中山市神湾港鼠形动物携带病原体调查
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摘要
目的掌握中山市神湾港鼠形动物自然感染汉坦病毒、钩端螺旋体、巴尔通体的情况。方法 2017年5月—2018年6月在中山市神湾港货运口岸及神湾游艇客运口岸两个监测点采用鼠笼法捕获鼠形动物,取其肺、肝、脾和肾脏组织。从鼠肺组织中提取总RNA,利用RT-PCR检测汉坦病毒核酸;从肝、脾、肾组织中提取DNA,用PCR方法检测巴尔通体和钩端螺旋体核酸。结果共捕获鼠形动物3种69只,其中臭鼩鼱(Suncus murinus)58只、黄胸鼠(Rattus tanezumi)9只、卡氏小鼠(Mus caroli)2只。汉坦病毒、巴尔通体和钩端螺旋体核酸检测阳性率分别为2.90%、11.59%和4.35%。1只黄胸鼠存在钩端螺旋体、巴尔通体及汉坦病毒的复合感染。结论中山市神湾港鼠形动物存在汉坦病毒、巴尔通体、钩端螺旋体的自然感染,且存在多种病原体复合感染现象,应加强港口区域灭鼠及监测工作。
Objective To understand the natural infection of Hantavirus,Leptospira spp.and Bartonella spp.among rodents at Shenwan port in Zhongshan city.Methods The rodents were captured by cage-traps in Shenwan port area during May 2017 to June 2018.The lung,liver,spleen and kidney samples of rodents were obtained by dissection in laboratory.The total RNA was extracted from lung tissues,and Hantavirus nucleotide acid was amplified by reverse transcription PCR.DNA was extracted from liver,spleen and kidney tissues,were used to detect Bartonella spp.and Leptospira spp.by PCR.ResultsA total of 69 rodents including 58 of Suncus murinus,9 of Rattus tanezumi and 2 of Mus caroli were captured.The positive rate of Hantavirus,Leptospira spp.and Bartonella spp.nucleotide acid was 2.90%,4.35%and 11.59%,respectively.Co-infection with these three pathogens was found in 1Rattus tanezumi.Conclusion The natural infection of Hantavirus,Leptospira spp.and Bartonella spp.were detected in rodents of Shenwan port,and co-infection also existed.It is necessary to strengthen rodents control and surveillance in the port area.
Objective To understand the natural infection of Hantavirus,Leptospira spp.and Bartonella spp.among rodents at Shenwan port in Zhongshan city.Methods The rodents were captured by cage-traps in Shenwan port area during May 2017 to June 2018.The lung,liver,spleen and kidney samples of rodents were obtained by dissection in laboratory.The total RNA was extracted from lung tissues,and Hantavirus nucleotide acid was amplified by reverse transcription PCR.DNA was extracted from liver,spleen and kidney tissues,were used to detect Bartonella spp.and Leptospira spp.by PCR.ResultsA total of 69 rodents including 58 of Suncus murinus,9 of Rattus tanezumi and 2 of Mus caroli were captured.The positive rate of Hantavirus,Leptospira spp.and Bartonella spp.nucleotide acid was 2.90%,4.35%and 11.59%,respectively.Co-infection with these three pathogens was found in 1Rattus tanezumi.Conclusion The natural infection of Hantavirus,Leptospira spp.and Bartonella spp.were detected in rodents of Shenwan port,and co-infection also existed.It is necessary to strengthen rodents control and surveillance in the port area.
引文
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[9]包慧敏,魏跃红,原丽红,等.广州市小型兽类汉坦病毒的基因型[J].热带医学杂志,2018,18(5):565-568.
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[11]蔡春林,陈戊申,陈伟红,等.深圳地区流行性出血热疫点鼠类汉坦病毒血清学调查研究[J].中国卫生检验杂志,2017,27(1):127-128.
[12] Kosoy MY,Regnery RL,Tzianabos T,et al. Distribution,diversity,and host specificity of Bartonella in rodents from the Southeastern United States[J]. American Journal of Tropical Medicine&Hygiene,1997,57(5):578-588.
[13]李雪云,陈经雕,黎聪荣,等.广东省鼠形动物携带巴尔通体的调查和基因特征分析[J].中国人兽共患病学报,2018,34(5):482-486.
[14] Kosoy M,Bai Y,Sheff K,et al. Identification of Bartonella infections in febrile human patients from Thailand and their potential animal reservoirs[J]. American Journal of Tropical Medicine&Hygiene,2010,82(6):1140-1145.
[2]单振菊,刘德星,岳巧云,等.口岸截获国内未见分布种褐小家鼠的DNA条形码鉴定[J].中国媒介生物学及控制杂志,2016,27(1):17-20.
[3]国家质检总局. SN/T 2616-2010国境口岸鼠携带鼠疫杆菌和汉坦病毒快速检测方法[S].北京:中国标准出版社,2010
[4]高玉峰,宋桂荣,谭泽,等.2008—2015年辽宁口岸鼠类及其携带汉坦病毒的监测检测[J].中国国境卫生检疫杂志,2017,40(2):100-103.
[5]国家质检总局. SN/T 4616-2016国境口岸巴尔通体PCR检测方法[S].北京:中国标准出版社,2017.
[6]国家质检总局. SN/T 3741.1-2013国境口岸鼠类携带病原体检测方法:第1部分:致病性钩端螺旋体PCR检测方法[S].北京:中国标准出版社,2014.
[7] Bi Z,Formenty PB,Roth CE. Hantavirus infection:a review and global update[J]. Journal of Infection in Developing Countries,2008,2(1):3.
[8]高云霞,李小波,方盛藩,等.广东口岸鼠形动物间鼠疫耶尔森菌和汉坦病毒感染状况调查[J].中国媒介生物学及控制杂志,2016,27(2):137-140.
[9]包慧敏,魏跃红,原丽红,等.广州市小型兽类汉坦病毒的基因型[J].热带医学杂志,2018,18(5):565-568.
[10]阳帆,何建凡,刘建军,等.应用TaqMan MGB荧光PCR检测鼠肺组织中的汉坦病毒[J].中国卫生检验杂志,2010,20(6):1278-1280.
[11]蔡春林,陈戊申,陈伟红,等.深圳地区流行性出血热疫点鼠类汉坦病毒血清学调查研究[J].中国卫生检验杂志,2017,27(1):127-128.
[12] Kosoy MY,Regnery RL,Tzianabos T,et al. Distribution,diversity,and host specificity of Bartonella in rodents from the Southeastern United States[J]. American Journal of Tropical Medicine&Hygiene,1997,57(5):578-588.
[13]李雪云,陈经雕,黎聪荣,等.广东省鼠形动物携带巴尔通体的调查和基因特征分析[J].中国人兽共患病学报,2018,34(5):482-486.
[14] Kosoy M,Bai Y,Sheff K,et al. Identification of Bartonella infections in febrile human patients from Thailand and their potential animal reservoirs[J]. American Journal of Tropical Medicine&Hygiene,2010,82(6):1140-1145.