瘟病菌致病性变化分析[J].华中农业大学学报,2017,36(5):10-14.
[14] 龙子文,周杰,王春台,等.鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定[J].安徽农业科学,2012,40(24):12065-12067.
[15] 杨水英,肖崇刚,杨静,等.重庆稻瘟病菌遗传谱系与生理小种的关系研究[J].西南农业大学学报,2002,24(6):535-538.
[16] 杨小林,施仕胜,张舒,等.湖北省稻瘟病重发区病菌群体致病性分化的研究[J].湖北农业科学,2016,55(16):4169-4171.
[2] HU K M,QIU D Y,SHEN X L,et al.Isolation and manipulation of quantitative trait loci for disease resistance in rice using a candidate gene approach[J].Molecular plant,2008,1(5):786-793.
[3] KIYOSAWA S.Genetics and epidemiological modeling of breakdown of plant disease resistance[J].Annual review of phytopathology,1982,20(1):93-117.
[4] XU X,YANG W,TIAN K,et al.Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain area of China[J].European journal of plant pathology,2019,153(3):731-742.
[5] 杨小林.湖北省稻瘟病重发区病菌群体致病性分化及水稻抗瘟基因的分析[D].武汉:华中农业大学,2012.
[6] 周延清.DNA分子标记技术在植物研究中的应用[J].生命科学,2005,17(3):15-17.
[7] HUANG J,SI W N,DENG Q M,et al.Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae[J].BMC Genetics,2014,15:1-10.
[8] IMAM J,ALAM S,MANDAL N P,et al.Molecular identification and virulence analysis of AVR genes in rice blast pathogen,Magnaporthe oryzae from Eastern India[J].Euphytica,2015,206(1):21-31.
[9] SELISANA S M,YANORIA M J,QUIME B,et al.Avirulence (AVR) gene-based diagnosis complements existing pathogen surveillance tools for effective deployment of resistance(R) genes against rice blast disease[J].Phytopathology,2017,107(6):711-720.
[10] 徐世秀,秦大宗.武陵山区稻瘟病发病流行特点与防治对策[J].南方农业,2009,3(4):45-46.
[11] 李亮,李晓明.武陵山区水稻的引种与推广[J].南方农业,2015,9(15):56-57.
[12] 李泌,王春台,周杰,等.2011年鄂西地区水稻稻瘟病菌遗传多样性的分析[J].安徽农业科学,2014,42(14):4249-4251.
[13] 田珂,杨武,李泌,等.鄂西南地区2012-2014年稻瘟病菌致病性变化分析[J].华中农业大学学报,2017,36(5):10-14.
[14] 龙子文,周杰,王春台,等.鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定[J].安徽农业科学,2012,40(24):12065-12067.
[15] 杨水英,肖崇刚,杨静,等.重庆稻瘟病菌遗传谱系与生理小种的关系研究[J].西南农业大学学报,2002,24(6):535-538.
[16] 杨小林,施仕胜,张舒,等.湖北省稻瘟病重发区病菌群体致病性分化的研究[J].湖北农业科学,2016,55(16):4169-4171.